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Mol Cell Biol. 1986 November; 6(11): 3606-3613

Gene-sized macronuclear DNA molecules are clustered in micronuclear chromosomes of the ciliate Oxytricha nova.

ABSTRACT

Following the sexual phase of its life cycle, the hypotrichous ciliate Oxytricha nova transforms a copy of its chromosomal micronucleus into a macronucleus containing short, linear DNA molecules with an average size of 2.2 kilobase pairs. In addition, more than 90% of the DNA sequences in the micronuclear genome are eliminated during this process. We have examined the organization of macronuclear DNA molecules in the micronuclear chromosomes. Macronuclear DNA molecules were found to be clustered and separated by less than 550 base pairs in two cloned segments of micronuclear DNA. Recombinant clones of two macronuclear DNA molecules that are adjacent in the micronucleus were also isolated and examined by DNA sequencing. The two macronuclear DNA molecules were found to be separated by only 90 base pairs in the micronuclear genome.

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• Baird, S E, Klobutcher, L A (1989). Characterization of chromosome fragmentation in two protozoans and identification of a candidate fragmentation sequence in Euplotes crassus.. Genes Dev. 3: 585-597 [Abstract]  
• Herrick, G, Hunter, D, Williams, K, Kotter, K (1987). Alternative processing during development of a macronuclear chromosome family in Oxytricha fallax.. Genes Dev. 1: 1047-1058 [Abstract]  
• Ribas-Aparicio, R M, Sparkowski, J J, Proulx, A E, Mitchell, J D, Klobutcher, L A (1987). Nucleic acid splicing events occur frequently during macronuclear development in the protozoan Oxytricha nova and involve the elimination of unique DNA.. Genes Dev. 1: 323-336 [Abstract]