This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gerondakis, S Articles by Bishop, J M Search for Related Content PubMed PubMed Citation Articles by Gerondakis, S Articles by Bishop, J M

 Previous Article  |  Next Article 

Mol Cell Biol. 1986 November; 6(11): 3677-3684

Structure of the protein encoded by the chicken proto-oncogene c-myb.

ABSTRACT

The retroviral oncogene v-myb arose by transduction of the chicken proto-oncogene c-myb. We isolated and sequenced cDNA that represents the entire coding domain of chicken c-myb. By transcribing the cDNA into mRNA in vitro and then translating the RNA, we were able to document the integrity of the cDNA and to identify the codon responsible for initiation of translation from c-myb. Two different alleles of v-myb are extant, one in the genome of avian myeloblastosis virus (AMV) and the other in the genome of erythroblastosis virus 26 (E26V). The proteins encoded by the AMV and E26V alleles of v-myb differ from the product of c-myb in three ways: at their amino termini, they lack 71 and 80 amino acids respectively; at their carboxy termini, they are deficient in 199 and 278 residues; and 11 substitutions of amino acids are scattered throughout the product of AMV allele, whereas the product of the E26V allele contains only a single substitution. The structural origins of tumorigenicity by v-myb and the biological functions of c-myb remain enigmatic. The findings and molecular clones described here should now permit a systematic exploration of these enigmas.

This article has been cited by other articles:

• Lei, W., Liu, F., Ness, S. A. (2005). Positive and negative regulation of c-Myb by cyclin D1, cyclin-dependent kinases, and p27 Kip1. Blood 105: 3855-3861 [Abstract] [Full Text]  
• Karafiat, V., Dvorakova, M., Pajer, P., Kralova, J., Horejsi, Z., Cermak, V., Bartunek, P., Zenke, M., Dvorak, M. (2001). The leucine zipper region of Myb oncoprotein regulates the commitment of hematopoietic progenitors. Blood 98: 3668-3676 [Abstract] [Full Text]  
• Pinson, B., Brendeford, E. M., Gabrielsen, O. S., Daignan-Fornier, B. (2001). Highly conserved features of DNA binding between two divergent members of the Myb family of transcription factors. Nucleic Acids Res 29: 527-535 [Abstract] [Full Text]  
• Katzen, A. L., Jackson, J., Harmon, B. P., Fung, S.-M., Ramsay, G., Bishop, J. M. (1998). Drosophila myb is required for the G2/M transition and maintenance of diploidy. Genes Dev. 12: 831-843 [Abstract] [Full Text]  
• Williams, C. E., Grotewold, E. (1997). Differences between Plant and Animal Myb Domains Are Fundamental for DNA Binding Activity, and Chimeric Myb Domains Have Novel DNA Binding Specificities. J. Biol. Chem. 272: 563-571 [Abstract] [Full Text]  
• Dash, A B, Orrico, F C, Ness, S A (1996). The EVES motif mediates both intermolecular and intramolecular regulation of c-Myb.. Genes Dev. 10: 1858-1869 [Abstract]  
• Pardanaud, L, Luton, D, Prigent, M, Bourcheix, L., Catala, M, Dieterlen-Lievre, F (1996). Two distinct endothelial lineages in ontogeny, one of them related to hemopoiesis. Development 122: 1363-1371 [Abstract]  
• Shapiro, L. H., Shapiro, L. H. (1995). Myb and Ets Proteins Cooperate to Transactivate an Early Myeloid Gene. J. Biol. Chem. 270: 8763-8771 [Abstract] [Full Text]  
• Dubendorff, J W, Whittaker, L J, Eltman, J T, Lipsick, J S (1992). Carboxy-terminal elements of c-Myb negatively regulate transcriptional activation in cis and in trans.. Genes Dev. 6: 2524-2535 [Abstract]  
• Goff, S A, Cone, K C, Fromm, M E (1991). Identification of functional domains in the maize transcriptional activator C1: comparison of wild-type and dominant inhibitor proteins.. Genes Dev. 5: 298-309 [Abstract]  
• Luscher, B, Eisenman, R N (1990). New light on Myc and Myb. Part II. Myb.. Genes Dev. 4: 2235-2241  
• Klempnauer, K H, Arnold, H, Biedenkapp, H (1989). Activation of transcription by v-myb: evidence for two different mechanisms.. Genes Dev. 3: 1582-1589 [Abstract]