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Mol Cell Biol. 1986 November; 6(11): 3973-3983

Developmental regulation of Dictyostelium discoideum actin gene fusions carried on low-copy and high-copy transformation vectors.

D A Knecht, S M Cohen, W F Loomis and H F Lodish

ABSTRACT

The Dictyostelium discoideum genome contains an estimated 17 to 20 actin genes. We report the identification of a new member of this multigene family, actin 15, and its complete nucleotide sequence and transcription initiation sites. We constructed transformation vectors carrying either the actin 15 promoter fused to the neomycin phosphotransferase gene from transposon Tn903 or the actin 6 promoter fused to the neomycin phosphotransferase gene from Tn5. Cells transformed with the actin 15 vector carried less than five copies of vector DNA, while cells transformed with the actin 6 vector carried more than 200 copies. In both cases, the vector appeared to be integrated into the chromosome as a tandem array. Gene fusion RNAs transcribed from the actin 15 and actin 6 vectors were regulated like endogenous actin genes during D. discoideum development. DNA sequences required for temporal and cell type-specific regulation of these genes were contained within 2.8 kilobases of 5' noncoding DNA for actin 15 and 0.7 kilobases of 5' noncoding DNA for actin 6.

Mol Cell Biol. 1986 November; 6(11): 3973-3983




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  • Otsuka, H., Van Haastert, P. J.M. (1998). A novel Myb homolog initiates Dictyostelium development by induction of adenylyl cyclase expression. Genes Dev. 12: 1738-1748 [Abstract] [Full Text]  
  • Shaulsky, G, Fuller, D, Loomis, W. (1998). A cAMP-phosphodiesterase controls PKA-dependent differentiation. Development 125: 691-699 [Abstract]  
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  • Balint-Kurti, P, Ginsburg, G, Rivero-Lezcano, O, Kimmel, A. (1997). rZIP, a RING-leucine zipper protein that regulates cell fate determination during Dictyostelium development. Development 124: 1203-1213 [Abstract]  
  • Abu-Elneel, K., Karchi, M., Ravid, S. (1996). Dictyostelium Myosin II Is Regulated during Chemotaxis by a Novel Protein Kinase C. J. Biol. Chem. 271: 977-984 [Abstract] [Full Text]  
  • Rivero, F, Koppel, B, Peracino, B, Bozzaro, S, Siegert, F, Weijer, C., Schleicher, M, Albrecht, R, Noegel, A. (1996). The role of the cortical cytoskeleton: F-actin crosslinking proteins protect against osmotic stress, ensure cell size, cell shape and motility, and contribute to phagocytosis and development. J. Cell Sci. 109: 2679-2691 [Abstract]  
  • Funamoto, S, Ochiai, H (1996). Antisense RNA inactivation of gene expression of a cell-cell adhesion protein (gp64) in the cellular slime mold Polysphondylium pallidum. J. Cell Sci. 109: 1009-1016 [Abstract]  
  • Parent, C. A., Devreotes, P. N. (1995). Isolation of Inactive and G Protein-resistant Adenylyl Cyclase Mutants Using Random Mutagenesis. J. Biol. Chem. 270: 22693-22696 [Abstract] [Full Text]  
  • Springer, M. L., Patterson, B., Spudich, J. A. (1994). Stage-specific requirement for myosin II during Dictyostelium development. Development 120: 2651-2660 [Abstract]  
  • Schnitzler, G R, Fischer, W H, Firtel, R A (1994). Cloning and characterization of the G-box binding factor, an essential component of the developmental switch between early and late development in Dictyostelium.. Genes Dev. 8: 502-514 [Abstract]  
  • Uyeda, T., Spudich, J. (1993). A functional recombinant myosin II lacking a regulatory light chain-binding site. Science 262: 1867-1870 [Abstract]  
  • Lilly, P, Wu, L, Welker, D L, Devreotes, P N (1993). A G-protein beta-subunit is essential for Dictyostelium development.. Genes Dev. 7: 986-995 [Abstract]  
  • Johnson, R L, Saxe, C L, Gollop, R, Kimmel, A R, Devreotes, P N (1993). Identification and targeted gene disruption of cAR3, a cAMP receptor subtype expressed during multicellular stages of Dictyostelium development.. Genes Dev. 7: 273-282 [Abstract]  
  • Fang, H, Aiba, K, Higa, M, Urushihara, H, Yanagisawa, K (1993). Antisense RNA inactivation of gp138 gene expression results in repression of sexual cell fusion in Dictyostelium discoideum. J. Cell Sci. 106: 785-788 [Abstract]  
  • Richardson, D L, Loomis, W F (1992). Disruption of the sporulation-specific gene spiA in Dictyostelium discoideum leads to spore instability.. Genes Dev. 6: 1058-1070 [Abstract]  
  • Jain, R, Yuen, I S, Taphouse, C R, Gomer, R H (1992). A density-sensing factor controls development in Dictyostelium.. Genes Dev. 6: 390-400 [Abstract]  
  • Anjard, C, Pinaud, S, Kay, R., Reymond, C. (1992). Overexpression of Dd PK2 protein kinase causes rapid development and affects the intracellular cAMP pathway of Dictyostelium discoideum. Development 115: 785-790 [Abstract]  
  • Johnson, R.L., Gundersen, R., Hereld, D., Pitt, G.S., Tugendreich, S., Saxe, C.L., Kimmel, A.R., Devreotes, P.N. (1992). G-Protein-linked Signaling Pathways Mediate Development in Dictyostelium. Cold Spring Harb Symp Quant Biol 57: 169-176 [Abstract]  
  • Firtel, R A, Chapman, A L (1990). A role for cAMP-dependent protein kinase A in early Dictyostelium development.. Genes Dev. 4: 18-28 [Abstract]  
  • Harloff, C, Gerisch, G, Noegel, A A (1989). Selective elimination of the contact site A protein of Dictyostelium discoideum by gene disruption.. Genes Dev. 3: 2011-2019 [Abstract]  
  • Devreotes, P (1989). Dictyostelium discoideum: a model system for cell-cell interactions in development. Science 245: 1054-1058 [Abstract]  
  • Knecht, D., Loomis, W. (1987). Antisense RNA inactivation of myosin heavy chain gene expression in Dictyostelium discoideum. Science 236: 1081-1086 [Abstract]  
  • De Lozanne, A, Spudich, J. (1987). Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination. Science 236: 1086-1091 [Abstract]  


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