This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gilmour, D S
Right arrow Articles by Lis, J T
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gilmour, D S
Right arrow Articles by Lis, J T

 Previous Article  |  Next Article 

Mol Cell Biol. 1986 November; 6(11): 3984-3989

RNA polymerase II interacts with the promoter region of the noninduced hsp70 gene in Drosophila melanogaster cells.

D S Gilmour and J T Lis

ABSTRACT

By using a protein-DNA cross-linking method (D. S. Gilmour and J. T. Lis, Mol. Cell. Biol. 5:2009-2018, 1985), we examined the in vivo distribution of RNA polymerase II on the hsp70 heat shock gene in Drosophila melanogaster Schneider line 2 cells. In heat shock-induced cells, a high level of RNA polymerase II was detected on the entire gene, while in noninduced cells, the RNA polymerase II was confined to the 5' end of the hsp70 gene, predominantly between nucleotides -12 and +65 relative to the start of transcription. This association of RNA polymerase II was apparent whether the cross-linking was performed by a 10-min UV irradiation of chilled cells with mercury vapor lamps or by a 40-microsecond irradiation of cells with a high-energy xenon flash lamp. We hypothesize that RNA polymerase II has access to, and a high affinity for, the promoter region of this gene before induction, and this poised RNA polymerase II may be critical in the mechanism of transcription activation.

Mol Cell Biol. 1986 November; 6(11): 3984-3989




This article has been cited by other articles:

  • Carey, M. F., Peterson, C. L., Smale, S. T. (2009). Chromatin Immunoprecipitation (ChIP). CSH Protocols 2009: pdb.prot5279-pdb.prot5279 [Abstract] [Full Text]  
  • Erturk-Hasdemir, D., Broemer, M., Leulier, F., Lane, W. S., Paquette, N., Hwang, D., Kim, C.-H., Stoven, S., Meier, P., Silverman, N. (2009). Two roles for the Drosophila IKK complex in the activation of Relish and the induction of antimicrobial peptide genes. Proc. Natl. Acad. Sci. USA 106: 9779-9784 [Abstract] [Full Text]  
  • Gilchrist, D. A., Nechaev, S., Lee, C., Ghosh, S. K. B., Collins, J. B., Li, L., Gilmour, D. S., Adelman, K. (2008). NELF-mediated stalling of Pol II can enhance gene expression by blocking promoter-proximal nucleosome assembly. Genes Dev. 22: 1921-1933 [Abstract] [Full Text]  
  • Lee, C., Li, X., Hechmer, A., Eisen, M., Biggin, M. D., Venters, B. J., Jiang, C., Li, J., Pugh, B. F., Gilmour, D. S. (2008). NELF and GAGA Factor Are Linked to Promoter-Proximal Pausing at Many Genes in Drosophila. Mol. Cell. Biol. 28: 3290-3300 [Abstract] [Full Text]  
  • Martinez, C. A., Arnosti, D. N. (2008). Spreading of a Corepressor Linked to Action of Long-Range Repressor Hairy. Mol. Cell. Biol. 28: 2792-2802 [Abstract] [Full Text]  
  • Ni, Z., Saunders, A., Fuda, N. J., Yao, J., Suarez, J.-R., Webb, W. W., Lis, J. T. (2008). P-TEFb Is Critical for the Maturation of RNA Polymerase II into Productive Elongation In Vivo. Mol. Cell. Biol. 28: 1161-1170 [Abstract] [Full Text]  
  • Martowicz, M. L., Grass, J. A., Bresnick, E. H. (2006). GATA-1-mediated Transcriptional Repression Yields Persistent Transcription Factor IIB-Chromatin Complexes. J. Biol. Chem. 281: 37345-37352 [Abstract] [Full Text]  
  • Sulahian, R., Johnston, S. A., Kodadek, T. (2006). The proteasomal ATPase complex is required for stress-induced transcription in yeast. Nucleic Acids Res 34: 1351-1357 [Abstract] [Full Text]  
  • Morris, D. P., Michelotti, G. A., Schwinn, D. A. (2005). Evidence That Phosphorylation of the RNA Polymerase II Carboxyl-terminal Repeats Is Similar in Yeast and Humans. J. Biol. Chem. 280: 31368-31377 [Abstract] [Full Text]  
  • Wade, J. T., Struhl, K. (2004). Association of RNA polymerase with transcribed regions in Escherichia coli. Proc. Natl. Acad. Sci. USA 101: 17777-17782 [Abstract] [Full Text]  
  • Kuhn, E. J., Hart, C. M., Geyer, P. K. (2004). Studies of the Role of the Drosophila scs and scs' Insulators in Defining Boundaries of a Chromosome Puff. Mol. Cell. Biol. 24: 1470-1480 [Abstract] [Full Text]  
  • Schwartz, B. E., Larochelle, S., Suter, B., Lis, J. T. (2003). Cdk7 Is Required for Full Activation of Drosophila Heat Shock Genes and RNA Polymerase II Phosphorylation In Vivo. Mol. Cell. Biol. 23: 6876-6886 [Abstract] [Full Text]  
  • Zhang, L., Johnson, M., Le, K. H., Sato, M., Ilagan, R., Iyer, M., Gambhir, S. S., Wu, L., Carey, M. (2003). Interrogating Androgen Receptor Function in Recurrent Prostate Cancer. Cancer Res. 63: 4552-4560 [Abstract] [Full Text]  
  • Corey, L. L., Weirich, C. S., Benjamin, I. J., Kingston, R. E. (2003). Localized recruitment of a chromatin-remodeling activity by an activator in vivo drives transcriptional elongation. Genes Dev. 17: 1392-1401 [Abstract] [Full Text]  
  • Brown, R. C., Pattison, S., van Ree, J., Coghill, E., Perkins, A., Jane, S. M., Cunningham, J. M. (2002). Distinct Domains of Erythroid Kruppel-Like Factor Modulate Chromatin Remodeling and Transactivation at the Endogenous {beta}-Globin Gene Promoter. Mol. Cell. Biol. 22: 161-170 [Abstract] [Full Text]  
  • Andrulis, E. D., Guzmán, E., Döring, P., Werner, J., Lis, J. T. (2000). High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation. Genes Dev. 14: 2635-2649 [Abstract] [Full Text]  
  • Guzman, E., Lis, J. T. (1999). Transcription Factor TFIIH Is Required for Promoter Melting In Vivo. Mol. Cell. Biol. 19: 5652-5658 [Abstract] [Full Text]  
  • Nasiadka, A, Krause, H. (1999). Kinetic analysis of segmentation gene interactions in Drosophila embryos. Development 126: 1515-1526 [Abstract]  
  • Morimoto, R. I. (1998). Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators. Genes Dev. 12: 3788-3796 [Full Text]  
  • LIS, J. (1998). Promoter-associated Pausing in Promoter Architecture and Postinitiation Transcriptional Regulation. Cold Spring Harb Symp Quant Biol 63: 347-356 [Abstract]  
  • Fujita, M., Kiyono, T., Hayashi, Y., Ishibashi, M. (1997). In Vivo Interaction of Human MCM Heterohexameric Complexes with Chromatin. POSSIBLE INVOLVEMENT OF ATP. J. Biol. Chem. 272: 10928-10935 [Abstract] [Full Text]  
  • Laney, J. D., Biggin, M. D. (1997). Zeste-mediated activation by an enhancer is independent of cooperative DNA binding in vivo. Proc. Natl. Acad. Sci. USA 94: 3602-3604 [Abstract] [Full Text]  
  • Laney, J., Biggin, M. (1996). Redundant control of Ultrabithorax by zeste involves functional levels of zeste protein binding at the Ultrabithorax promoter. Development 122: 2303-2311 [Abstract]  
  • Brown, S A, Imbalzano, A N, Kingston, R E (1996). Activator-dependent regulation of transcriptional pausing on nucleosomal templates.. Genes Dev. 10: 1479-1490 [Abstract]  
  • Shopland, L S, Hirayoshi, K, Fernandes, M, Lis, J T (1995). HSF access to heat shock elements in vivo depends critically on promoter architecture defined by GAGA factor, TFIID, and RNA polymerase II binding sites.. Genes Dev. 9: 2756-2769 [Abstract]  
  • O'Brien, T, Wilkins, R C, Giardina, C, Lis, J T (1995). Distribution of GAGA protein on Drosophila genes in vivo.. Genes Dev. 9: 1098-1110 [Abstract]  
  • Walter, J, Dever, C A, Biggin, M D (1994). Two homeo domain proteins bind with similar specificity to a wide range of DNA sites in Drosophila embryos.. Genes Dev. 8: 1678-1692 [Abstract]  
  • Weeks, J R, Hardin, S E, Shen, J, Lee, J M, Greenleaf, A L (1993). Locus-specific variation in phosphorylation state of RNA polymerase II in vivo: correlations with gene activity and transcript processing.. Genes Dev. 7: 2329-2344 [Abstract]  
  • Hager, G.L., Archer, T.K., Fragoso, G., Bresnick, E.H., Tsukagoshi, Y., John, S., Smith, C.L. (1993). Influence of Chromatin Structure on the Binding of Transcription Factors to DNA. Cold Spring Harb Symp Quant Biol 58: 63-71 [Abstract]  
  • Giardina, C, Perez-Riba, M, Lis, J T (1992). Promoter melting and TFIID complexes on Drosophila genes in vivo.. Genes Dev. 6: 2190-2200 [Abstract]  
  • Krumm, A, Meulia, T, Brunvand, M, Groudine, M (1992). The block to transcriptional elongation within the human c-myc gene is determined in the promoter-proximal region.. Genes Dev. 6: 2201-2213 [Abstract]  
  • Lee, H, Kraus, K W, Wolfner, M F, Lis, J T (1992). DNA sequence requirements for generating paused polymerase at the start of hsp70.. Genes Dev. 6: 284-295 [Abstract]  
  • Champlin, D T, Frasch, M, Saumweber, H, Lis, J T (1991). Characterization of a Drosophila protein associated with boundaries of transcriptionally active chromatin.. Genes Dev. 5: 1611-1621 [Abstract]  
  • Taylor, I C, Workman, J L, Schuetz, T J, Kingston, R E (1991). Facilitated binding of GAL4 and heat shock factor to nucleosomal templates: differential function of DNA-binding domains.. Genes Dev. 5: 1285-1298 [Abstract]  
  • Gilmour, D., Thomas, G., Elgin, S. (1989). Drosophila nuclear proteins bind to regions of alternating C and T residues in gene promoters. Science 245: 1487-1490 [Abstract]  
  • Wu, C, Wilson, S, Walker, B, Dawid, I, Paisley, T, Zimarino, V, Ueda, H (1987). Purification and properties of Drosophila heat shock activator protein. Science 238: 1247-1253 [Abstract]  
  • Yang, X J, Hart, C M, Grayhack, E J, Roberts, J W (1987). Transcription antitermination by phage lambda gene Q protein requires a DNA segment spanning the RNA start site.. Genes Dev. 1: 217-226 [Abstract]  
  • Xie, Y., Sun, L., Kodadek, T. (2000). TATA-binding Protein and the Gal4 Transactivator Do Not Bind to Promoters Cooperatively. J. Biol. Chem. 275: 40797-40803 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»