Previous Article | Next Article 
Mol Cell Biol. 1986 December; 6(12): 4387-4395
High mutation rate of a spleen necrosis virus-based retrovirus vector.
J P Dougherty and
H M Temin
ABSTRACT
Spleen necrosis virus (SNV) is an avian retrovirus that efficiently infects some mammalian cells (e.g., dog and rat cells). We constructed an SNV-based vector, which contains less than 1 kilobase (kb) of the retrovirus sequence, and a number of derivatives containing selectable markers. We obtained high-titer virus stocks, over 10(6) transforming units per ml, with a vector whose genomic RNA consists of 1,850 bases (full-length SNV RNA is 7.7 kb). We also studied two vectors that both carry two genes which should be expressed from a single promoter, one gene from unspliced mRNA and the other gene from spliced mRNA. In one vector, both genes were efficiently expressed as expected. However, in the other vector, expression of the gene 3' to the splice acceptor was inhibited. When we selected for expression of the 3' gene is this latter case, we found that the resistant cells contained mutant proviruses in which the 3' gene could be expressed. Furthermore, we found that mutations were generated during a single round of virus replication (provirus to provirus) at a rate of approximately 0.5% mutations per cycle.
Mol Cell Biol. 1986 December; 6(12): 4387-4395
This article has been cited by other articles:
-
Parveen, Z., Mukhtar, M., Goodrich, A., Acheampong, E., Dornburg, R., Pomerantz, R. J.
(2004). Cross-Packaging of Human Immunodeficiency Virus Type 1 Vector RNA by Spleen Necrosis Virus Proteins: Construction of a New Generation of Spleen Necrosis Virus-Derived Retroviral Vectors. J. Virol.
78: 6480-6488
[Abstract]
[Full Text]
-
Rayet, B., Fan, Y., Gelinas, C.
(2003). Mutations in the v-Rel Transactivation Domain Indicate Altered Phosphorylation and Identify a Subset of NF-{kappa}B-Regulated Cell Death Inhibitors Important for v-Rel Transforming Activity. Mol. Cell. Biol.
23: 1520-1533
[Abstract]
[Full Text]
-
Hu, W.-S., Pathak, V. K.
(2000). Design of Retroviral Vectors and Helper Cells for Gene Therapy. Pharmacol. Rev.
52: 493-512
[Abstract]
[Full Text]
-
Reiser, J., Lai, Z., Zhang, X.-Y., Brady, R. O.
(2000). Development of Multigene and Regulated Lentivirus Vectors. J. Virol.
74: 10589-10599
[Abstract]
[Full Text]
-
Zhang, J., Tang, L.-Y., Li, T., Ma, Y., Sapp, C. M.
(2000). Most Retroviral Recombinations Occur during Minus-Strand DNA Synthesis. J. Virol.
74: 2313-2322
[Abstract]
[Full Text]
-
Chen, C., Agnes, F., Gelinas, C.
(1999). Mapping of a Serine-Rich Domain Essential for the Transcriptional, Antiapoptotic, and Transforming Activities of the v-Rel Oncoprotein. Mol. Cell. Biol.
19: 307-316
[Abstract]
[Full Text]
-
Sachdev, S., Hannink, M.
(1998). Loss of Ikappa Balpha -Mediated Control over Nuclear Import and DNA Binding Enables Oncogenic Activation of c-Rel. Mol. Cell. Biol.
18: 5445-5456
[Abstract]
[Full Text]
-
Sachdev, S., Hoffmann, A., Hannink, M.
(1998). Nuclear Localization of Ikappa Balpha Is Mediated by the Second Ankyrin Repeat: the Ikappa Balpha Ankyrin Repeats Define a Novel Class of cis-Acting Nuclear Import Sequences. Mol. Cell. Biol.
18: 2524-2534
[Abstract]
[Full Text]
-
Ohuchi, H, Takeuchi, J, Yoshioka, H, Ishimaru, Y, Ogura, K, Takahashi, N, Ogura, T, Noji, S
(1998). Correlation of wing-leg identity in ectopic FGF-induced chimeric limbs with the differential expression of chick Tbx5 and Tbx4. Development
125: 51-60
[Abstract]
-
Gourdie, R., Mima, T, Thompson, R., Mikawa, T
(1995). Terminal diversification of the myocyte lineage generates Purkinje fibers of the cardiac conduction system. Development
121: 1423-1431
[Abstract]
-
Zhang, J, Temin, H.
(1993). Rate and mechanism of nonhomologous recombination during a single cycle of retroviral replication. Science
259: 234-238
[Abstract]
-
Hu, W., Temin, H.
(1990). Retroviral recombination and reverse transcription. Science
250: 1227-1233
[Abstract]