This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Orellana, O Articles by Söll, D Search for Related Content PubMed PubMed Citation Articles by Orellana, O Articles by Söll, D

 Previous Article  |  Next Article 

Mol Cell Biol. 1986 February; 6(2): 525-529

The additional guanylate at the 5' terminus of Escherichia coli tRNAHis is the result of unusual processing by RNase P.

ABSTRACT

In eucaryotes the 5'-terminal guanylate moiety of mature tRNAHis is added posttranscriptionally. To determine whether the same mechanism occurs in procaryotes, we processed in vitro-derived Escherichia coli tRNAHis precursors to mature tRNA, either in E. coli extracts or by using pure M1-RNA, the catalytic component of RNase P. The results show that the extra guanylate at the 5' end of mature E. coli tRNAHis is encoded in the gene and is found in tRNA as the result of an unusual cleavage by RNase P.

This article has been cited by other articles:

• Jackman, J. E., Phizicky, E. M. (2006). tRNAHis guanylyltransferase catalyzes a 3'-5' polymerization reaction that is distinct from G-1 addition. Proc. Natl. Acad. Sci. USA 103: 8640-8645 [Abstract] [Full Text]  
• Jackman, J. E., Phizicky, E. M. (2006). tRNAHis guanylyltransferase adds G-1 to the 5' end of tRNAHis by recognition of the anticodon, one of several features unexpectedly shared with tRNA synthetases. RNA 12: 1007-1014 [Abstract] [Full Text]  
• Bullerwell, C. E., Gray, M. W. (2005). In Vitro Characterization of a tRNA Editing Activity in the Mitochondria of Spizellomyces punctatus, a Chytridiomycete Fungus. J. Biol. Chem. 280: 2463-2470 [Abstract] [Full Text]  
• Gu, W., Jackman, J. E., Lohan, A. J., Gray, M. W., Phizicky, E. M. (2003). tRNAHis maturation: An essential yeast protein catalyzes addition of a guanine nucleotide to the 5' end of tRNAHis. Genes Dev. 17: 2889-2901 [Abstract] [Full Text]  
• Persson, T., Cuzic, S., Hartmann, R. K. (2003). Catalysis by RNase P RNA: UNIQUE FEATURES AND UNPRECEDENTED ACTIVE SITE PLASTICITY. J. Biol. Chem. 278: 43394-43401 [Abstract] [Full Text]  
• Forster, A., Altman, S (1990). External guide sequences for an RNA enzyme. Science 249: 783-786 [Abstract]