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Mol Cell Biol. 1986 February; 6(2): 707-709

Detection of UV purine photoproducts in a defined sequence of human DNA.

P E Gallagher and N J Duker

ABSTRACT

The UV-irradiated, 3'-end-labeled, 92-base-pair terminus of the human alphoid sequence was incubated with purified endonuclease v. Previously unreported photoproducts were incised at purine loci. These were not pyrimidine photodimers, 6-4'-(pyrimidin-2'-one)-pyrimidines, base loss sites, or ring-opened purines. Therefore, purine-containing photoproducts, possibly dimers, were incised by the enzyme preparation.

Mol Cell Biol. 1986 February; 6(2): 707-709




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