This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jat, P S Articles by Sharp, P A Search for Related Content PubMed PubMed Citation Articles by Jat, P S Articles by Sharp, P A

 Previous Article  |  Next Article 

Mol Cell Biol. 1986 April; 6(4): 1204-1217

Recombinant retroviruses encoding simian virus 40 large T antigen and polyomavirus large and middle T antigens.

ABSTRACT

We used a murine retrovirus shuttle vector system to construct recombinants capable of constitutively expressing the simian virus 40 (SV40) large T antigen and the polyomavirus large and middle T antigens as well as resistance to G418. Subsequently, these recombinants were used to generate cell lines that produced defective helper-free retroviruses carrying each of the viral oncogenes. These recombinant retroviruses were used to analyze the role of the viral genes in transformation of rat F111 cells. Expression of the polyomavirus middle T antigen alone resulted in cell lines that were highly tumorigenic, whereas expression of the polyomavirus large T resulted in cell lines that were highly tumorigenic, whereas expression of the polyomavirus large T resulted in cell lines that were unaltered by the criteria of morphology, anchorage-independent growth, and tumorigenicity. More surprisingly, SV40 large T-expressing cell lines were not tumorigenic despite the fact that they contained elevated levels of cellular p53 and had a high plating efficiency in soft agar. These results suggest that the SV40 large T antigen is not an acute transforming gene like the polyomavirus middle T antigen but is similar to the establishment genes such as myc and adenovirus EIa.

This article has been cited by other articles:

• Popova, S. N., Barczyk, M., Tiger, C.-F., Beertsen, W., Zigrino, P., Aszodi, A., Miosge, N., Forsberg, E., Gullberg, D. (2007). {alpha}11{beta}1 Integrin-Dependent Regulation of Periodontal Ligament Function in the Erupting Mouse Incisor. Mol. Cell. Biol. 27: 4306-4316 [Abstract] [Full Text]  
• Diaz-Perez, S. V., Ferguson, D. O., Wang, C., Csankovszki, G., Wang, C., Tsai, S.-C., Dutta, D., Perez, V., Kim, S., Eller, C. D., Salstrom, J., Ouyang, Y., Teitell, M. A., Kaltenboeck, B., Chess, A., Huang, S., Marahrens, Y. (2006). A Deletion at the Mouse Xist Gene Exposes Trans-effects That Alter the Heterochromatin of the Inactive X Chromosome and the Replication Time and DNA Stability of Both X Chromosomes. Genetics 174: 1115-1133 [Abstract] [Full Text]  
• Diaz-Perez, S., Ouyang, Y., Perez, V., Cisneros, R., Regelson, M., Marahrens, Y. (2005). The Element(s) at the Nontranscribed Xist Locus of the Active X Chromosome Controls Chromosomal Replication Timing in the Mouse. Genetics 171: 663-672 [Abstract] [Full Text]  
• Yamada, S., Busse, M., Ueno, M., Kelly, O. G., Skarnes, W. C., Sugahara, K., Kusche-Gullberg, M. (2004). Embryonic Fibroblasts with a Gene Trap Mutation in Ext1 Produce Short Heparan Sulfate Chains. J. Biol. Chem. 279: 32134-32141 [Abstract] [Full Text]  
• Akagi, T., Sasai, K., Hanafusa, H. (2003). Refractory nature of normal human diploid fibroblasts with respect to oncogene-mediated transformation. Proc. Natl. Acad. Sci. USA 100: 13567-13572 [Abstract] [Full Text]  
• Chen, H., Detmer, S. A., Ewald, A. J., Griffin, E. E., Fraser, S. E., Chan, D. C. (2003). Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 160: 189-200 [Abstract] [Full Text]  
• Haj, F. G., Markova, B., Klaman, L. D., Bohmer, F. D., Neel, B. G. (2003). Regulation of Receptor Tyrosine Kinase Signaling by Protein Tyrosine Phosphatase-1B. J. Biol. Chem. 278: 739-744 [Abstract] [Full Text]  
• Oostendorp, R. A. J., Medvinsky, A. J., Kusadasi, N., Nakayama, N., Harvey, K., Orelio, C., Ottersbach, K., Covey, T., Ploemacher, R. E., Saris, C., Dzierzak, E. (2002). Embryonal subregion-derived stromal cell lines from novel temperature-sensitive SV40 T antigen transgenic mice support hematopoiesis. J. Cell Sci. 115: 2099-2108 [Abstract] [Full Text]  
• Hahn, W. C., Dessain, S. K., Brooks, M. W., King, J. E., Elenbaas, B., Sabatini, D. M., DeCaprio, J. A., Weinberg, R. A. (2002). Enumeration of the Simian Virus 40 Early Region Elements Necessary for Human Cell Transformation. Mol. Cell. Biol. 22: 2111-2123 [Abstract] [Full Text]  
• Zimonjic, D., Brooks, M. W., Popescu, N., Weinberg, R. A., Hahn, W. C. (2001). Derivation of Human Tumor Cells in Vitro without Widespread Genomic Instability. Cancer Res. 61: 8838-8844 [Abstract] [Full Text]  
• Csankovszki, G., Nagy, A., Jaenisch, R. (2001). Synergism of Xist RNA, DNA Methylation, and Histone Hypoacetylation in Maintaining X Chromosome Inactivation. J. Cell Biol. 153: 773-784 [Abstract] [Full Text]  
• Elenbaas, B., Spirio, L., Koerner, F., Fleming, M. D., Zimonjic, D. B., Donaher, J. L., Popescu, N. C., Hahn, W. C., Weinberg, R. A. (2001). Human breast cancer cells generated by oncogenic transformation of primary mammary epithelial cells. Genes Dev. 15: 50-65 [Abstract] [Full Text]  
• Cherry, S. R., Biniszkiewicz, D., van Parijs, L., Baltimore, D., Jaenisch, R. (2000). Retroviral Expression in Embryonic Stem Cells and Hematopoietic Stem Cells. Mol. Cell. Biol. 20: 7419-7426 [Abstract] [Full Text]  
• Tominaga, K., Morisaki, H., Kaneko, Y., Fujimoto, A., Tanaka, T., Ohtsubo, M., Hirai, M., Okayama, H., Ikeda, K., Nakanishi, M. (1999). Role of Human Cds1 (Chk2) Kinase in DNA Damage Checkpoint and Its Regulation by p53. J. Biol. Chem. 274: 31463-31467 [Abstract] [Full Text]  
• Murrell, J. R., Hunter, D. D. (1999). An Olfactory Sensory Neuron Line, Odora, Properly Targets Olfactory Proteins and Responds to Odorants. J. Neurosci. 19: 8260-8270 [Abstract] [Full Text]  
• Soos, J. M., Ashley, T. A., Morrow, J., Patarroyo, J. C., Szente, B. E., Zamvil, S. S. (1999). Differential expression of B7 co-stimulatory molecules by astrocytes correlates with T cell activation and cytokine production. Int Immunol 11: 1169-1179 [Abstract] [Full Text]  
• Salewski, H., Bayer, T. A., Eidhoff, U., Preuss, U., Weggen, S., Scheidtmann, K. H. (1999). Increased Oncogenicity of Subclones of SV40 Large T-induced Neuroectodermal Tumor Cell Lines after Loss of Large T Expression and Concomitant Mutation in p53. Cancer Res. 59: 1980-1986 [Abstract] [Full Text]  
• Soos, J. M., Morrow, J., Ashley, T. A., Szente, B. E., Bikoff, E. K., Zamvil, S. S. (1998). Astrocytes Express Elements of the Class II Endocytic Pathway and Process Central Nervous System Autoantigen for Presentation to Encephalitogenic T Cells. J. Immunol. 161: 5959-5966 [Abstract] [Full Text]  
• Reuter, A., Nestl, A., Zwacka, R. M., Tuckermann, J., Waldherr, R., Wagner, E.-M., Höyhtyä, M., zum Gottesberge, A. M. M., Angel, P., Weiher, H. (1998). Expression of the Recessive Glomerulosclerosis Gene Mpv17 Regulates MMP-2 Expression in Fibroblasts, the Kidney, and the Inner Ear of Mice. Mol. Biol. Cell 9: 1675-1682 [Abstract] [Full Text]  
• Lloyd, A C, Obermuller, F, Staddon, S, Barth, C F, McMahon, M, Land, H (1997). Cooperating oncogenes converge to regulate cyclin/cdk complexes.. Genes Dev. 11: 663-677 [Abstract]  
• Tucker, K L, Beard, C, Dausmann, J, Jackson-Grusby, L, Laird, P W, Lei, H, Li, E, Jaenisch, R (1996). Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not of nonimprinted genes.. Genes Dev. 10: 1008-1020 [Abstract]  
• Jetten, A., Yankaskas, JR, Stutts, M., Willumsen, N., Boucher, R. (1989). Persistence of abnormal chloride conductance regulation in transformed cystic fibrosis epithelia. Science 244: 1472-1475 [Abstract]