This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hannink, M Articles by Donoghue, D J Search for Related Content PubMed PubMed Citation Articles by Hannink, M Articles by Donoghue, D J

 Previous Article  |  Next Article 

Mol Cell Biol. 1986 April; 6(4): 1304-1314

Deletions in the C-terminal coding region of the v-sis gene: dimerization is required for transformation.

ABSTRACT

The v-sis gene encodes chain B of platelet-derived growth factor. However, this gene codes for additional amino acids at both the N terminus and the C terminus of its gene product which are not present in the amino acid sequence of platelet-derived growth factor. We constructed a series of deletion mutants with deletions in the v-sis gene in order to define the C-terminal limit of the v-sis gene product which is required for transformation. Deletion mutants of the v-sis gene which encoded truncated gene products up to 57 residues shorter than the v-siswt gene product were still able to transform cells. The minimal transforming region of the v-sis gene product contained six residues fewer than were present in chain B of platelet-derived growth factor. Only 10 residues, including the sequence Cys-Lys-Cys, separated the smallest transforming gene product from the largest nontransforming gene product. These cysteine residues were also important for dimerization of the v-sis gene product, since all of the nontransforming v-sis deletions were unable to form dimers when they were analyzed under nonreducing conditions. Our results suggest that there is a strong connection between transformation and dimerization.

This article has been cited by other articles:

• Mercola, M, Deininger, P L, Shamah, S M, Porter, J, Wang, C Y, Stiles, C D (1990). Dominant-negative mutants of a platelet-derived growth factor gene.. Genes Dev. 4: 2333-2341 [Abstract]  
• LaRochelle, W., Giese, N, May-Siroff, M, Robbins, K., Aaronson, S. (1990). Molecular localization of the transforming and secretory properties of PDGF A and PDGF B. Science 248: 1541-1544 [Abstract]  
• Giese, N., Robbins, K., Aaronson, S. (1987). The role of individual cysteine residues in the structure and function of the v-sis gene product. Science 236: 1315-1318 [Abstract]