This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Neckameyer, W S Articles by Wang, L H Search for Related Content PubMed PubMed Citation Articles by Neckameyer, W S Articles by Wang, L H

 Previous Article  |  Next Article 

Mol Cell Biol. 1986 May; 6(5): 1478-1486

Proto-oncogene c-ros codes for a molecule with structural features common to those of growth factor receptors and displays tissue specific and developmentally regulated expression.

ABSTRACT

A recombinant DNA clone containing cellular sequences homologous to the transforming sequence, v-ros, of avian sarcoma virus UR2 was isolated from a chicken genomic DNA library. Heteroduplex mapping and nucleotide sequencing reveal that the v-ros sequences are distributed in nine exons ranging from 65 to 204 nucleotides on cellular ros (c-ros) DNA over a range of 11 kilobases. Comparison of the deduced amino acid sequences of c-ros and v-ros shows two differences: v-ros contains a three-amino-acid insertion within the hydrophobic domain presumed to be involved in membrane association, and (ii) the carboxyl 12 amino acids of v-ros are completely different from those of the deduced c-ros sequence. The deduced amino acid sequence of c-ros bears striking structural features similar to those of insulin and epidermal growth factor receptors, including the presumed hydrophobic membrane binding domain, amino acids flanking the domain, and the distance between the domain and the catalytic region of the kinase activity. The expression of c-ros appears to be under a very stringent control. When tissues at various stages of chicken development were analyzed, only kidney was found to contain a significant level of c-ros RNA. The level of c-ros RNA in kidney tissue is most abundant in 7- to 14-day-old chickens. Finally, nucleotide sequences of c-ros DNA and UR2-associated helper viral genome at regions corresponding to the gag ros recombination site suggest that the junction has been formed by RNA splicing.

This article has been cited by other articles:

• Uttamsingh, S., Zong, C. S., Wang, L.-H. (2003). Matrix-independent Activation of Phosphatidylinositol 3-Kinase, Stat3, and Cyclin A-associated Cdk2 Is Essential for Anchorage-independent Growth of v-Ros-transformed Chicken Embryo Fibroblasts. J. Biol. Chem. 278: 18798-18810 [Abstract] [Full Text]  
• Sonnenberg-Riethmacher, E, Walter, B, Riethmacher, D, Godecke, S, Birchmeier, C (1996). The c-ros tyrosine kinase receptor controls regionalization and differentiation of epithelial cells in the epididymis.. Genes Dev. 10: 1184-1193 [Abstract]  
• O'Bryan, J. P., Fridell, Y.-W., Koski, R., Varnum, B., Liu, E. T. (1995). The Transforming Receptor Tyrosine Kinase, Axl, Is Post-translationally Regulated by Proteolytic Cleavage. J. Biol. Chem. 270: 551-557 [Abstract] [Full Text]  
• Hanks, S., Quinn, A., Hunter, T (1988). The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241: 42-52 [Abstract]  
• Bowtell, D D, Simon, M A, Rubin, G M (1988). Nucleotide sequence and structure of the sevenless gene of Drosophila melanogaster.. Genes Dev. 2: 620-634 [Abstract]  
• Hirai, H, Maru, Y, Hagiwara, K, Nishida, J, Takaku, F (1987). A novel putative tyrosine kinase receptor encoded by the eph gene. Science 238: 1717-1720 [Abstract]  
• Hafen, E, Basler, K, Edstroem, J., Rubin, G. (1987). Sevenless, a cell-specific homeotic gene of Drosophila, encodes a putative transmembrane receptor with a tyrosine kinase domain. Science 236: 55-63 [Abstract]