This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Patterson, M
Right arrow Articles by Rosamond, J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Patterson, M
Right arrow Articles by Rosamond, J

 Previous Article  |  Next Article 

Mol Cell Biol. 1986 May; 6(5): 1590-1598

Molecular characterization of cell cycle gene CDC7 from Saccharomyces cerevisiae.

M Patterson, R A Sclafani, W L Fangman and J Rosamond

ABSTRACT

The product of the CDC7 gene of Saccharomyces cerevisiae appears to have multiple roles in cellular physiology. It is required for the initiation of mitotic DNA synthesis. While it is not required for the initiation of meiotic DNA replication, it is necessary for genetic recombination during meiosis and for the formation of ascospores. It has also been implicated in an error-prone DNA repair pathway. Plasmids capable of complementing temperature-sensitive cdc7 mutations were isolated from libraries of yeast genomic DNA in the multicopy plasmid vectors YRp7 and YEp24. The complementing activity was localized within a 3.0-kilobase genomic DNA fragment. Genetic studies that included integration of the genomic insert at or near the CDC7 locus and marker rescue of four cdc7 alleles proved that the cloned fragment contains the yeast chromosomal CDC7 gene. The RNA transcript of CDC7 is about 1,700 nucleotides. Analysis of the nucleotide sequence of a 2.1-kilobase region of the cloned fragment revealed the presence of an open reading frame of 1,521 nucleotides that is presumed to encode the CDC7 protein. Depending on which of two possible ATG codons initiates translation, the calculated size of the CDC7 protein is 58.2 or 56 kilodaltons. Comparison of the predicted amino acid sequence of the CDC7 gene product with other known protein sequences suggests that CDC7 encodes a protein kinase.

Mol Cell Biol. 1986 May; 6(5): 1590-1598




This article has been cited by other articles:

  • Chou, D. M., Petersen, P., Walter, J. C., Walter, G. (2002). Protein Phosphatase 2A Regulates Binding of Cdc45 to the Prereplication Complex. J. Biol. Chem. 277: 40520-40527 [Abstract] [Full Text]  
  • Lim, R., Winteringham, L. N., Williams, J. H., McCulloch, R. K., Ingley, E., Tiao, J. Y-H., Lalonde, J.-P., Tsai, S., Tilbrook, P. A., Sun, Y., Wu, X., Morris, S. W., Klinken, S. P. (2002). MADM, a Novel Adaptor Protein That Mediates Phosphorylation of the 14-3-3 Binding Site of Myeloid Leukemia Factor 1. J. Biol. Chem. 277: 40997-41008 [Abstract] [Full Text]  
  • Nakamura, T., Nakamura-Kubo, M., Nakamura, T., Shimoda, C. (2002). Novel Fission Yeast Cdc7-Dbf4-Like Kinase Complex Required for the Initiation and Progression of Meiotic Second Division. Mol. Cell. Biol. 22: 309-320 [Abstract] [Full Text]  
  • Sclafani, R. (2000). Cdc7p-Dbf4p becomes famous in the cell cycle. J. Cell Sci. 113: 2111-2117 [Abstract]  
  • Ehrenhofer-Murray, A. E., Kamakaka, R. T., Rine, J. (1999). A Role for the Replication Proteins PCNA, RF-C, Polymerase {epsilon} and Cdc45 in Transcriptional Silencing in Saccharomyces cerevisiae. Genetics 153: 1171-1182 [Abstract] [Full Text]  
  • Oshiro, G., Owens, J. C., Shellman, Y., Sclafani, R. A., Li, J. J. (1999). Cell Cycle Control of Cdc7p Kinase Activity through Regulation of Dbf4p Stability. Mol. Cell. Biol. 19: 4888-4896 [Abstract] [Full Text]  
  • Kumagai, H., Sato, N., Yamada, M., Mahony, D., Seghezzi, W., Lees, E., Arai, K.-I., Masai, H. (1999). A Novel Growth- and Cell Cycle-Regulated Protein, ASK, Activates Human Cdc7-Related Kinase and Is Essential for G1/S Transition in Mammalian Cells. Mol. Cell. Biol. 19: 5083-5095 [Abstract] [Full Text]  
  • Wu, C., Leeuw, T., Leberer, E., Thomas, D. Y., Whiteway, M. (1998). Cell Cycle- and Cln2p-Cdc28p-dependent Phosphorylation of the Yeast Ste20p Protein Kinase. J. Biol. Chem. 273: 28107-28115 [Abstract] [Full Text]  
  • Bousset, K., Diffley, J. F.X. (1998). The Cdc7 protein kinase is required for origin firing during S phase. Genes Dev. 12: 480-490 [Abstract] [Full Text]  
  • Ehrenhofer-Murray, A. E., Gossen, M., Pak, D. T. S., Botchan, M. R., Rine, J. (1995). Separation of Origin Recognition Complex Functions by Cross-Species Complementation. Science 270: 1671-1674 [Abstract]  
  • Hoekstra, M., Liskay, R., Ou, A., DeMaggio, A., Burbee, D., Heffron, F (1991). HRR25, a putative protein kinase from budding yeast: association with repair of damaged DNA. Science 253: 1031-1034 [Abstract]  
  • Din, S, Brill, S J, Fairman, M P, Stillman, B (1990). Cell-cycle-regulated phosphorylation of DNA replication factor A from human and yeast cells.. Genes Dev. 4: 968-977 [Abstract]  
  • Hanks, S., Quinn, A., Hunter, T (1988). The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241: 42-52 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»