This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jaynes, J B
Right arrow Articles by Hauschka, S D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jaynes, J B
Right arrow Articles by Hauschka, S D

 Previous Article  |  Next Article 

Mol Cell Biol. 1986 August; 6(8): 2855-2864

Transcriptional regulation of the muscle creatine kinase gene and regulated expression in transfected mouse myoblasts.

J B Jaynes, J S Chamberlain, J N Buskin, J E Johnson and S D Hauschka

ABSTRACT

The muscle-specific form of creatine kinase (MCK) is induced in differentiating myoblast cultures, and a dramatic increase in mRNA levels precedes and parallels the increase in MCK protein. To study this induction, the complete MCK gene was cloned and characterized. The transcription unit was shown to span 11 kilobases and to contain seven introns. The splice junctions were identified and shown to conform to the appropriate consensus sequences. Close homology with branchpoint consensuses was found upstream of the 3' splice sites in six of seven cases. Transcriptional regulation of the gene in differentiating myoblast cultures was demonstrated by nuclear run-on experiments; increases in transcription accounted for a major part of the increased mRNA levels. Regulated expression of a transfected MCK gene containing the entire transcription unit with 3.3 kilobases of 5'-flanking sequence was also demonstrated during differentiation of the MM14 mouse myoblast cell line. The MCK 5'-flanking region was sufficient to confer transcriptional regulation to a heterologous structural gene, since chloramphenicol acetyl transferase activity was induced during differentiation of cultures transfected with an MCK-chloramphenicol acetyl transferase fusion construct. Examination of the DNA sequence immediately upstream of the transcription start site revealed a 17-nucleotide element which occurred three times. Comparisons with other muscle-specific genes which are also transcriptionally regulated during myogenesis revealed upstream homologies in the alpha-actin and myosin heavy chain genes, but not in the myosin light-chain genes, with the regions containing these repeats. We suggest that coordinate control of a subset of muscle genes may occur via recognition of these common sequences.

Mol Cell Biol. 1986 August; 6(8): 2855-2864




This article has been cited by other articles:

  • Himeda, C. L., Ranish, J. A., Hauschka, S. D. (2008). Quantitative Proteomic Identification of MAZ as a Transcriptional Regulator of Muscle-Specific Genes in Skeletal and Cardiac Myocytes. Mol. Cell. Biol. 28: 6521-6535 [Abstract] [Full Text]  
  • Fornaro, M., Burch, P. M., Yang, W., Zhang, L., Hamilton, C. E., Kim, J. H., Neel, B. G., Bennett, A. M. (2006). SHP-2 activates signaling of the nuclear factor of activated T cells to promote skeletal muscle growth. JCB 175: 87-97 [Abstract] [Full Text]  
  • Burkin, D. J., Wallace, G. Q., Milner, D. J., Chaney, E. J., Mulligan, J. A., Kaufman, S. J. (2005). Transgenic Expression of {alpha}7{beta}1 Integrin Maintains Muscle Integrity, Increases Regenerative Capacity, Promotes Hypertrophy, and Reduces Cardiomyopathy in Dystrophic Mice. Am. J. Pathol. 166: 253-263 [Abstract] [Full Text]  
  • Yuasa, T., Kakuhata, R., Kishi, K., Obata, T., Shinohara, Y., Bando, Y., Izumi, K., Kajiura, F., Matsumoto, M., Ebina, Y. (2004). Platelet-Derived Growth Factor Stimulates Glucose Transport in Skeletal Muscles of Transgenic Mice Specifically Expressing Platelet-Derived Growth Factor Receptor in the Muscle, but It Does Not Affect Blood Glucose Levels. Diabetes 53: 2776-2786 [Abstract] [Full Text]  
  • Lim, W., Neff, E. S., Furlow, J. D. (2004). The mouse muscle creatine kinase promoter faithfully drives reporter gene expression in transgenic Xenopus laevis. Physiol. Genomics 18: 79-86 [Abstract] [Full Text]  
  • Nguyen, Q.-G. V., Buskin, J. N., Himeda, C. L., Shield, M. A., Hauschka, S. D. (2003). Differences in the Function of Three Conserved E-boxes of the Muscle Creatine Kinase Gene in Cultured Myocytes and in Transgenic Mouse Skeletal and Cardiac Muscle. J. Biol. Chem. 278: 46494-46505 [Abstract] [Full Text]  
  • Squire, S., Raymackers, J.M., Vandebrouck, C., Potter, A., Tinsley, J., Fisher, R., Gillis, J.M., Davies, K.E. (2002). Prevention of pathology in mdx mice by expression of utrophin: analysis using an inducible transgenic expression system. Hum Mol Genet 11: 3333-3344 [Abstract] [Full Text]  
  • Couplan, E., Gelly, C., Goubern, M., Fleury, C., Quesson, B., Silberberg, M., Thiaudiere, E., Mateo, P., Lonchampt, M., Levens, N., de Montrion, C., Ortmann, S., Klaus, S., Gonzalez-Barroso, M.-d.-M., Cassard-Doulcier, A.-M., Ricquier, D., Bigard, A. X., Diolez, P., Bouillaud, F. (2002). High Level of Uncoupling Protein 1 Expression in Muscle of Transgenic Mice Selectively Affects Muscles at Rest and Decreases Their IIb Fiber Content. J. Biol. Chem. 277: 43079-43088 [Abstract] [Full Text]  
  • Burkin, D. J., Wallace, G. Q., Nicol, K. J., Kaufman, D. J., Kaufman, S. J. (2001). Enhanced Expression of the {alpha}7{beta}1 Integrin Reduces Muscular Dystrophy and Restores Viability in Dystrophic Mice. JCB 152: 1207-1218 [Abstract] [Full Text]  
  • Briguet, A., Ruegg, M. A. (2000). The Ets Transcription Factor GABP Is Required for Postsynaptic Differentiation In Vivo. J. Neurosci. 20: 5989-5996 [Abstract] [Full Text]  
  • Burkin, D., Kim, J., Gu, M, Kaufman, S. (2000). Laminin and alpha7beta1 integrin regulate agrin-induced clustering of acetylcholine receptors. J. Cell Sci. 113: 2877-2886 [Abstract]  
  • Burkin, D. J., Gu, M., Hodges, B. L., Campanelli, J. T., Kaufman, S. J. (1998). A Functional Role for Specific Spliced Variants of the {alpha}7{beta}1 Integrin in Acetylcholine Receptor Clustering. JCB 143: 1067-1075 [Abstract] [Full Text]  
  • Chen, H.-H., Mack, L. M., Kelly, R., Ontell, M., Kochanek, S., Clemens, P. R. (1997). Persistence in muscle of an adenoviral vector that lacks all viral genes. Proc. Natl. Acad. Sci. USA 94: 1645-1650 [Abstract] [Full Text]  
  • Walke, W., Xiao, G., Goldman, D. (1996). Identification and Characterization of a 47 Base Pair Activity-Dependent Enhancer of the Rat Nicotinic Acetylcholine Receptor delta -Subunit Promoter. J. Neurosci. 16: 3641-3651 [Abstract] [Full Text]  
  • Weintraub, H, Davis, R, Tapscott, S, Thayer, M, Krause, M, Benezra, R, Blackwell, T., Turner, D, Rupp, R, Hollenberg, S, et, al. (1991). The myoD gene family: nodal point during specification of the muscle cell lineage. Science 251: 761-766 [Abstract]  
  • Mueller, P., Wold, B (1989). In vivo footprinting of a muscle specific enhancer by ligation mediated PCR. Science 246: 780-786 [Abstract]  
  • Donoghue, M, Ernst, H, Wentworth, B, Nadal-Ginard, B, Rosenthal, N (1988). A muscle-specific enhancer is located at the 3' end of the myosin light-chain 1/3 gene locus.. Genes Dev. 2: 1779-1790 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»