This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fouser, L A Articles by Friesen, J D Search for Related Content PubMed PubMed Citation Articles by Fouser, L A Articles by Friesen, J D

 Previous Article  |  Next Article 

Mol Cell Biol. 1987 January; 7(1): 225-230

Effects on mRNA splicing of mutations in the 3' region of the Saccharomyces cerevisiae actin intron.

ABSTRACT

Point mutations, deletions, and a sequence context change were introduced at positions 3' to the internal conserved TACTAAC sequence of the Saccharomyces cerevisiae actin intron. In vivo analysis of yeast mRNA splicing suggests that, in contrast to the importance of the polypyrimidine tract in metazoan introns, specific sequences in this region are not required for efficient excision of a yeast intron. However, a double point mutation near the 3' junction (GG/AC) does severely inhibit splicing. Although this mutagenesis of the 3' junction, as well as deletion of most nucleotides between the TACTAAC and the 3' junction, caused only a slight accumulation of primary transcript, the observed accumulation of lariat intermediate by these mutants demonstrates the significance of this region for a step(s) in the splicing process after lariat formation.

This article has been cited by other articles:

• Schwartz, S., Silva, J., Burstein, D., Pupko, T., Eyras, E., Ast, G. (2008). Large-scale comparative analysis of splicing signals and their corresponding splicing factors in eukaryotes. Genome Res 18: 88-103 [Abstract] [Full Text]  
• Weigand, J. E., Suess, B. (2007). Tetracycline aptamer-controlled regulation of pre-mRNA splicing in yeast. Nucleic Acids Res 0: gkm425v1-7 [Abstract] [Full Text]  
• Kapoor, P., Frappier, L. (2003). EBNA1 Partitions Epstein-Barr Virus Plasmids in Yeast Cells by Attaching to Human EBNA1-Binding Protein 2 on Mitotic Chromosomes. J. Virol. 77: 6946-6956 [Abstract] [Full Text]  
• Collins, C. A., Guthrie, C. (1999). Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome. Genes Dev. 13: 1970-1982 [Abstract] [Full Text]  
• Umen, J G, Guthrie, C (1995). A novel role for a U5 snRNP protein in 3' splice site selection.. Genes Dev. 9: 855-868 [Abstract]  
• Deshler, J O, Rossi, J J (1991). Unexpected point mutations activate cryptic 3' splice sites by perturbing a natural secondary structure within a yeast intron.. Genes Dev. 5: 1252-1263 [Abstract]  
• Rymond, B C, Rosbash, M (1988). A chemical modification/interference study of yeast pre-mRNA spliceosome assembly and splicing.. Genes Dev. 2: 428-439 [Abstract]