This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shin, D Y Articles by Ishikawa, T Search for Related Content PubMed PubMed Citation Articles by Shin, D Y Articles by Ishikawa, T

Heat shock response of Saccharomyces cerevisiae mutants altered in cyclic AMP-dependent protein phosphorylation.

ABSTRACT

When Saccharomyces cerevisiae cells grown at 23 degrees C were transferred to 36 degrees C, they initiated synthesis of heat shock proteins, acquired thermotolerance to a lethal heat treatment given after the temperature shift, and arrested their growth transiently at the G1 phase of the cell division cycle. The bcy1 mutant which resulted in production of cyclic AMP (cAMP)-independent protein kinase did not synthesize the three heat shock proteins hsp72A, hsp72B, and hsp41 after the temperature shift. The bcy1 cells failed to acquire thermotolerance to the lethal heat treatment and were not arrested at the G1 phase after the temperature shift. In contrast, the cyr1-2 mutant, which produced a low level of cAMP, constitutively produced three heat shock proteins and four other proteins without the temperature shift and was resistant to the lethal heat treatment. The results suggest that a decrease in the level of cAMP-dependent protein phosphorylation results in the heat shock response, including elevated synthesis of three heat shock proteins, acquisition of thermotolerance, and transient arrest of the cell cycle.

This article has been cited by other articles:

• Bicknell, A. A., Babour, A., Federovitch, C. M., Niwa, M. (2007). A novel role in cytokinesis reveals a housekeeping function for the unfolded protein response. J. Cell Biol. 177: 1017-1027 [Abstract] [Full Text]  
• Gray, J. V., Petsko, G. A., Johnston, G. C., Ringe, D., Singer, R. A., Werner-Washburne, M. (2004). "Sleeping Beauty": Quiescence in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 68: 187-206 [Abstract] [Full Text]  
• Lu, P., Nakorchevskiy, A., Marcotte, E. M. (2003). Expression deconvolution: A reinterpretation of DNA microarray data reveals dynamic changes in cell populations. Proc. Natl. Acad. Sci. USA 100: 10370-10375 [Abstract] [Full Text]  
• Scherz, R., Shinder, V., Engelberg, D. (2001). Anatomical Analysis of Saccharomyces cerevisiae Stalk-Like Structures Reveals Spatial Organization and Cell Specialization. J. Bacteriol. 183: 5402-5413 [Abstract] [Full Text]  
• Johnston, S. D., Enomoto, S., Schneper, L., McClellan, M. C., Twu, F., Montgomery, N. D., Haney, S. A., Broach, J. R., Berman, J. (2001). CAC3 (MSI1) Suppression of RAS2G19V Is Independent of Chromatin Assembly Factor I and Mediated by NPR1. Mol. Cell. Biol. 21: 1784-1794 [Abstract] [Full Text]  
• Lengeler, K. B., Davidson, R. C., D'souza, C., Harashima, T., Shen, W.-C., Wang, P., Pan, X., Waugh, M., Heitman, J. (2000). Signal Transduction Cascades Regulating Fungal Development and Virulence. Microbiol. Mol. Biol. Rev. 64: 746-785 [Abstract] [Full Text]  
• Hong, S.-W., Vierling, E. (2000). Mutants of Arabidopsis thaliana defective in the acquisition of tolerance to high temperature stress. Proc. Natl. Acad. Sci. USA 97: 4392-4397 [Abstract] [Full Text]  
• Stanhill, A., Schick, N., Engelberg, D. (1999). The Yeast Ras/Cyclic AMP Pathway Induces Invasive Growth by Suppressing the Cellular Stress Response. Mol. Cell. Biol. 19: 7529-7538 [Abstract] [Full Text]  
• Li, X., Cai, M. (1999). Recovery of the Yeast Cell Cycle from Heat Shock-induced G1 Arrest Involves a Positive Regulation of G1 Cyclin Expression by the S Phase Cyclin Clb5. J. Biol. Chem. 274: 24220-24231 [Abstract] [Full Text]  
• Yang, Q., Borkovich, K. A. (1999). Mutational Activation of a G{alpha}i Causes Uncontrolled Proliferation of Aerial Hyphae and Increased Sensitivity to Heat and Oxidative Stress in Neurospora crassa. Genetics 151: 107-117 [Abstract] [Full Text]  
• Ma, P., Wera, S., Van Dijck, P., Thevelein, J. M. (1999). The PDE1-encoded Low-Affinity Phosphodiesterase in the Yeast Saccharomyces cerevisiae Has a Specific Function in Controlling Agonist-induced cAMP Signaling. Mol. Biol. Cell 10: 91-104 [Abstract] [Full Text]  
• Görner, W., Durchschlag, E., Martinez-Pastor, M. T., Estruch, F., Ammerer, G., Hamilton, B., Ruis, H., Schüller, C. (1998). Nuclear localization of the C2H2 zinc finger protein Msn2p is regulated by stress and protein kinase A activity. Genes Dev. 12: 586-597 [Abstract] [Full Text]  
• Jenkins, G. M., Richards, A., Wahl, T., Mao, C., Obeid, L., Hannun, Y. (1997). Involvement of Yeast Sphingolipids in the Heat Stress Response of Saccharomyces cerevisiae. J. Biol. Chem. 272: 32566-32572 [Abstract] [Full Text]  
• Kalderon, D, Rubin, G M (1988). Isolation and characterization of Drosophila cAMP-dependent protein kinase genes.. Genes Dev. 2: 1539-1556 [Abstract]  
• Jenkins, G. M., Hannun, Y. A. (2001). Role for de Novo Sphingoid Base Biosynthesis in the Heat-induced Transient Cell Cycle Arrest of Saccharomyces cerevisiae. J. Biol. Chem. 276: 8574-8581 [Abstract] [Full Text]