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Mol Cell Biol. 1987 January; 7(1): 504-511

Sucl+ encodes a predicted 13-kilodalton protein that is essential for cell viability and is directly involved in the division cycle of Schizosaccharomyces pombe.

ABSTRACT

Sucl+ was originally identified as a DNA sequence that, at high copy number, rescued Schizosaccharomyces pombe strains carrying certain temperature-sensitive alleles of the cdc2 cell cycle control gene. We determined the nucleotide sequence of a 1,083-base-pair Sucl+ DNA fragment and S1 mapped its 866-nucleotide RNA transcript. The protein-coding sequence of the gene is interrupted by two intervening sequences of 115 and 51 base pairs. The predicted translational product of the gene is a protein of 13 kilodaltons. A chromosomal gene disruption of Sucl+ was constructed in a diploid S. pombe strain. Germinating spores carrying a null allele of the gene were capable of very limited cell division, following which many cells became highly elongated. The Sucl+ gene was also strongly overexpressed under the control of a heterologous S. pombe promoter. Overexpression of Sucl+ is not lethal but causes a division delay such that cells are approximately twice the normal length at division. These data suggest that Sucl+ encodes a protein which plays a direct role in the cell division cycle of S. pombe.

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• Mendenhall, M. D., Hodge, A. E. (1998). Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1191-1243 [Abstract] [Full Text]  
• Hixon, M. L., Flores, A. I., Wagner, M. W., Gualberto, A. (1998). Ectopic Expression of cdc2/cdc28 Kinase Subunit Homo sapiens 1 Uncouples Cyclin B Metabolism from the Mitotic Spindle Cell Cycle Checkpoint. Mol. Cell. Biol. 18: 6224-6237 [Abstract] [Full Text]  
• Patra, D., Dunphy, W. G. (1998). Xe-p9, a Xenopus Suc1/Cks protein, is essential for the Cdc2-dependent phosphorylation of the anaphase- promoting complex at mitosis. Genes Dev. 12: 2549-2559 [Abstract] [Full Text]  
• Egan, E. A., Solomon, M. J. (1998). Cyclin-Stimulated Binding of Cks Proteins to Cyclin-Dependent Kinases. Mol. Cell. Biol. 18: 3659-3667 [Abstract] [Full Text]  
• Thomas, C. F. Jr., Anders, R. A., Gustafson, M. P., Leof, E. B., Limper, A. H. (1998). Pneumocystis carinii Contains a Functional Cell-division-cycle Cdc2 Homologue. Am. J. Respir. Cell Mol. Bio. 18: 297-306 [Abstract] [Full Text]  
• Neyroz, P., Menna, C., Polverini, E., Masotti, L. (1996). Intrinsic Fluorescence Properties and Structural Analysis of p13suc1 from Schizosaccharomyces pombe. J. Biol. Chem. 271: 27249-27258 [Abstract] [Full Text]  
• Patra, D, Dunphy, W G (1996). Xe-p9, a Xenopus Suc1/Cks homolog, has multiple essential roles in cell cycle control.. Genes Dev. 10: 1503-1515 [Abstract]  
• Moser, M. J., Lee, S. Y., Klevit, R. E., Davis, T. N. (1995). Ca[IMAGE] Binding to Calmodulin and Its Role in Schizosaccharomyces pombe as Revealed by Mutagenesis and NMR Spectroscopy. J. Biol. Chem. 270: 20643-20652 [Abstract] [Full Text]  
• Gavin, A., Cavadore, J., Schorderet-Slatkine, S (1994). Histone H1 kinase activity, germinal vesicle breakdown and M phase entry in mouse oocytes. J. Cell Sci. 107: 275-283 [Abstract]  
• Parge, H., Arvai, A., Murtari, D., Reed, S., Tainer, J. (1993). Human CksHs2 atomic structure: a role for its hexameric assembly in cell cycle control. Science 262: 387-395 [Abstract]  
• Tang, Y, Reed, S I (1993). The Cdk-associated protein Cks1 functions both in G1 and G2 in Saccharomyces cerevisiae.. Genes Dev. 7: 822-832 [Abstract]  
• Giordano, A, Lee, J., Scheppler, J., Herrmann, C, Harlow, E, Deuschle, U, Beach, D, Franza, B. Jr (1991). Cell cycle regulation of histone H1 kinase activity associated with the adenoviral protein E1A. Science 253: 1271-1275 [Abstract]  
• D'Urso, G, Marraccino, R., Marshak, D., Roberts, J. (1990). Cell cycle control of DNA replication by a homologue from human cells of the p34cdc2 protein kinase. Science 250: 786-791 [Abstract]  
• Richardson, H E, Stueland, C S, Thomas, J, Russell, P, Reed, S I (1990). Human cDNAs encoding homologs of the small p34Cdc28/Cdc2-associated protein of Saccharomyces cerevisiae and Schizosaccharomyces pombe.. Genes Dev. 4: 1332-1344 [Abstract]  
• Murray, A., Kirschner, M. (1989). Dominoes and clocks: the union of two views of the cell cycle. Science 246: 614-621 [Abstract]