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Mol Cell Biol. 1987 January; 7(1): 549-551

A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay.

G Akusjärvi, C Svensson and O Nygård

ABSTRACT

The mechanism by which adenovirus virus-associated RNAI stimulates translational efficiency in a transient-expression assay in 293 cells was investigated. We showed that DNA transfection leads to activation of a protein kinase that phosphorylates the alpha subunit of eucaryotic initiation factor 2 and, as a consequence, inhibition of polypeptide chain initiation. Cotransfection of a plasmid encoding adenovirus type 2 virus-associated RNAI recovered the translational capacity by preventing activation of the kinase.

Mol Cell Biol. 1987 January; 7(1): 549-551




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