This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mäkelä, T P Articles by Alitalo, K Search for Related Content PubMed PubMed Citation Articles by Mäkelä, T P Articles by Alitalo, K

Regulation of platelet-derived growth factor gene expression by transforming growth factor beta and phorbol ester in human leukemia cell lines.

Department of Virology, University of Helsinki, Finland.

ABSTRACT

We studied the expression of the genes encoding the A and B chains of platelet-derived growth factor (PDGF) in a number of human leukemia cell lines. Steady-state expression of the A-chain RNA was seen only in the promonocytic leukemia cell line U937 and in the T-cell leukemia cell line MOLT-4. It has previously been reported that both PDGF A and PDGF B genes are induced during megakaryoblastic differentiation of the K562 erythroleukemia cells and transiently during monocytic differentiation of the promyelocytic leukemia cell line HL-60 and U937 cells. In this study we show that PDGF A RNA expression was induced in HL-60 and Jurkat T-cell leukemia cells and increased in U937 and MOLT-4 cells after a 1- to 2-h stimulation with an 8 pM concentration of transforming growth factor beta (TGF-beta). PDGF A RNA remained at a constant, elevated level for at least 24 h in U937 cells, but returned to undetectable levels within 12 h in HL-60 cells. No PDGF A expression was induced by TGF-beta in K562 cells or in lung carcinoma cells (A549). Interestingly, essentially no PDGF B-chain (c-sis proto-oncogene) RNA was expressed simultaneously with PDGF A. In the presence of TGF-beta and protein synthesis inhibitors, PDGF A RNA was superinduced at least 20-fold in the U937 and HL-60 cells. PDGF A expression was accompanied by secretion of immunoprecipitable PDGF to the culture medium of HL-60 and U937 cells. The phorbol ester tumor promoter tetradecanoyl phorbol acetate also increased PDGF A expression with similar kinetics, but with a mechanism distinct from that of TGF-beta. These results suggest a role for TGF-beta in the differential regulation of expression of the PDGF genes.

This article has been cited by other articles:

• Boehrer, S., Ades, L., Braun, T., Galluzzi, L., Grosjean, J., Fabre, C., Le Roux, G., Gardin, C., Martin, A., de Botton, S., Fenaux, P., Kroemer, G. (2008). Erlotinib exhibits antineoplastic off-target effects in AML and MDS: a preclinical study. Blood 111: 2170-2180 [Abstract] [Full Text]  
• Shetty, S., Idell, S. (2000). Post-transcriptional Regulation of Urokinase mRNA. IDENTIFICATION OF A NOVEL UROKINASE mRNA-BINDING PROTEIN IN HUMAN LUNG EPITHELIAL CELLS IN VITRO. J. Biol. Chem. 275: 13771-13779 [Abstract] [Full Text]  
• PIRKKALA, L., ALASTALO, T.-P., NYKÄNEN, P., SEPPÄ, L., SISTONEN, L. (1999). Differentiation lineage-specific expression of human heat shock transcription factor 2. FASEB J. 13: 1089-1098 [Abstract] [Full Text]  
• Maulon, L., Guérin, S., Ricci, J. E., Colin, Y., Cartron, J. P., Auberger, P. (1998). Differential expression of the Kell blood group and CD10 antigens: two related membrane metallopeptidases during differentiation of K562 cells by phorbol ester and hemin. FASEB J. 12: 531-539 [Abstract] [Full Text]