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Mol Cell Biol. 1987 October; 7(10): 3678-3687

Immunofluorescence localization of the Saccharomyces cerevisiae CDC12 gene product to the vicinity of the 10-nm filaments in the mother-bud neck.

B K Haarer and J R Pringle

Department of Biology, University of Michigan, Ann Arbor 48109.

ABSTRACT

Budding cells of the yeast Saccharomyces cerevisiae possess a ring of 10-nm-diameter filaments, of unknown biochemical nature, that lies just inside the plasma membrane in the neck connecting the mother cell to its bud (B. Byers and L. Goetsch, J. Cell Biol. 69:717-721, 1976). Mutants defective in any of four genes (CDC3, CDC10, CDC11, and CDC12) lack these filaments and display a pleiotropic phenotype that involves abnormal bud growth and cell-wall deposition and an inability to complete cytokinesis. We fused the cloned CDC12 gene to the Escherichia coli lacZ and trpE genes and used the resulting fusion proteins to raise polyclonal antibodies specific for the CDC12 gene product. In immunofluorescence experiments with affinity-purified antibodies, the neck region of wild-type and mutant cells stained in patterns consistent with the hypothesis that the CDC12 gene product is a constituent of the ring of 10-nm filaments. Without careful affinity purification of the CDC12-specific antibodies, these staining patterns were completely obscured by the staining of residual cell wall components in the neck by antibodies present even in the "preimmune" sera of all rabbits tested.

Mol Cell Biol. 1987 October; 7(10): 3678-3687




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  • Ayscough, K. R., Stryker, J., Pokala, N., Sanders, M., Crews, P., Drubin, D. G. (1997). High Rates of Actin Filament Turnover in Budding Yeast and Roles for Actin in Establishment and Maintenance of Cell Polarity Revealed Using the Actin Inhibitor Latrunculin-A. JCB 137: 399-416 [Abstract] [Full Text]  
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  • Roemer, T, Madden, K, Chang, J, Snyder, M (1996). Selection of axial growth sites in yeast requires Axl2p, a novel plasma membrane glycoprotein.. Genes Dev. 10: 777-793 [Abstract]  
  • Cvrckova, F, De Virgilio, C, Manser, E, Pringle, J R, Nasmyth, K (1995). Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast.. Genes Dev. 9: 1817-1830 [Abstract]  
  • Pringle, J.R., Bi, E., Harkins, H.A., Zahner, J.E., De Virgilio, C., Chant, J., Corrado, K., Fares, H. (1995). Establishment of Cell Polarity in Yeast. Cold Spring Harb Symp Quant Biol 60: 729-744 [Abstract]  
  • Haarer, B., Petzold, A, Lillie, S., Brown, S. (1994). Identification of MYO4, a second class V myosin gene in yeast. J. Cell Sci. 107: 1055-1064 [Abstract]  
  • Novick, P. J., Goud, B., Salminen, A., Walworth, N.C., Nair, J., Potenza, M. (1988). Regulation of Vesicular Traffic by a GTP-binding Protein on the Cytoplasmic Surface of Secretory Vesicles in Yeast. Cold Spring Harb Symp Quant Biol 53: 637-647 [Abstract]  
  • Kang, P. J., Sanson, A., Lee, B., Park, H.-O. (2001). A GDP/GTP Exchange Factor Involved in Linking a Spatial Landmark to Cell Polarity. Science 292: 1376-1378 [Abstract] [Full Text]  


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