This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rhoads, D D Articles by Roufa, D J Search for Related Content PubMed PubMed Citation Articles by Rhoads, D D Articles by Roufa, D J

 Previous Article  |  Next Article 

Mol Cell Biol. 1987 October; 7(10): 3767-3774

A cloned human ribosomal protein gene functions in rodent cells.

D D Rhoads and D J Roufa

Division of Biology, Kansas State University, Manhattan 66506.

ABSTRACT

Cloned fragments of human ribosomal protein S14 DNA (RPS14) were transfected into cultured Chinese hamster (CHO) cells. Transient expression assays indicated that DNA with as little as 31 base pairs of upstream flanking sequence was transcribed into a polyadenylated, 650-base mRNA that is largely bound to the polyribosomes. In these respects the exogenous human S14 message appeared to function normally in CHO cells. Interestingly, transcription of human RPS14 did not require the TATA sequence located 26 base pairs upstream of exon 1. Stably transformed clones were selected from cultures of emetine-resistant CHO cells (Emr-2) after transfection with pSV2Neo-human RPS14 constructs. Human RPS14 complemented the mutationally based drug resistance of the Chinese hamster cells, demonstrating that the cloned human ribosomal protein gene is functional in rodent cells. Analysis of transformed cells with different amounts of integrated RPS14 indicated that human S14 mRNA levels are not tightly regulated by CHO cells. In contrast, the steady-state S14 level fluctuated only slightly, if at all, in transformed clones whose S14 message contents differed by more than 30-fold. These data support the conclusion that expression of human RPS14 is regulated, at least partially, posttranscriptionally.

---

Mol Cell Biol. 1987 October; 7(10): 3767-3774

This article has been cited by other articles:

• Martin-Nieto, J, Roufa, D. (1997). Functional analysis of human RPS14 null alleles. J. Cell Sci. 110: 955-963 [Abstract]  
• Tasheva, E S, Roufa, D J (1995). Regulation of human RPS14 transcription by intronic antisense RNAs and ribosomal protein S14.. Genes Dev. 9: 304-316 [Abstract]  
• Hammond, M L, Merrick, W, Bowman, L H (1991). Sequences mediating the translation of mouse S16 ribosomal protein mRNA during myoblast differentiation and in vitro and possible control points for the in vitro translation.. Genes Dev. 5: 1723-1736 [Abstract]