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Mol Cell Biol. 1987 November; 7(11): 3916-3922

Differential methylation of the ornithine carbamoyl transferase gene on active and inactive mouse X chromosomes.

Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, Buffalo, New York 14263.

ABSTRACT

Ornithine carbamoyl transferase (Oct) is an X-linked gene which exhibits tissue-specific expression. To determine whether methylation of specific CpG sequences plays a role in dosage compensation or tissue-specific expression of the gene, 13 potentially methylatable sites were identified over a 30-kilobase (kb) region spanning from approximately 15 kb upstream to beyond exon II. Fragments of the Mus hortulanus Oct gene were used as probes to establish the degree of methylation at each site. By considering the methylation status in liver (expressing tissue) versus kidney (nonexpressing tissue) from male and female mice, the active and inactive genes could be investigated on active and inactive X-chromosome backgrounds. One MspI site, 12 kb 5' of the Oct-coding region, was cleaved by HpaII in liver DNA from males but not in kidney DNA from males and thus exhibited complete correlation with tissue-specific expression of the gene. Six other sites showed partial methylation, reflecting incomplete correlation with tissue-specific expression.

This article has been cited by other articles:

• Wu, H, Fassler, R, Schnieke, A, Barker, D, Lee, K., Chapman, V, Francke, U, Jaenisch, R (1992). An X-linked human collagen transgene escapes X inactivation in a subset of cells. Development 116: 687-695 [Abstract]