This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jeannotte, L Articles by Drouin, J Search for Related Content PubMed PubMed Citation Articles by Jeannotte, L Articles by Drouin, J

Tissue-specific activity of the pro-opiomelanocortin gene promoter.

Laboratoire de Biologie Moléculaire des Eucaryotes, Institut de Recherches Cliniques de Montréal, Quebec, Canada.

ABSTRACT

The pro-opiomelanocortin (POMC) gene is specifically expressed in corticotroph cells of the anterior pituitary. To define the POMC promoter sequences responsible for tissue-specific expression, we assessed POMC promoter activity by gene transfer into POMC-expressing pituitary tumor cells (AtT-20) and fibroblast L cells. The rat POMC promoter was only efficiently utilized and correctly transcribed in AtT-20 cells. 5'-End deletion analysis revealed two promoter regions required for activity in AtT-20 cells. When tested by fusion to a heterologous promoter, DNA fragments corresponding to both regions exhibited tissue-specific activity, suggesting the presence of at least two tissue-specific DNA sequence elements within the promoter. In summary, POMC promoter sequences from -480 to -34 base pairs appear sufficient to mimic the specificity of anterior pituitary expression.

This article has been cited by other articles:

• Murakami, I., Takeuchi, S., Kudo, T., Sutou, S., Takahashi, S. (2007). Corticotropin-releasing hormone or dexamethasone regulates rat proopiomelanocortin transcription through Tpit/Pitx-responsive element in its promoter. J Endocrinol 193: 279-290 [Abstract] [Full Text]  
• Latchoumanin, O., Mynard, V., Devin-Leclerc, J., Dugue, M.-A., Bertagna, X., Catelli, M. G. (2007). Reversal of Glucocorticoids-Dependent Proopiomelanocortin Gene Inhibition by Leukemia Inhibitory Factor. Endocrinology 148: 422-432 [Abstract] [Full Text]  
• Nudi, M., Ouimette, J.-F., Drouin, J. (2005). Bone Morphogenic Protein (Smad)-Mediated Repression of Proopiomelanocortin Transcription by Interference with Pitx/Tpit Activity. Mol. Endocrinol. 19: 1329-1342 [Abstract] [Full Text]  
• Mynard, V., Guignat, L., Devin-Leclerc, J., Bertagna, X., Catelli, M. G. (2002). Different Mechanisms for Leukemia Inhibitory Factor-Dependent Activation of Two Proopiomelanocortin Promoter Regions. Endocrinology 143: 3916-3924 [Abstract] [Full Text]  
• Lee, E. J., Martinson, F., Kotlar, T., Thimmapaya, B., Jameson, J. L. (2001). Adenovirus-Mediated Targeted Expression of Toxic Genes to Adrenocorticotropin-Producing Pituitary Tumors Using the Proopiomelanocortin Promoter. J. Clin. Endocrinol. Metab. 86: 3400-3409 [Abstract] [Full Text]  
• Tremblay, J. J., Viger, R. S. (2001). Nuclear Receptor Dax-1 Represses the Transcriptional Cooperation Between GATA-4 and SF-1 in Sertoli Cells. Biol. Reprod. 64: 1191-1199 [Abstract] [Full Text]  
• Tremblay, J. J., Viger, R. S. (1999). Transcription Factor GATA-4 Enhances Mullerian Inhibiting Substance Gene Transcription through a Direct Interaction with the Nuclear Receptor SF-1. Mol. Endocrinol. 13: 1388-1401 [Abstract] [Full Text]  
• Leblond-Francillard, M., Picon, A., Bertagna, X., de Keyzer, Y. (1997). High Expression of the POU Factor Brn3a in Aggressive Neuroendocrine Tumors. J. Clin. Endocrinol. Metab. 82: 89-94 [Abstract] [Full Text]  
• Murphy, E. P., Conneely, O. M. (1997). Neuroendocrine Regulation of the Hypothalamic Pituitary Adrenal Axis by the nurr1/nur77 Subfamily of Nuclear Receptors. Mol. Endocrinol. 11: 39-47 [Abstract] [Full Text]  
• Lamonerie, T, Tremblay, J J, Lanctot, C, Therrien, M, Gauthier, Y, Drouin, J (1996). Ptx1, a bicoid-related homeo box transcription factor involved in transcription of the pro-opiomelanocortin gene.. Genes Dev. 10: 1284-1295 [Abstract]  
• Jansen, E., Ayoubi, T. A. Y., Meulemans, S. M. P., de Ven, W. J. M. V. (1995). Neuroendocrine-specific Expression of the Human Prohormone Convertase 1 Gene. J. Biol. Chem. 270: 15391-15397 [Abstract] [Full Text]