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Mol Cell Biol. 1987 December; 7(12): 4498-4504

Interferon-responsive regulatory elements in the promoter of the human 2',5'-oligo(A) synthetase gene.

P Benech, M Vigneron, D Peretz, M Revel and J Chebath

Department of Virology, Weizmann Institute of Science, Rehovot, Israel.

ABSTRACT

The interferon (IFN)-activated human 2',5'-oligo(A) synthetase E gene contains 11 RNA starts and lacks TATA and CAAT signals. DNA sequences around the promoter make the expression of the chloramphenicol acetyltransferase gene (CAT) inducible over 20-fold by IFN. A 72-base-pair segment (E-IRS) immediately upstream of the RNA starts was defined as being required for IFN-activated expression of the E-gene promoter-CAT constructs and acts in a position-independent manner. It also confers IFN-activated enhancement to the herpes simplex virus thymidine kinase promoter. On this promoter, the 5' part of the E-IRS functions as a constitutive enhancer, while the last 16 base pairs of the E-IRS is sufficient to give IFN-induced expression. On the E-gene promoter, the constitutive enhancer and the IFN-activated sequence are both needed but can be separated. In addition, promoter competition experiments indicate a third regulatory region which helps to repress expression of the E gene in uninduced cells.

Mol Cell Biol. 1987 December; 7(12): 4498-4504




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