This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jones, T R
Right arrow Articles by Cole, M D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jones, T R
Right arrow Articles by Cole, M D

 Previous Article  |  Next Article 

Mol Cell Biol. 1987 December; 7(12): 4513-4521

Rapid cytoplasmic turnover of c-myc mRNA: requirement of the 3' untranslated sequences.

T R Jones and M D Cole

Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, New Jersey 08544.

ABSTRACT

Expression of the c-myc gene can be controlled by transcriptional or posttranscriptional mechanisms (or both), depending on the cell type and the growth conditions. An important mechanism of posttranscriptional regulation is modulation of cytoplasmic c-myc mRNA stability; normal human and murine c-myc mRNAs have cytoplasmic half-lives of 30 min or less. To elucidate the c-myc sequences which impart this unusually high rate of cytoplasmic transcript turnover, we have constructed various deletion and hybrid c-myc genes and analyzed the cytoplasmic stability of the mRNAs produced from them in stably transfected murine fibroblasts. The results indicate that sequences contained within the 5' and 3' ends of the c-myc transcript can affect cytoplasmic stability. Specifically, the 3' untranslated sequences of c-myc exon 3 are required for, but do not ensure, a high rate of transcript turnover in the cytoplasm. Exon 2 coding sequences do not seem to be involved, and exon 1 sequences at the 5' end of the transcript have only a small effect on cytoplasmic transcript stability. The sequences that are primarily responsible for the short c-myc RNA half-life were localized to a region of 140 bases in the 3' untranslated region.

Mol Cell Biol. 1987 December; 7(12): 4513-4521




This article has been cited by other articles:

  • Rohan, J. N. P., Weigel, N. L. (2009). 1{alpha},25-Dihydroxyvitamin D3 Reduces c-Myc Expression, Inhibiting Proliferation and Causing G1 Accumulation in C4-2 Prostate Cancer Cells. Endocrinology 150: 2046-2054 [Abstract] [Full Text]  
  • Sachdeva, M., Zhu, S., Wu, F., Wu, H., Walia, V., Kumar, S., Elble, R., Watabe, K., Mo, Y.-Y. (2009). p53 represses c-Myc through induction of the tumor suppressor miR-145. Proc. Natl. Acad. Sci. USA 106: 3207-3212 [Abstract] [Full Text]  
  • Huerta, M., Munoz, R., Tapia, R., Soto-Reyes, E., Ramirez, L., Recillas-Targa, F., Gonzalez-Mariscal, L., Lopez-Bayghen, E. (2007). Cyclin D1 Is Transcriptionally Down-Regulated by ZO-2 via an E Box and the Transcription Factor c-Myc. Mol. Biol. Cell 18: 4826-4836 [Abstract] [Full Text]  
  • Mallette, F. A., Gaumont-Leclerc, M.-F., Huot, G., Ferbeyre, G. (2007). Myc Down-regulation as a Mechanism to Activate the Rb Pathway in STAT5A-induced Senescence. J. Biol. Chem. 282: 34938-34944 [Abstract] [Full Text]  
  • Escamilla-Powers, J. R., Sears, R. C. (2007). A Conserved Pathway That Controls c-Myc Protein Stability through Opposing Phosphorylation Events Occurs in Yeast. J. Biol. Chem. 282: 5432-5442 [Abstract] [Full Text]  
  • Arnold, H. K., Sears, R. C. (2006). Protein Phosphatase 2A Regulatory Subunit B56{alpha} Associates with c-Myc and Negatively Regulates c-Myc Accumulation.. Mol. Cell. Biol. 26: 2832-2844 [Abstract] [Full Text]  
  • Bachelor, M. A., Bowden, G. T. (2004). Ultraviolet A-induced Modulation of Bcl-XL by p38 MAPK in Human Keratinocytes: POST-TRANSCRIPTIONAL REGULATION THROUGH THE 3'-UNTRANSLATED REGION. J. Biol. Chem. 279: 42658-42668 [Abstract] [Full Text]  
  • Yasuda, S., Wada, S., Arao, Y., Kogawa, M., Kayama, F., Katayama, S. (2004). Interaction between 3' Untranslated Region of Calcitonin Receptor Messenger Ribonucleic Acid (RNA) and Adenylate/Uridylate (AU)-Rich Element Binding Proteins (AU-Rich RNA-Binding Factor 1 and Hu Antigen R). Endocrinology 145: 1730-1738 [Abstract] [Full Text]  
  • Ono, T., Sekino-Suzuki, N., Kikkawa, Y., Yonekawa, H., Kawashima, S. (2003). Alivin 1, a Novel Neuronal Activity-Dependent Gene, Inhibits Apoptosis and Promotes Survival of Cerebellar Granule Neurons. J. Neurosci. 23: 5887-5896 [Abstract] [Full Text]  
  • Wolff, H., Brack-Werner, R., Neumann, M., Werner, T., Schneider, R. (2003). Integrated functional and bioinformatics approach for the identification and experimental verification of RNA signals: application to HIV-1 INS. Nucleic Acids Res 31: 2839-2851 [Abstract] [Full Text]  
  • Pioli, P. A., Hamilton, B. J., Connolly, J. E., Brewer, G., Rigby, W. F. C. (2002). Lactate Dehydrogenase Is an AU-rich Element-binding Protein That Directly Interacts with AUF1. J. Biol. Chem. 277: 35738-35745 [Abstract] [Full Text]  
  • Kanduc, D. (2002). Translational Regulation of Human Papillomavirus Type 16 E7 mRNA by the Peptide SEQIKA, Shared by Rabbit {alpha}1-Globin and Human Cytokeratin 7. J. Virol. 76: 7040-7048 [Abstract] [Full Text]  
  • Sears, R. C., Nevins, J. R. (2002). Signaling Networks That Link Cell Proliferation and Cell Fate. J. Biol. Chem. 277: 11617-11620 [Full Text]  
  • Créancier, L., Mercier, P., Prats, A.-C., Morello, D. (2001). c-myc Internal Ribosome Entry Site Activity Is Developmentally Controlled and Subjected to a Strong Translational Repression in Adult Transgenic Mice. Mol. Cell. Biol. 21: 1833-1840 [Abstract] [Full Text]  
  • Nakagawa, N., Sakurai, N. (2001). Cell Wall Integrity Controls Expression of Endoxyloglucan Transferase in Tobacco BY2 Cells. Plant Cell Physiol 42: 240-244 [Abstract] [Full Text]  
  • Chang, M. M.-J., Juarez, M., Hyde, D. M., Wu, R. (2001). Mechanism of dexamethasone-mediated interleukin-8 gene suppression in cultured airway epithelial cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 280: L107-L115 [Abstract] [Full Text]  
  • Sears, R., Nuckolls, F., Haura, E., Taya, Y., Tamai, K., Nevins, J. R. (2000). Multiple Ras-dependent phosphorylation pathways regulate Myc protein stability. Genes Dev. 14: 2501-2514 [Abstract] [Full Text]  
  • Sastre-Garau, X., Favre, M., Couturier, J., Orth, G. (2000). Distinct patterns of alteration of myc genes associated with integration of human papillomavirus type 16 or type 45 DNA in two genital tumours. J. Gen. Virol. 81: 1983-1993 [Abstract] [Full Text]  
  • Kenealy, M.-R., Flouriot, G., Sonntag-Buck, V., Dandekar, T., Brand, H., Gannon, F. (2000). The 3'-Untranslated Region of the Human Estrogen Receptor {alpha} Gene Mediates Rapid Messenger Ribonucleic Acid Turnover. Endocrinology 141: 2805-2813 [Abstract] [Full Text]  
  • Stoecklin, G., Ming, X.-F., Looser, R., Moroni, C. (2000). Somatic mRNA Turnover Mutants Implicate Tristetraprolin in the Interleukin-3 mRNA Degradation Pathway. Mol. Cell. Biol. 20: 3753-3763 [Abstract] [Full Text]  
  • Nevrivy, D. J., Peterson, V. J., Avram, D., Ishmael, J. E., Hansen, S. G., Dowell, P., Hruby, D. E., Dawson, M. I., Leid, M. (2000). Interaction of GRASP, a Protein encoded by a Novel Retinoic Acid-induced Gene, with Members of the Cytohesin Family of Guanine Nucleotide Exchange Factors. J. Biol. Chem. 275: 16827-16836 [Abstract] [Full Text]  
  • Shetty, S., Idell, S. (2000). Post-transcriptional Regulation of Urokinase mRNA. IDENTIFICATION OF A NOVEL UROKINASE mRNA-BINDING PROTEIN IN HUMAN LUNG EPITHELIAL CELLS IN VITRO. J. Biol. Chem. 275: 13771-13779 [Abstract] [Full Text]  
  • Dixon, D. A., Kaplan, C. D., McIntyre, T. M., Zimmerman, G. A., Prescott, S. M. (2000). Post-transcriptional Control of Cyclooxygenase-2 Gene Expression. THE ROLE OF THE 3'-UNTRANSLATED REGION. J. Biol. Chem. 275: 11750-11757 [Abstract] [Full Text]  
  • Anant, S., Davidson, N. O. (2000). An AU-Rich Sequence Element (UUUN[A/U]U) Downstream of the Edited C in Apolipoprotein B mRNA Is a High-Affinity Binding Site for Apobec-1: Binding of Apobec-1 to This Motif in the 3' Untranslated Region of c-myc Increases mRNA Stability. Mol. Cell. Biol. 20: 1982-1992 [Abstract] [Full Text]  
  • Voeltz, G. K., Steitz, J. A. (1998). AUUUA Sequences Direct mRNA Deadenylation Uncoupled from Decay during Xenopus Early Development. Mol. Cell. Biol. 18: 7537-7545 [Abstract] [Full Text]  
  • Tian, D., Huang, D., Brown, R. C., Jungmann, R. A. (1998). Protein Kinase A Stimulates Binding of Multiple Proteins to a U-Rich Domain in the 3'-Untranslated Region of Lactate Dehydrogenase A mRNA That Is Required for the Regulation of mRNA Stability. J. Biol. Chem. 273: 28454-28460 [Abstract] [Full Text]  
  • Lee, C. H., Leeds, P., Ross, J. (1998). Purification and Characterization of a Polysome-associated Endoribonuclease That Degrades c-myc mRNA in Vitro. J. Biol. Chem. 273: 25261-25271 [Abstract] [Full Text]  
  • Tian, D., Huang, D., Short, S., Short, M. L., Jungmann, R. A. (1998). Protein Kinase A-regulated Instability Site in the 3'-Untranslated Region of Lactate Dehydrogenase-A Subunit mRNA. J. Biol. Chem. 273: 24861-24866 [Abstract] [Full Text]  
  • Yeilding, N. M., Procopio, W. N., Rehman, M. T., Lee, W. M. F. (1998). c-myc mRNA Is Down-regulated during Myogenic Differentiation by Accelerated Decay That Depends on Translation of Regulatory Coding Elements. J. Biol. Chem. 273: 15749-15757 [Abstract] [Full Text]  
  • Russell, J. E., Morales, J., Makeyev, A. V., Liebhaber, S. A. (1998). Sequence Divergence in the 3' Untranslated Regions of Human zeta - and alpha -Globin mRNAs Mediates a Difference in Their Stabilities and Contributes to Efficient alpha -to-zeta Gene Developmental Switching. Mol. Cell. Biol. 18: 2173-2183 [Abstract] [Full Text]  
  • Hamilton, B. J., Burns, C. M., Nichols, R. C., Rigby, W. F. C. (1997). Modulation of AUUUA Response Element Binding by Heterogeneous Nuclear Ribonucleoprotein A1 in Human T Lymphocytes. THE ROLES OF CYTOPLASMIC LOCATION, TRANSCRIPTION, AND PHOSPHORYLATION. J. Biol. Chem. 272: 28732-28741 [Abstract] [Full Text]  
  • Wada, S., Udagawa, N., Akatsu, T., Nagata, N., Martin, T. J., Findlay, D. M. (1997). Regulation by Calcitonin and Glucocorticoids of Calcitonin Receptor Gene Expression in Mouse Osteoclasts. Endocrinology 138: 521-529 [Abstract] [Full Text]  
  • Koishi, R., Kawashima, I., Yoshimura, C., Sugawara, M., Serizawa, N. (1997). Cloning and Characterization of a Novel Oxidoreductase KDRF from a Human Bone Marrow-derived Stromal Cell Line KM-102. J. Biol. Chem. 272: 2570-2577 [Abstract] [Full Text]  
  • Chagnovich, D., Cohn, S. L. (1996). Binding of a 40-kDa Protein to the N-myc 3'-Untranslated Region Correlates with Enhanced N-myc Expression in Human Neuroblastoma. J. Biol. Chem. 271: 33580-33586 [Abstract] [Full Text]  
  • Winzen, R., Kafert, S., Mylius-Spencker, B. P. H. A., Resch, K., Holtmann, H. (1996). Interaction between the mRNA of the 55-kDa Tumor Necrosis Factor Receptor and Cellular Proteins. POSSIBLE INVOLVEMENT IN POST-TRANSCRIPTIONAL REGULATION OF RECEPTOR EXPRESSION. J. Biol. Chem. 271: 13461-13467 [Abstract] [Full Text]  
  • DeMaria, C. T., Brewer, G. (1996). AUF1 Binding Affinity to A+U-rich Elements Correlates with Rapid mRNA Degradation. J. Biol. Chem. 271: 12179-12184 [Abstract] [Full Text]  
  • Ma, W.-J., Cheng, S., Campbell, C., Wright, A., Furneaux, H. (1996). Cloning and Characterization of HuR, a Ubiquitously Expressed Elav-like Protein. J. Biol. Chem. 271: 8144-8151 [Abstract] [Full Text]  
  • Ohtsuka, T., Kubota, A., Hirano, T., Watanabe, K., Yoshida, H., Tsurufuji, M., Iizuka, Y., Konishi, K., Tsurufuji, S. (1996). Glucocorticoid-mediated Gene Suppression of Rat Cytokine-induced Neutrophil Chemoattractant CINC/gro, a Member of the Interleukin-8 Family, through Impairment of NF-kappaB Activation. J. Biol. Chem. 271: 1651-1659 [Abstract] [Full Text]  
  • Marksitzer, R., Stief, A., Menoud, P.-A., Nagamine, Y. (1995). Role of LFB3 in Cell-specific cAMP Induction of the Urokinase-type Plasminogen Activator Gene. J. Biol. Chem. 270: 21833-21838 [Abstract] [Full Text]  
  • Tholanikunnel, B. G., Granneman, J. G., Malbon, C. C. (1995). The M[IMAGE] 35,000 [IMAGE]-Adrenergic Receptor mRNA-binding Protein Binds Transcripts of G-protein-linked Receptors Which Undergo Agonist-induced Destabilization. J. Biol. Chem. 270: 12787-12793 [Abstract] [Full Text]  
  • Nagy, E., Rigby, W. F. C. (1995). Glyceraldehyde-3-phosphate Dehydrogenase Selectively Binds AU-rich RNA in the NAD[IMAGE]-binding Region (Rossmann Fold). J. Biol. Chem. 270: 2755-2763 [Abstract] [Full Text]  
  • Kramatschek, B, Campos-Ortega, J. (1994). Neuroectodermal transcription of the Drosophila neurogenic genes E(spl) and HLH-m5 is regulated by proneural genes. Development 120: 815-826 [Abstract]  
  • Pulak, R, Anderson, P (1993). mRNA surveillance by the Caenorhabditis elegans smg genes.. Genes Dev. 7: 1885-1897 [Abstract]  
  • Takahama, Y, Singer, A (1992). Post-transcriptional regulation of early T cell development by T cell receptor signals. Science 258: 1456-1462 [Abstract]  
  • Bernstein, P L, Herrick, D J, Prokipcak, R D, Ross, J (1992). Control of c-myc mRNA half-life in vitro by a protein capable of binding to a coding region stability determinant.. Genes Dev. 6: 642-654 [Abstract]  
  • Shyu, A B, Belasco, J G, Greenberg, M E (1991). Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay.. Genes Dev. 5: 221-231 [Abstract]  
  • Wisdom, R, Lee, W (1991). The protein-coding region of c-myc mRNA contains a sequence that specifies rapid mRNA turnover and induction by protein synthesis inhibitors.. Genes Dev. 5: 232-243 [Abstract]  
  • Fox, C A, Wickens, M (1990). Poly(A) removal during oocyte maturation: a default reaction selectively prevented by specific sequences in the 3' UTR of certain maternal mRNAs.. Genes Dev. 4: 2287-2298 [Abstract]  
  • Richman, A, Hayday, A (1989). Normal expression of a rearranged and mutated c-myc oncogene after transfection into fibroblasts. Science 246: 494-497 [Abstract]  
  • Detich, N., Ramchandani, S., Szyf, M. (2001). A Conserved 3'-Untranslated Element Mediates Growth Regulation of DNA Methyltransferase 1 and Inhibits Its Transforming Activity. J. Biol. Chem. 276: 24881-24890 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»