This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Neigeborn, L Articles by Carlson, M Search for Related Content PubMed PubMed Citation Articles by Neigeborn, L Articles by Carlson, M

SSN20 is an essential gene with mutant alleles that suppress defects in SUC2 transcription in Saccharomyces cerevisiae.

ABSTRACT

Dominant and recessive mutations at the SSN20 locus were previously isolated as extragenic suppressors of mutations in three genes (SNF2, SNF5, and SNF6) that are required in trans to derepress invertase expression. All ssn20 alleles cause recessive, temperature-sensitive lethality. In this study we cloned the SSN20 gene, identified a 4.6-kilobase poly(A)-containing RNA, and showed that disruption of the gene is lethal in a haploid cell. Genetic mapping of SSN20 to a locus on chromosome VII 10 centimorgans distal to cly8 led to the finding that SSN20 is the same gene as SPT6, which affects expression of delta insertions in the 5' noncoding region of HIS4 (F. Winston, D. T. Chaleff, B. Valent, and G. R. Fink, Genetics 107:179-197, 1984). We also showed that an ssn20 mutation restored expression of secreted invertase from deletions of the SUC2 upstream regulatory region; ssn20 restored derepression of SUC2 mRNA in strains with a SUC2 upstream region deletion or a snf2 mutation. Increased or decreased gene dosage of SSN20 also suppressed defects that are suppressed by ssn20 missense mutations. These findings suggest that SSN20 plays a role in general transcriptional processes.

This article has been cited by other articles:

• Prather, D., Krogan, N. J., Emili, A., Greenblatt, J. F., Winston, F. (2005). Identification and Characterization of Elf1, a Conserved Transcription Elongation Factor in Saccharomyces cerevisiae. Mol. Cell. Biol. 25: 10122-10135 [Abstract] [Full Text]  
• Keogh, M.-C., Podolny, V., Buratowski, S. (2003). Bur1 Kinase Is Required for Efficient Transcription Elongation by RNA Polymerase II. Mol. Cell. Biol. 23: 7005-7018 [Abstract] [Full Text]  
• Kaplan, C. D., Laprade, L., Winston, F. (2003). Transcription Elongation Factors Repress Transcription Initiation from Cryptic Sites. Science 301: 1096-1099 [Abstract] [Full Text]  
• Geng, F., Cao, Y., Laurent, B. C. (2001). Essential Roles of Snf5p in Snf-Swi Chromatin Remodeling In Vivo. Mol. Cell. Biol. 21: 4311-4320 [Abstract] [Full Text]  
• Evans, D. R. H., Brewster, N. K., Xu, Q., Rowley, A., Altheim, B. A., Johnston, G. C., Singer, R. A. (1998). The Yeast Protein Complex Containing Cdc68 and Pob3 Mediates Core-Promoter Repression Through the Cdc68 N-Terminal Domain. Genetics 150: 1393-1405 [Abstract] [Full Text]  
• Du, J., Nasir, I., Benton, B. K., Kladde, M. P., Laurent, B. C. (1998). Sth1p, a Saccharomyces cerevisiae Snf2p/Swi2p Homolog, Is an Essential ATPase in RSC and Differs From Snf/Swi in Its Interactions With Histones and Chromatin-Associated Proteins. Genetics 150: 987-1005 [Abstract] [Full Text]  
• Friesen, H., Tanny, J. C., Segall, J. (1998). SPE3, Which Encodes Spermidine Synthase, Is Required for Full Repression Through NREDIT in Saccharomyces cerevisiae. Genetics 150: 59-73 [Abstract] [Full Text]  
• Brewster, N. K., Johnston, G. C., Singer, R. A. (1998). Characterization of the CP Complex, an Abundant Dimer of Cdc68 and Pob3 Proteins That Regulates Yeast Transcriptional Activation and Chromatin Repression. J. Biol. Chem. 273: 21972-21979 [Abstract] [Full Text]  
• Hampsey, M. (1998). Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery. Microbiol. Mol. Biol. Rev. 62: 465-503 [Abstract] [Full Text]  
• Hartzog, G. A., Wada, T., Handa, H., Winston, F. (1998). Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. Genes Dev. 12: 357-369 [Abstract] [Full Text]  
• DeSilva, H., Lee, K., Osley, M. A. (1998). Functional Dissection of Yeast Hir1p, a WD Repeat–Containing Transcriptional Corepressor. Genetics 148: 657-668 [Abstract] [Full Text]  
• Laurent, B C, Treich, I, Carlson, M (1993). The yeast SNF2/SWI2 protein has DNA-stimulated ATPase activity required for transcriptional activation.. Genes Dev. 7: 583-591 [Abstract]  
• Laurent, B C, Carlson, M (1992). Yeast SNF2/SWI2, SNF5, and SNF6 proteins function coordinately with the gene-specific transcriptional activators GAL4 and Bicoid.. Genes Dev. 6: 1707-1715 [Abstract]  
• Clark-Adams, C D, Norris, D, Osley, M A, Fassler, J S, Winston, F (1988). Changes in histone gene dosage alter transcription in yeast.. Genes Dev. 2: 150-159 [Abstract]