This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chabot, B Articles by Steitz, J A Search for Related Content PubMed PubMed Citation Articles by Chabot, B Articles by Steitz, J A

 Previous Article  |  Next Article 

Mol Cell Biol. 1987 February; 7(2): 698-707

Recognition of mutant and cryptic 5' splice sites by the U1 small nuclear ribonucleoprotein in vitro.

ABSTRACT

We examined the ability of U1 small nuclear ribonucleoproteins (U1 snRNPs) to recognize mutant and cryptic 5' splice sites on beta-globin pre-mRNA substrates using an RNase T1 protection assay. When U1 snRNPs were prebound to anti-(U1)RNP antibodies, we detected binding to mutant but not to cryptic 5' splice sites on several substrates. By contrast, in a splicing extract at 0 degree C, neither the mutated nor cryptic 5' splice sites of a human beta-globin transcript were selected as protected fragments with the same antibodies. However, after incubation of the transcript in the extract to yield splicing intermediates, fragments that included a cryptic 5' splice site were detected. The results of our analyses suggest that U1 snRNPs are involved in recognizing cryptic 5' splice sites but that interactions with other splicing components are required to stabilize the association.

This article has been cited by other articles:

• ROCA, X., SACHIDANANDAM, R., KRAINER, A. R. (2005). Determinants of the inherent strength of human 5' splice sites. RNA 11: 683-698 [Abstract] [Full Text]  
• Roca, X., Sachidanandam, R., Krainer, A. R. (2003). Intrinsic differences between authentic and cryptic 5' splice sites. Nucleic Acids Res 31: 6321-6333 [Abstract] [Full Text]  
• Eperon, I. C., Makarova, O. V., Mayeda, A., Munroe, S. H., Cáceres, J. F., Hayward, D. G., Krainer, A. R. (2000). Selection of Alternative 5' Splice Sites: Role of U1 snRNP and Models for the Antagonistic Effects of SF2/ASF and hnRNP A1. Mol. Cell. Biol. 20: 8303-8318 [Abstract] [Full Text]  
• O'Mullane, L., Eperon, I. C. (1998). The Pre-mRNA 5' Cap Determines Whether U6 Small Nuclear RNA Succeeds U1 Small Nuclear Ribonucleoprotein Particle at 5' Splice Sites. Mol. Cell. Biol. 18: 7510-7520 [Abstract] [Full Text]  
• Lavigueur, A, La Branche, H, Kornblihtt, A R, Chabot, B (1993). A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding.. Genes Dev. 7: 2405-2417 [Abstract]  
• Siebel, C W, Fresco, L D, Rio, D C (1992). The mechanism of somatic inhibition of Drosophila P-element pre-mRNA splicing: multiprotein complexes at an exon pseudo-5' splice site control U1 snRNP binding.. Genes Dev. 6: 1386-1401 [Abstract]  
• Black, D L (1991). Does steric interference between splice sites block the splicing of a short c-src neuron-specific exon in non-neuronal cells?. Genes Dev. 5: 389-402 [Abstract]  
• Siebel, C., Rio, D. (1990). Regulated splicing of the Drosophila P transposable element third intron in vitro: somatic repression. Science 248: 1200-1208 [Abstract]  
• Kedes, D H, Steitz, J A (1988). Correct in vivo splicing of the mouse immunoglobulin kappa light-chain pre-mRNA is dependent on 5' splice-site position even in the absence of transcription.. Genes Dev. 2: 1448-1459 [Abstract]  
• Nelson, K K, Green, M R (1988). Splice site selection and ribonucleoprotein complex assembly during in vitro pre-mRNA splicing.. Genes Dev. 2: 319-329 [Abstract]