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Mol Cell Biol. 1987 February; 7(2): 821-829

Different functional domains of the adenovirus E1A gene are involved in regulation of host cell cycle products.

ABSTRACT

We have analyzed the cell cycle effects that different domains of the adenovirus E1A proteins have on quiescent primary BRK cells. Studies with deletion mutants that in combination removed all but the N-terminal 85 amino acids common to both the 12S and 13S proteins suggest that this region may be sufficient for the induction of synthesis of proliferating cell nuclear antigen and the stimulation of DNA synthesis. A second domain also common to the N-terminal exon of the 12S and 13S proteins was required for the induction of mitosis and stimulation of proliferation of primary BRK cells. A virus containing a mutation in this region was still able to stimulate DNA synthesis efficiently. A third domain, unique to the 13S protein, was required for the accelerated activation of the cellular thymidylate synthase gene in a manner similar to the 13S-dependent stimulation of adenovirus early region genes.

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• Hu, P. P.-c., Datto, M. B., Wang, X.-f. (1998). Molecular Mechanisms of Transforming Growth Factor-{beta} Signaling. Endocr. Rev. 19: 349-363 [Abstract] [Full Text]  
• Fischer, R. S., Quinlan, M. P. (1998). Expression of the pRb-Binding Regions of E1A Enables Efficient Transformation of Primary Epithelial Cells by v-src. J. Virol. 72: 2815-2824 [Abstract] [Full Text]  
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• Lee, J.-S., Zhang, X., Shi, Y. (1996). Differential Interactions of the CREB/ATF Family of Transcription Factors with p300 and Adenovirus E1A. J. Biol. Chem. 271: 17666-17674 [Abstract] [Full Text]  
• Eckner, R, Ewen, M E, Newsome, D, Gerdes, M, DeCaprio, J A, Lawrence, J B, Livingston, D M (1994). Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.. Genes Dev. 8: 869-884 [Abstract]  
• Giordano, A, Lee, J., Scheppler, J., Herrmann, C, Harlow, E, Deuschle, U, Beach, D, Franza, B. Jr (1991). Cell cycle regulation of histone H1 kinase activity associated with the adenoviral protein E1A. Science 253: 1271-1275 [Abstract]  
• Raychaudhuri, P, Bagchi, S, Devoto, S H, Kraus, V B, Moran, E, Nevins, J R (1991). Domains of the adenovirus E1A protein required for oncogenic activity are also required for dissociation of E2F transcription factor complexes.. Genes Dev. 5: 1200-1211 [Abstract]  
• Dyson, N, Howley, P., Munger, K, Harlow, E (1989). The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science 243: 934-937 [Abstract]