This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Speck, N A Articles by Baltimore, D Search for Related Content PubMed PubMed Citation Articles by Speck, N A Articles by Baltimore, D

Six distinct nuclear factors interact with the 75-base-pair repeat of the Moloney murine leukemia virus enhancer.

ABSTRACT

Binding sites for six distinct nuclear factors on the 75-base-pair repeat of the Moloney murine leukemia virus enhancer have been identified by an electrophoretic mobility shift assay combined with methylation interference. Three of these factors, found in WEHI 231 nuclear extracts, which we have named LVa, LVb, and LVc (for leukemia virus factors a, b, and c) have not been previously identified. Nuclear factors that bind to the conserved simian virus 40 corelike motif, the NF-1 motif, and the glucocorticoid response element were also detected. Testing of multiple cell lines showed that most factors appeared ubiquitous, except that the NF-1 binding factor was found neither in nuclear extracts from MEL cells nor in the embryonal carcinoma cell lines PCC4 and F9, and core-binding factor was relatively depleted from MEL and F9 nuclear extracts.

This article has been cited by other articles:

• Voisin, V., Barat, C., Hoang, T., Rassart, E. (2006). Novel insights into the pathogenesis of the graffi murine leukemia retrovirus.. J. Virol. 80: 4026-4037 [Abstract] [Full Text]  
• Hronek, B. W., Meagher, A., Rovnak, J., Quackenbush, S. L. (2004). Identification and Characterization of cis-Acting Elements Residing in the Walleye Dermal Sarcoma Virus Promoter. J. Virol. 78: 7590-7601 [Abstract] [Full Text]  
• Haas, D. L., Lutzko, C., Logan, A. C., Cho, G. J., Skelton, D., Jin Yu, X., Pepper, K. A., Kohn, D. B. (2003). The Moloney Murine Leukemia Virus Repressor Binding Site Represses Expression in Murine and Human Hematopoietic Stem Cells. J. Virol. 77: 9439-9450 [Abstract] [Full Text]  
• Wilson, C. A., Laeeq, S., Ritzhaupt, A., Colon-Moran, W., Yoshimura, F. K. (2002). Sequence Analysis of Porcine Endogenous Retrovirus Long Terminal Repeats and Identification of Transcriptional Regulatory Regions. J. Virol. 77: 142-149 [Abstract] [Full Text]  
• Wahlers, A., Zipfel, P. F., Schwieger, M., Ostertag, W., Baum, C. (2002). In Vivo Analysis of Retroviral Enhancer Mutations in Hematopoietic Cells: SP1/EGR1 and ETS/GATA Motifs Contribute to Long Terminal Repeat Specificity. J. Virol. 76: 303-312 [Abstract] [Full Text]  
• Kowolik, C. M., Hu, J., Yee, J.-K. (2001). Locus Control Region of the Human CD2 Gene in a Lentivirus Vector Confers Position-Independent Transgene Expression. J. Virol. 75: 4641-4648 [Abstract] [Full Text]  
• Mertz, J. A., Mustafa, F., Meyers, S., Dudley, J. P. (2001). Type B Leukemogenic Virus Has a T-Cell-Specific Enhancer That Binds AML-1. J. Virol. 75: 2174-2184 [Abstract] [Full Text]  
• Modin, C., Pedersen, F. S., Duch, M. (2000). Lack of Shielding of Primer Binding Site Silencer-Mediated Repression of an Internal Promoter in a Retrovirus Vector by the Putative Insulators scs, BEAD-1, and HS4. J. Virol. 74: 11697-11707 [Abstract] [Full Text]  
• Hamaguchi, I., Woods, N.-B., Panagopoulos, I., Andersson, E., Mikkola, H., Fahlman, C., Zufferey, R., Carlsson, L., Trono, D., Karlsson, S. (2000). Lentivirus Vector Gene Expression during ES Cell-Derived Hematopoietic Development In Vitro. J. Virol. 74: 10778-10784 [Abstract] [Full Text]  
• Gu, T.-L., Goetz, T. L., Graves, B. J., Speck, N. A. (2000). Auto-Inhibition and Partner Proteins, Core-Binding Factor beta (CBFbeta ) and Ets-1, Modulate DNA Binding by CBFalpha 2 (AML1). Mol. Cell. Biol. 20: 91-103 [Abstract] [Full Text]  
• Jäger, U., Zhao, Y., Porter, C. D. (1999). Endothelial Cell-Specific Transcriptional Targeting from a Hybrid Long Terminal Repeat Retrovirus Vector Containing Human Prepro-Endothelin-1 Promoter Sequences. J. Virol. 73: 9702-9709 [Abstract] [Full Text]  
• Le, X.-F., Groner, Y., Kornblau, S. M., Gu, Y., Hittelman, W. N., Levanon, D., Mehta, K., Arlinghaus, R. B., Chang, K.-S. (1999). Regulation of AML2/CBFA3 in Hematopoietic Cells through the Retinoic Acid Receptor alpha -Dependent Signaling Pathway. J. Biol. Chem. 274: 21651-21658 [Abstract] [Full Text]  
• Osborne, C. S., Pasceri, P., Singal, R., Sukonnik, T., Ginder, G. D., Ellis, J. (1999). Amelioration of Retroviral Vector Silencing in Locus Control Region beta -Globin-Transgenic Mice and Transduced F9 Embryonic Cells. J. Virol. 73: 5490-5496 [Abstract] [Full Text]  
• Lewis, A. F., Stacy, T., Green, W. R., Taddesse-Heath, L., Hartley, J. W., Speck, N. A. (1999). Core-Binding Factor Influences the Disease Specificity of Moloney Murine Leukemia Virus. J. Virol. 73: 5535-5547 [Abstract] [Full Text]  
• Martiney, M. J., Levy, L. S., Lenz, J. (1999). Suppressor Mutations within the Core Binding Factor (CBF/AML1) Binding Site of a T-Cell Lymphomagenic Retrovirus. J. Virol. 73: 2143-2152 [Abstract] [Full Text]  
• DiFronzo, N. L., Holland, C. A. (1999). Sequence-Specific and/or Stereospecific Constraints of the U3 Enhancer Elements of MCF 247-W Are Important for Pathogenicity. J. Virol. 73: 234-241 [Abstract] [Full Text]  
• Lee, S.-Y., Howard, T. M., Rasheed, S. (1998). Genetic Analysis of the Rat Leukemia Virus: Influence of Viral Sequences in Transduction of the c-ras Proto-Oncogene and Expression of Its Transforming Activity. J. Virol. 72: 9906-9917 [Abstract] [Full Text]  
• Granger, S. W., Fan, H. (1998). In Vivo Footprinting of the Enhancer Sequences in the Upstream Long Terminal Repeat of Moloney Murine Leukemia Virus: Differential Binding of Nuclear Factors in Different Cell Types. J. Virol. 72: 8961-8970 [Abstract] [Full Text]  
• Chen, H., Bechtel, M. K., Shi, Y., Phipps, A., Mathes, L. E., Hayes, K. A., Roy-Burman, P. (1998). Pathogenicity Induced by Feline Leukemia Virus, Rickard Strain, Subgroup A Plasmid DNA (pFRA). J. Virol. 72: 7048-7056 [Abstract] [Full Text]  
• Zaiman, A. L., Nieves, A., Lenz, J. (1998). CBF, Myb, and Ets Binding Sites Are Important for Activity of the Core I Element of the Murine Retrovirus SL3-3 in T Lymphocytes. J. Virol. 72: 3129-3137 [Abstract] [Full Text]  
• Starkey, C. R., Lobelle-Rich, P. A., Granger, S., Brightman, B. K., Fan, H., Levy, L. S. (1998). Tumorigenic Potential of a Recombinant Retrovirus Containing Sequences from Moloney Murine Leukemia Virus and Feline Leukemia Virus. J. Virol. 72: 1078-1084 [Abstract] [Full Text]  
• Cervini, R., Houhou, L.ïl., Pradat, P.-F.ço., Béjanin, S.ép., Mallet, J., Berrard, S. (1995). Specific Vesicular Acetylcholine Transporter Promoters Lie within the First Intron of the Rat Choline Acetyltransferase Gene. J. Biol. Chem. 270: 24654-24657 [Abstract] [Full Text]  
• Liu, P, Tarle, S., Hajra, A, Claxton, D., Marlton, P, Freedman, M, Siciliano, M., Collins, F. (1993). Fusion between transcription factor CBF beta/PEBP2 beta and a myosin heavy chain in acute myeloid leukemia. Science 261: 1041-1044 [Abstract]  
• Oshima, R G, Abrams, L, Kulesh, D (1990). Activation of an intron enhancer within the keratin 18 gene by expression of c-fos and c-jun in undifferentiated F9 embryonal carcinoma cells.. Genes Dev. 4: 835-848 [Abstract]  
• Speck, N A, Renjifo, B, Golemis, E, Fredrickson, T N, Hartley, J W, Hopkins, N (1990). Mutation of the core or adjacent LVb elements of the Moloney murine leukemia virus enhancer alters disease specificity.. Genes Dev. 4: 233-242 [Abstract]  
• Schule, R, Muller, M, Kaltschmidt, C, Renkawitz, R (1988). Many transcription factors interact synergistically with steroid receptors. Science 242: 1418-1420 [Abstract]  
• Williams, T, Admon, A, Luscher, B, Tjian, R (1988). Cloning and expression of AP-2, a cell-type-specific transcription factor that activates inducible enhancer elements.. Genes Dev. 2: 1557-1569 [Abstract]  
• Landschulz, W H, Johnson, P F, Adashi, E Y, Graves, B J, McKnight, S L (1988). Isolation of a recombinant copy of the gene encoding C/EBP.. Genes Dev. 2: 786-800 [Abstract]  
• Varmus, H (1988). Retroviruses. Science 240: 1427-1435 [Abstract]