This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stein, R W Articles by Ziff, E B Search for Related Content PubMed PubMed Citation Articles by Stein, R W Articles by Ziff, E B

 Previous Article  |  Next Article 

Mol Cell Biol. 1987 March; 7(3): 1164-1170

Repression of insulin gene expression by adenovirus type 5 E1a proteins.

ABSTRACT

Insulin gene transcription relies on enhancer and promoter elements which are active in pancreatic beta cells. We showed that adenovirus type 5 infection of HIT T-15 cells, a transformed hamster beta cell line, represses insulin gene transcription and mRNA levels. Using expression plasmids transiently introduced into HIT T-15 cells, we showed that adenovirus type 5 E1a transcription regulatory proteins repress insulin enhancer-promoter element activity as assayed with a surrogate xanthine-guanine phosphoribosyltransferase gene. We relate E1a repression of the insulin gene to other examples of repression of enhancer-dependent genes by E1a and discuss the possible relationship of this repression to insulin gene regulation.

This article has been cited by other articles:

• Behzad, A. R., Morimoto, K., Gosselink, J., Green, J., Hogg, J. C., Hayashi, S. (2006). Induction of mesenchymal cell phenotypes in lung epithelial cells by adenovirus E1A. Eur Respir J 28: 1106-1117 [Abstract] [Full Text]  
• Fang, L., Spindler, K. R. (2005). E1A-CR3 Interaction-Dependent and -Independent Functions of mSur2 in Viral Replication of Early Region 1A Mutants of Mouse Adenovirus Type 1. J. Virol. 79: 3267-3276 [Abstract] [Full Text]  
• Boyd, J. M., Loewenstein, P. M., Tang, Q.-q., Yu, L., Green, M. (2002). Adenovirus E1A N-Terminal Amino Acid Sequence Requirements for Repression of Transcription In Vitro and In Vivo Correlate with Those Required for E1A Interference with TBP-TATA Complex Formation. J. Virol. 76: 1461-1474 [Abstract] [Full Text]  
• Lee, W.-P., Liao, Y., Robinson, D., Kung, H.-J., Liu, E. T., Hung, M.-C. (1999). Axl-Gas6 Interaction Counteracts E1A-Mediated Cell Growth Suppression and Proapoptotic Activity. Mol. Cell. Biol. 19: 8075-8082 [Abstract] [Full Text]  
• Ying, B., Smith, K., Spindler, K. R. (1998). Mouse Adenovirus Type 1 Early Region 1A Is Dispensable for Growth in Cultured Fibroblasts. J. Virol. 72: 6325-6331 [Abstract] [Full Text]  
• Smith, K., Brown, C. C., Spindler, K. R. (1998). The Role of Mouse Adenovirus Type 1 Early Region 1A in Acute and Persistent Infections in Mice. J. Virol. 72: 5699-5706 [Abstract] [Full Text]  
• Bishopric, N. H., Zeng, G.-Q., Sato, B., Webster, K. A. (1997). Adenovirus E1A Inhibits Cardiac Myocyte-specific Gene Expression through Its Amino Terminus. J. Biol. Chem. 272: 20584-20594 [Abstract] [Full Text]  
• Lee, J.-S., Zhang, X., Shi, Y. (1996). Differential Interactions of the CREB/ATF Family of Transcription Factors with p300 and Adenovirus E1A. J. Biol. Chem. 271: 17666-17674 [Abstract] [Full Text]  
• Song, C.-Z., Tierney, C. J., Loewenstein, P. M., Pusztai, R., Symington, J. S., Tang, Q.-q., Toth, K., Nishikawa, A., Bayley, S. T., Green, M. (1995). Transcriptional Repression by Human Adenovirus E1A N Terminus/Conserved Domain 1 Polypeptides in Vivo and in Vitro in the Absence of Protein Synthesis. J. Biol. Chem. 270: 23263-23267 [Abstract] [Full Text]  
• Eckner, R, Ewen, M E, Newsome, D, Gerdes, M, DeCaprio, J A, Lawrence, J B, Livingston, D M (1994). Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.. Genes Dev. 8: 869-884 [Abstract]  
• Braun, T, Bober, E, Arnold, H H (1992). Inhibition of muscle differentiation by the adenovirus E1a protein: repression of the transcriptional activating function of the HLH protein Myf-5.. Genes Dev. 6: 888-902 [Abstract]  
• Rochette-Egly, C, Fromental, C, Chambon, P (1990). General repression of enhanson activity by the adenovirus-2 E1A proteins.. Genes Dev. 4: 137-150 [Abstract]  
• Muller, U, Roberts, M P, Engel, D A, Doerfler, W, Shenk, T (1989). Induction of transcription factor AP-1 by adenovirus E1A protein and cAMP.. Genes Dev. 3: 1991-2002 [Abstract]  
• Engel, D A, Hardy, S, Shenk, T (1988). cAMP acts in synergy with E1A protein to activate transcription of the adenovirus early genes E4 and E1A.. Genes Dev. 2: 1517-1528 [Abstract]  
• Hurst, H C, Jones, N C (1987). Identification of factors that interact with the E1A-inducible adenovirus E3 promoter.. Genes Dev. 1: 1132-1146 [Abstract]  
• Yoshida, K, Venkatesh, L, Kuppuswamy, M, Chinnadurai, G (1987). Adenovirus transforming 19-kD T antigen has an enhancer-dependent trans-activation function and relieves enhancer repression mediated by viral and cellular genes.. Genes Dev. 1: 645-658 [Abstract]  
• Maniatis, T, Goodbourn, S, Fischer, J. (1987). Regulation of inducible and tissue-specific gene expression. Science 236: 1237-1245 [Abstract]  
• Lu, W., Peterson, R., Dasgupta, A., Scovell, W. M. (2000). Influence of HMG-1 and Adenovirus Oncoprotein E1A on Early Stages of Transcriptional Preinitiation Complex Assembly. J. Biol. Chem. 275: 35006-35012 [Abstract] [Full Text]