This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by West, B L Articles by Reudelhuber, T L Search for Related Content PubMed PubMed Citation Articles by West, B L Articles by Reudelhuber, T L

Interaction of a tissue-specific factor with an essential rat growth hormone gene promoter element.

ABSTRACT

Rat growth hormone (rGH) gene expression is normally restricted to the anterior pituitary. As a model of this tissue specificity, we compared the transient expression of an rGH-chloramphenicol acetyltransferase (CAT) hybrid gene in rGH-producing rat pituitary tumor (GC) cells and in non-rGH-producing rat fibroblast (rat-2) cells. Deletion analysis of the rGH portion of this hybrid gene demonstrated that DNA sequences within 140 base pairs 5' to the rGH gene were sufficient for correct cell type-specific expression. Deletion of an additional 35 base pairs of the rGH 5'-flanking DNA resulted in a loss of expression of the transfected hybrid gene and correlated with the interaction of a putative trans-acting factor with this region of the rGH promoter. This factor was detectable by DNase I footprinting in a crude nuclear extract from GC cells but not from rat-2 cells. Site-directed mutagenesis of the footprint region caused complete loss of expression of a hybrid gene containing 530 base pairs 5' to the rGH gene. Thus, the interaction of this factor, which we term GC2, is likely to be essential for the tissue-specific expression of the rGH gene.

This article has been cited by other articles:

• Schaufele, F., Schaufele, F. (1996). CCAAT/Enhancer-binding Protein alpha Activation of the Rat Growth Hormone Promoter in Pituitary Progenitor GHFT1-5 Cells. J. Biol. Chem. 271: 21484-21489 [Abstract] [Full Text]  
• Garami, M., Gardner, D. G. (1996). An E-Box Motif Conveys Inhibitory Activity on the Atrial Natriuretic Peptide Gene. Hypertension 28: 315-319 [Abstract] [Full Text]  
• Chang, W., Zhou, W., Theill, L. E., Baxter, J. D., Schaufele, F. (1996). An Activation Function in Pit-1 Required Selectively for Synergistic Transcription. J. Biol. Chem. 271: 17733-17738 [Abstract] [Full Text]  
• Smith, K. P., Liu, B., Scott, C., Sharp, Z. D. (1995). Pit-1 Exhibits a Unique Promoter Spacing Requirement for Activation and Synergism. J. Biol. Chem. 270: 4484-4491 [Abstract] [Full Text]  
• Drolet, D W, Scully, K M, Simmons, D M, Wegner, M, Chu, K T, Swanson, L W, Rosenfeld, M G (1991). TEF, a transcription factor expressed specifically in the anterior pituitary during embryogenesis, defines a new class of leucine zipper proteins.. Genes Dev. 5: 1739-1753 [Abstract]  
• Simmons, D M, Voss, J W, Ingraham, H A, Holloway, J M, Broide, R S, Rosenfeld, M G, Swanson, L W (1990). Pituitary cell phenotypes involve cell-specific Pit-1 mRNA translation and synergistic interactions with other classes of transcription factors.. Genes Dev. 4: 695-711 [Abstract]  
• Mangalam, H J, Albert, V R, Ingraham, H A, Kapiloff, M, Wilson, L, Nelson, C, Elsholtz, H, Rosenfeld, M G (1989). A pituitary POU domain protein, Pit-1, activates both growth hormone and prolactin promoters transcriptionally.. Genes Dev. 3: 946-958 [Abstract]  
• Crenshaw, E B, Kalla, K, Simmons, D M, Swanson, L W, Rosenfeld, M G (1989). Cell-specific expression of the prolactin gene in transgenic mice is controlled by synergistic interactions between promoter and enhancer elements.. Genes Dev. 3: 959-972 [Abstract]  
• Tripputi, P, Guerin, S., Moore, D. (1988). Two mechanisms for the extinction of gene expression in hybrid cells. Science 241: 1205-1207 [Abstract]  
• Behringer, R R, Mathews, L S, Palmiter, R D, Brinster, R L (1988). Dwarf mice produced by genetic ablation of growth hormone-expressing cells.. Genes Dev. 2: 453-461 [Abstract]  
• Nelson, C, Albert, V., Elsholtz, H., Lu, L., Rosenfeld, M. (1988). Activation of cell-specific expression of rat growth hormone and prolactin genes by a common transcription factor. Science 239: 1400-1405 [Abstract]