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Mol Cell Biol. 1987 March; 7(3): 1276-1279

Episomal maintenance of a bovine papilloma virus vector in transgenic mice.

A Elbrecht, F J DeMayo, M J Tsai and B W O'Malley

ABSTRACT

We have used a bovine papillomavirus-based vector to generate transgenic mice. Transgenic mice result from either pronuclear or cytoplasmic injections of the vector into fertilized eggs. Of 30 mice generated by microinjection, 27 (90%) contained the vector in its episomal form, at less than one copy per cell. This represents a highly efficient means of gene transfer in which the transgene is in a controlled genetic environment.

Mol Cell Biol. 1987 March; 7(3): 1276-1279




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Copyright © 1987 by the American Society for Microbiology. All rights reserved.