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Mol Cell Biol. 1987 April; 7(4): 1346-1351

In vitro splicing of kappa immunoglobulin precursor mRNA.

ABSTRACT

The in vitro splicing of kappa immunoglobulin precursor mRNA was studied as an example of a naturally occurring mRNA possessing multiple 5' splice sites. Several kappa mRNAs were generated in vitro by using an SP6 transcription system and were spliced in nuclear extracts derived from HeLa cells. Products and intermediates resulting from in vitro splicing were identified and characterized. In contrast to the in vivo situation, in which apparently only the 5'-most splice donor site is used, all of the 5' splice sites were used in vitro with equal frequency. Neither the presence or absence of variable region coding sequences nor the deletion of intron sequences had an effect on in vitro splice site selection.

This article has been cited by other articles:

• Kedes, D H, Steitz, J A (1988). Correct in vivo splicing of the mouse immunoglobulin kappa light-chain pre-mRNA is dependent on 5' splice-site position even in the absence of transcription.. Genes Dev. 2: 1448-1459 [Abstract]  
• Beyer, A L, Osheim, Y N (1988). Splice site selection, rate of splicing, and alternative splicing on nascent transcripts.. Genes Dev. 2: 754-765 [Abstract]