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Both conserved signals on mammalian histone pre-mRNAs associate with small nuclear ribonucleoproteins during 3' end formation in vitro.

ABSTRACT

Pre-mRNA substrates containing sequences from human and mouse histone genes are accurately processed in a HeLa cell nuclear extract to generate mature 3' termini. When in vitro processing reactions containing either human histone H3 or mouse histone H3 transcripts are treated with RNase T1 and probed with antibodies specific for the Sm protein determinants or for the trimethylguanosine cap structure unique to the U RNAs present in small nuclear ribonucleoproteins, RNA fragments that encompass the site of 3' end formation on the pre-mRNA transcript are selectively recovered. Several different interactions are detected: at time zero, the protected region contains the upstream conserved hairpin loop structure; at later times during the reaction, protection extends beyond the site of 3' end formation to include the downstream conserved sequence element and the 5' cap of the transcript is bound as well. Possible interactions between Sm small nuclear ribonucleoproteins and these conserved sequence elements in histone pre-mRNAs are discussed.

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• Dominski, Z., Zheng, L.-X., Sanchez, R., Marzluff, W. F. (1999). Stem-Loop Binding Protein Facilitates 3'-End Formation by Stabilizing U7 snRNP Binding to Histone Pre-mRNA. Mol. Cell. Biol. 19: 3561-3570 [Abstract] [Full Text]  
• Abbott, J., Marzluff, W. F., Gall, J. G. (1999). The Stem-Loop Binding Protein (SLBP1) Is Present in Coiled Bodies of the Xenopus Germinal Vesicle. Mol. Biol. Cell 10: 487-499 [Abstract] [Full Text]  
• Mowry, K. L. (1996). Complex formation between stage-specific oocyte factors and a Xenopus mRNA localization element. Proc. Natl. Acad. Sci. USA 93: 14608-14613 [Abstract] [Full Text]  
• Scharl, E. C., Steitz, J. A. (1996). Length suppression in histone messenger RNA 3'-end maturation: Processing defects of insertion mutant premessenger RNAs can be compensated by insertions into the U7 small nuclear RNA. Proc. Natl. Acad. Sci. USA 93: 14659-14664 [Abstract] [Full Text]  
• Wang, Z F, Whitfield, M L, Ingledue, T C, Dominski, Z, Marzluff, W F (1996). The protein that binds the 3' end of histone mRNA: a novel RNA-binding protein required for histone pre-mRNA processing.. Genes Dev. 10: 3028-3040 [Abstract]  
• Wassarman, K M, Steitz, J A (1993). Association with terminal exons in pre-mRNAs: a new role for the U1 snRNP?. Genes Dev. 7: 647-659 [Abstract]  
• Bond, U M, Yario, T A, Steitz, J A (1991). Multiple processing-defective mutations in a mammalian histone pre-mRNA are suppressed by compensatory changes in U7 RNA both in vivo and in vitro.. Genes Dev. 5: 1709-1722 [Abstract]  
• Kirsh, A L, Groudine, M, Challoner, P B (1989). Polyadenylation and U7 snRNP-mediated cleavage: alternative modes of RNA 3' processing in two avian histone H1 genes.. Genes Dev. 3: 2172-2179 [Abstract]  
• Gilmartin, G M, McDevitt, M A, Nevins, J R (1988). Multiple factors are required for specific RNA cleavage at a poly(A) addition site.. Genes Dev. 2: 578-587 [Abstract]  
• Mowry, K., Steitz, J. (1987). Identification of the human U7 snRNP as one of several factors involved in the 3' end maturation of histone premessenger RNA's. Science 238: 1682-1687 [Abstract]  
• Muller, B., Link, J., Smythe, C. (2000). Assembly of U7 Small Nuclear Ribonucleoprotein Particle and Histone RNA 3' Processing in Xenopus Egg Extracts. J. Biol. Chem. 275: 24284-24293 [Abstract] [Full Text]