This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Samid, D Articles by Friedman, R M Search for Related Content PubMed PubMed Citation Articles by Samid, D Articles by Friedman, R M

 Previous Article  |  Next Article 

Mol Cell Biol. 1987 June; 7(6): 2196-2200

Interferon-induced revertants of ras-transformed cells: resistance to transformation by specific oncogenes and retransformation by 5-azacytidine.

ABSTRACT

Prolonged alpha/beta interferon (IFN-alpha/beta) treatment of NIH 3T3 cells transformed by a long terminal repeat-activated Ha-ras proto-oncogene resulted in revertants that maintained a nontransformed phenotype long after IFN treatment had been discontinued. Cloned persistent revertants (PRs) produced large amounts of the ras-encoded p21 and were refractile to transformation by EJras DNA and by transforming retroviruses which carried the v-Ha-ras, v-Ki-ras, v-abl, or v-fes oncogene. Transient treatment either in vitro or in vivo with cytidine analogs that alter gene expression by inhibiting DNA methylation resulted in transformation of PR, but not of NIH 3T3, cells. The PR retransformants reverted again with IFN, suggesting that DNA methylation is involved in IFN-induced persistent reversion.

This article has been cited by other articles:

• Palamakumbura, A. H., Jeay, S., Guo, Y., Pischon, N., Sommer, P., Sonenshein, G. E., Trackman, P. C. (2004). The Propeptide Domain of Lysyl Oxidase Induces Phenotypic Reversion of Ras-transformed Cells. J. Biol. Chem. 279: 40593-40600 [Abstract] [Full Text]  
• Palamakumbura, A. H., Sommer, P., Trackman, P. C. (2003). Autocrine Growth Factor Regulation of Lysyl Oxidase Expression in Transformed Fibroblasts. J. Biol. Chem. 278: 30781-30787 [Abstract] [Full Text]  
• Hannigan, G., Williams, B. (1991). Signal transduction by interferon-alpha through arachidonic acid metabolism. Science 251: 204-207 [Abstract]  
• Contente, S, Kenyon, K, Rimoldi, D, Friedman, R. (1990). Expression of gene rrg is associated with reversion of NIH 3T3 transformed by LTR-c-H-ras. Science 249: 796-798 [Abstract]