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Mol Cell Biol. 1987 July; 7(7): 2506-2511

Molecular cloning and characterization of the cys-3 regulatory gene of Neurospora crassa.

ABSTRACT

The regulatory gene cys-3+ controls the synthesis of a number of enzymes involved in sulfur metabolism. cys-3 mutants show a multiple loss of enzymes in different pathways of sulfur metabolism. The cys-3+ gene was isolated by transformation of an aro-9 qa-2 cys-3 inl strain with a clone bank followed by screening with the "sib selection" method. The library used (pRAL1) contained inserts of Sau3a partial digest fragments of about 9 kilobases as well as the Neurospora qa-2+ gene. Double selection for qa-2+ and cys-3+ function was carried out. The transformants obtained with the isolated cys-3+ clone show recovery of the enzyme activities associated with the cys-3 mutation (e.g., arylsulfatase and sulfate permease). Restriction fragment length polymorphism experiments confirmed the identity of the clone, mRNA studies with Northern blots show that the expression of the cys-3+ gene is inducible. In contrast to cys-3+, the cys-3 (P22) mutant gene was not expressed at a higher level under sulfur-derepressed conditions.

This article has been cited by other articles:

• Kumar, A., Paietta, J. V. (1998). An additional role for the F-box motif: Gene regulation within the Neurospora crassa sulfur control network. Proc. Natl. Acad. Sci. USA 95: 2417-2422 [Abstract] [Full Text]  
• Tao, Y., Marzluf, G. A. (1998). Synthesis and Differential Turnover of the CYS3 Regulatory Protein of Neurospora crassa Are Subject to Sulfur Control. J. Bacteriol. 180: 478-482 [Abstract] [Full Text]