This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Colau, D Articles by Hernalsteens, J P Search for Related Content PubMed PubMed Citation Articles by Colau, D Articles by Hernalsteens, J P

 Previous Article  |  Next Article 

Mol Cell Biol. 1987 July; 7(7): 2552-2557

Complementation of a threonine dehydratase-deficient Nicotiana plumbaginifolia mutant after Agrobacterium tumefaciens-mediated transfer of the Saccharomyces cerevisiae ILV1 gene.

ABSTRACT

The Saccharomyces cerevisiae ILV1 gene, encoding threonine dehydratase (EC 4.2.1.16) was fused to the transferred DNA nopaline synthase promoter and the 3' noncoding region of the octopine synthase gene. It was introduced, by Agrobacterium tumefaciens-mediated gene transfer, into an isoleucine-requiring Nicotiana plumbaginifolia auxotroph deficient in threonine dehydratase. Functional complementation by the ILV1 gene product was demonstrated by the selection of several transformed lines on a medium without isoleucine and by the identification in these lines of the yeast threonine dehydratase activity. This is the first example illustrating the complementation of a plant auxotroph by transfection with a cloned gene.

This article has been cited by other articles:

• Chen, H., Gonzales-Vigil, E., Wilkerson, C. G., Howe, G. A. (2007). Stability of Plant Defense Proteins in the Gut of Insect Herbivores. Plant Physiol. 143: 1954-1967 [Abstract] [Full Text]  
• Kang, J.-H., Wang, L., Giri, A., Baldwin, I. T. (2006). Silencing Threonine Deaminase and JAR4 in Nicotiana attenuata Impairs Jasmonic Acid-Isoleucine-Mediated Defenses against Manduca sexta. Plant Cell 18: 3303-3320 [Abstract] [Full Text]  
• Huang, L., Brooks, S. A., Li, W., Fellers, J. P., Trick, H. N., Gill, B. S. (2003). Map-Based Cloning of Leaf Rust Resistance Gene Lr21 From the Large and Polyploid Genome of Bread Wheat. Genetics 164: 655-664 [Abstract] [Full Text]  
• Samuelsen, A. I., Martin, R. C., Mok, D. W.S., Mok, M. C. (1998). Expression of the Yeast FRE Genes in Transgenic Tobacco. Plant Physiol. 118: 51-58 [Abstract] [Full Text]