This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boggs, B
Right arrow Articles by Cabral, F
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boggs, B
Right arrow Articles by Cabral, F

 Previous Article  |  Next Article 

Mol Cell Biol. 1987 August; 7(8): 2700-2707

Mutations affecting assembly and stability of tubulin: evidence for a nonessential beta-tubulin in CHO cells.

B Boggs and F Cabral

Department of Medicine, University of Texas Medical School, Houston 77225.

ABSTRACT

Eight strains of Chinese hamster ovary (CHO) cells having an assembly-defective beta-tubulin were found among revertants of strain Cmd 4, a mutant with a conditional lethal mutation in a beta-tubulin gene (F. Cabral, M. E. Sobel, and M. M. Gottesman, Cell 20:29-36, 1980). The altered beta-tubulins in these strains have electrophoretically silent alterations or, in some cases, an increase or a decrease in apparent molecular weight based on their migration in two-dimensional gels. The identity of these variant proteins as beta-tubulin was confirmed by peptide mapping, which also revealed the loss of distinct methionine-containing peptides in the assembly-defective beta-tubulins of lower apparent molecular weight. The altered mobility of these beta-tubulin polypeptides was not the result of a posttranslational modification, since the altered species could be labeled in very short incubations with [35S]methionine and were found among in vitro-translated polypeptides by using purified mRNA. In at least one strain, an altered DNA restriction fragment could be demonstrated, suggesting that an alteration occurred in one of the structural genes for beta-tubulin. Assembly-defective beta-tubulin was unstable and turned over with a half-life of only 1 to 2 h in exponentially growing cells. This rapid degradation of a tubulin gene product resulted in approximately 30% lower steady-state levels of both alpha- and beta-tubulin yet did not affect the growth rate of the cells or the distribution of the microtubules as judged by immunofluorescence microscopy. These results argue that CHO cells possess a beta-tubulin gene product that is not essential for survival.

Mol Cell Biol. 1987 August; 7(8): 2700-2707




This article has been cited by other articles:

  • Yang, H., Cabral, F., Bhattacharya, R. (2009). Tubulin isotype specificity and identification of the epitope for antibody Tub 2.1. Protein Eng Des Sel 22: 625-629 [Abstract] [Full Text]  
  • Carr, H. S., Cai, C., Keinanen, K., Frost, J. A. (2009). Interaction of the RhoA Exchange Factor Net1 with Discs Large Homolog 1 Protects It from Proteasome-mediated Degradation and Potentiates Net1 Activity. J. Biol. Chem. 284: 24269-24280 [Abstract] [Full Text]  
  • Sellin, M. E., Holmfeldt, P., Stenmark, S., Gullberg, M. (2008). Global Regulation of the Interphase Microtubule System by Abundantly Expressed Op18/Stathmin. Mol. Biol. Cell 19: 2897-2906 [Abstract] [Full Text]  
  • Cheng, G., Zile, M. R., Takahashi, M., Baicu, C. F., Bonnema, D. D., Cabral, F., Menick, D. R., Cooper, G. 4th (2008). A direct test of the hypothesis that increased microtubule network density contributes to contractile dysfunction of the hypertrophied heart. Am. J. Physiol. Heart Circ. Physiol. 294: H2231-H2241 [Abstract] [Full Text]  
  • Yang, H., Cabral, F. (2007). Heightened Sensitivity to Paclitaxel in Class IVa beta-Tubulin-transfected Cells Is Lost as Expression Increases. J. Biol. Chem. 282: 27058-27066 [Abstract] [Full Text]  
  • Wang, Y., Tian, G., Cowan, N. J., Cabral, F. (2006). Mutations Affecting beta-Tubulin Folding and Degradation. J. Biol. Chem. 281: 13628-13635 [Abstract] [Full Text]  
  • Verrills, N. M., Walsh, B. J., Cobon, G. S., Hains, P. G., Kavallaris, M. (2003). Proteome Analysis of Vinca Alkaloid Response and Resistance in Acute Lymphoblastic Leukemia Reveals Novel Cytoskeletal Alterations. J. Biol. Chem. 278: 45082-45093 [Abstract] [Full Text]  
  • Hari, M., Wang, Y., Veeraraghavan, S., Cabral, F. (2002). Mutations in {alpha}- and {beta}-Tubulin That Stabilize Microtubules and Confer Resistance to Colcemid and Vinblastine. Molecular Cancer Therapeutics 2: 597-605 [Abstract] [Full Text]  
  • Barlow, S. B., Gonzalez-Garay, M. L., Cabral, F. (2002). Paclitaxel-dependent mutants have severely reduced microtubule assembly and reduced tubulin synthesis. J. Cell Sci. 115: 3469-3478 [Abstract] [Full Text]  
  • Gonzalez-Garay, M. L., Chang, L., Blade, K., Menick, D. R., Cabral, F. (1999). A beta -Tubulin Leucine Cluster Involved in Microtubule Assembly and Paclitaxel Resistance. J. Biol. Chem. 274: 23875-23882 [Abstract] [Full Text]  
  • Blade, K, Menick, D., Cabral, F (1999). Overexpression of class I, II or IVb beta-tubulin isotypes in CHO cells is insufficient to confer resistance to paclitaxel. J. Cell Sci. 112: 2213-2221 [Abstract]  
  • Yu, X., Margolin, W, Gonzalez-Garay, M., Cabral, F (1999). Vinblastine induces an interaction between FtsZ and tubulin in mammalian cells. J. Cell Sci. 112: 2301-2311 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»