This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mowatt, M R Articles by Clayton, C E Search for Related Content PubMed PubMed Citation Articles by Mowatt, M R Articles by Clayton, C E

 Previous Article  |  Next Article 

Mol Cell Biol. 1987 August; 7(8): 2838-2844

Developmental regulation of a novel repetitive protein of Trypanosoma brucei.

Rockefeller University, New York, New York 10021.

ABSTRACT

Trypanosoma brucei undergoes many morphological and biochemical changes during transformation from the bloodstream trypomastigote to the insect procyclic trypomastigote form. We cloned and determined the complete nucleotide sequence of a developmentally regulated cDNA. The corresponding mRNA was abundant in in vitro-cultivated procyclics but absent in bloodstream forms. The trypanosome genome contains eight genes homologous to this cDNA, arranged as four unlinked pairs of tandem repeats. The longest open reading frame of the cDNA predicts a protein of 15 kilodaltons, the central portion of which consists of 29 tandem glutamate-proline dipeptides. The repetitive region is preceded by an amino-terminal signal sequence and followed by a hydrophobic domain that could serve as a membrane anchor; the mRNA was found on membrane-bound polyribosomes. These results suggest that the protein is membrane associated.

This article has been cited by other articles:

• Manthri, S., Guther, M L. S, Izquierdo, L., Acosta-Serrano, A., Ferguson, M. A J (2008). Deletion of the TbALG3 gene demonstrates site-specific N-glycosylation and N-glycan processing in Trypanosoma brucei. Glycobiology 18: 367-383 [Abstract] [Full Text]  
• Guther, M. L. S., Lee, S., Tetley, L., Acosta-Serrano, A., Ferguson, M. A.J. (2006). GPI-anchored Proteins and Free GPI Glycolipids of Procyclic Form Trypanosoma brucei Are Nonessential for Growth, Are Required for Colonization of the Tsetse Fly, and Are Not the Only Components of the Surface Coat. Mol. Biol. Cell 17: 5265-5274 [Abstract] [Full Text]  
• Lustig, Y., Goldshmidt, H., Uliel, S., Michaeli, S. (2005). The Trypanosoma brucei signal recognition particle lacks the Alu-domain-binding proteins: purification and functional analysis of its binding proteins by RNAi. J. Cell Sci. 118: 4551-4562 [Abstract] [Full Text]  
• Roper, J. R., Guther, M. L. S., MacRae, J. I., Prescott, A. R., Hallyburton, I., Acosta-Serrano, A., Ferguson, M. A. J. (2005). The Suppression of Galactose Metabolism in Procylic Form Trypanosoma brucei Causes Cessation of Cell Growth and Alters Procyclin Glycoprotein Structure and Copy Number. J. Biol. Chem. 280: 19728-19736 [Abstract] [Full Text]  
• Yang, H., Russell, D. G., Zheng, B., Eiki, M., Lee, M. G.-S. (2000). Sequence Requirements for Trafficking of the CRAM Transmembrane Protein to the Flagellar Pocket of African Trypanosomes. Mol. Cell. Biol. 20: 5149-5163 [Abstract] [Full Text]  
• Tasker, M., Wilson, J., Sarkar, M., Hendriks, E., Matthews, K. (2000). A Novel Selection Regime for Differentiation Defects Demonstrates an Essential Role for the Stumpy Form in the Life Cycle of the African Trypanosome. Mol. Biol. Cell 11: 1905-1917 [Abstract] [Full Text]  
• Acosta-Serrano, A., Cole, R. N., Mehlert, A., Lee, M. G.-S., Ferguson, M. A. J., Englund, P. T. (1999). The Procyclin Repertoire of Trypanosoma brucei. IDENTIFICATION AND STRUCTURAL CHARACTERIZATION OF THE GLU-PRO-RICH POLYPEPTIDES. J. Biol. Chem. 274: 29763-29771 [Abstract] [Full Text]  
• Graham, S. V., Wymer, B., Barry, J. D. (1998). Activity of a Trypanosome Metacyclic Variant Surface Glycoprotein Gene Promoter Is Dependent upon Life Cycle Stage and Chromosomal Context. Mol. Cell. Biol. 18: 1137-1146 [Abstract] [Full Text]  
• Field, H., Field, M. C. (1997). Tandem Duplication of rab Genes Followed by Sequence Divergence and Acquisition of Distinct Functions in Trypanosoma brucei. J. Biol. Chem. 272: 10498-10505 [Abstract] [Full Text]  
• Matthews, K., Gull, K (1997). Commitment to differentiation and cell cycle re-entry are coincident but separable events in the transformation of African trypanosomes from their bloodstream to their insect form. J. Cell Sci. 110: 2609-2618 [Abstract]  
• Wirtz, E, Clayton, C (1995). Inducible gene expression in trypanosomes mediated by a prokaryotic repressor. Science 268: 1179-1183 [Abstract]  
• Acosta-Serrano, A., Vassella, E., Liniger, M., Renggli, C. K., Brun, R., Roditi, I., Englund, P. T. (2001). The surface coat of procyclic Trypanosoma brucei: Programmed expression and proteolytic cleavage of procyclin in the tsetse fly. Proc. Natl. Acad. Sci. USA 98: 1513-1518 [Abstract] [Full Text]  
• Nagamune, K., Nozaki, T., Maeda, Y., Ohishi, K., Fukuma, T., Hara, T., Schwarz, R. T., Sutterlin, C., Brun, R., Riezman, H., Kinoshita, T. (2000). Critical roles of glycosylphosphatidylinositol for Trypanosoma brucei. Proc. Natl. Acad. Sci. USA 97: 10336-10341 [Abstract] [Full Text]