This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Boylan, M T Articles by Timberlake, W E Search for Related Content PubMed PubMed Citation Articles by Boylan, M T Articles by Timberlake, W E

Isolation and physical characterization of three essential conidiation genes from Aspergillus nidulans.

Department of Genetics, University of Georgia, Athens 30602.

ABSTRACT

We cloned and characterized three genes from Aspergillus nidulans, designated brlA, abaA, and wetA, whose activities are required to complete different stages of conidiophore development. Inactivation of these genes causes major abnormalities in conidiophore morphology and prevents expression of many unrelated, developmentally regulated genes, without affecting the expression of nonregulated genes. The three genes code for poly(A)+ RNAs that begin to accumulate at different times during conidiation. The brlA- and abaA-encoded RNAs accumulate specifically in cells of the conidiophore. The wetA-encoded RNA accumulates in mature conidia. Inactivation of the brlA gene prevents expression of the abaA and wetA genes, whereas inactivation of the abaA gene prevents expression of the wetA gene. Our results confirm genetic predictions as to the temporal and spatial patterns of expression of these genes and demonstrate that these patterns are specified at the level of RNA accumulation.

This article has been cited by other articles:

• Wu, J., Ridgway, H. J., Carpenter, M. A., Glare, T. R. (2008). Identification of novel genes associated with conidiation in Beauveria bassiana with suppression subtractive hybridization.. Mycologia 100: 20-30 [Abstract] [Full Text]  
• Coyle, C. M., Kenaley, S. C., Rittenour, W. R., Panaccione, D. G. (2007). Association of ergot alkaloids with conidiation in Aspergillus fumigatus.. Mycologia 99: 804-811 [Abstract] [Full Text]  
• Breakspear, A., Momany, M. (2007). Aspergillus nidulans Conidiation Genes dewA, fluG, and stuA Are Differentially Regulated in Early Vegetative Growth. Eukaryot Cell 6: 1697-1700 [Abstract] [Full Text]  
• Yoshinari, T., Akiyama, T., Nakamura, K., Kondo, T., Takahashi, Y., Muraoka, Y., Nonomura, Y., Nagasawa, H., Sakuda, S. (2007). Dioctatin A is a strong inhibitor of aflatoxin production by Aspergillus parasiticus. Microbiology 153: 2774-2780 [Abstract] [Full Text]  
• Yu, J.-H., Mah, J.-H., Seo, J.-A. (2006). Growth and developmental control in the model and pathogenic aspergilli.. Eukaryot Cell 5: 1577-1584 [Full Text]  
• Ohara, T., Tsuge, T. (2004). FoSTUA, Encoding a Basic Helix-Loop-Helix Protein, Differentially Regulates Development of Three Kinds of Asexual Spores, Macroconidia, Microconidia, and Chlamydospores, in the Fungal Plant Pathogen Fusarium oxysporum. Eukaryot Cell 3: 1412-1422 [Abstract] [Full Text]  
• Bailey-Shrode, L., Ebbole, D. J. (2004). The fluffy Gene of Neurospora crassa Is Necessary and Sufficient to Induce Conidiophore Development. Genetics 166: 1741-1749 [Abstract] [Full Text]  
• Ohara, T., Inoue, I., Namiki, F., Kunoh, H., Tsuge, T. (2004). REN1 Is Required for Development of Microconidia and Macroconidia, but Not of Chlamydospores, in the Plant Pathogenic Fungus Fusarium oxysporum. Genetics 166: 113-124 [Abstract] [Full Text]  
• Calvo, A. M., Wilson, R. A., Bok, J. W., Keller, N. P. (2002). Relationship between Secondary Metabolism and Fungal Development. Microbiol. Mol. Biol. Rev. 66: 447-459 [Abstract] [Full Text]  
• Zuber, S., Hynes, M. J., Andrianopoulos, A. (2002). G-Protein Signaling Mediates Asexual Development at 25{degrees}C but Has No Effect on Yeast-Like Growth at 37{degrees}C in the Dimorphic Fungus Penicillium marneffei. Eukaryot Cell 1: 440-447 [Abstract] [Full Text]  
• Kellner, E. M., Adams, T. H. (2002). Mutations in sfdA and sfdB Suppress Multiple Developmental Mutations in Aspergillus nidulans. Genetics 160: 159-168 [Abstract] [Full Text]  
• Schier, N., Liese, R., Fischer, R. (2001). A Pcl-Like Cyclin of Aspergillus nidulans Is Transcriptionally Activated by Developmental Regulators and Is Involved in Sporulation. Mol. Cell. Biol. 21: 4075-4088 [Abstract] [Full Text]  
• Borneman, A. R., Hynes, M. J., Andrianopoulos, A. (2001). An STE12 Homolog From the Asexual, Dimorphic Fungus Penicillium marneffei Complements the Defect in Sexual Development of an Aspergillus nidulans steA Mutant. Genetics 157: 1003-1014 [Abstract] [Full Text]  
• Lengeler, K. B., Davidson, R. C., D'souza, C., Harashima, T., Shen, W.-C., Wang, P., Pan, X., Waugh, M., Heitman, J. (2000). Signal Transduction Cascades Regulating Fungal Development and Virulence. Microbiol. Mol. Biol. Rev. 64: 746-785 [Abstract] [Full Text]  
• Navarro, R. E., Aguirre, J. (1998). Posttranscriptional Control Mediates Cell Type-Specific Localization of Catalase A during Aspergillus nidulans Development. J. Bacteriol. 180: 5733-5738 [Abstract] [Full Text]  
• Yager, L. N., Lee, H.-O., Nagle, D. L., Zimmerman, J. E. (1998). Analysis of fluG Mutations That Affect Light-Dependent Conidiation in Aspergillus nidulans. Genetics 149: 1777-1786 [Abstract] [Full Text]  
• Adams, T. H., Wieser, J. K., Yu, J.-H. (1998). Asexual Sporulation in Aspergillus nidulans. Microbiol. Mol. Biol. Rev. 62: 35-54 [Abstract] [Full Text]  
• Wieser, J, Adams, T H (1995). flbD encodes a Myb-like DNA-binding protein that coordinates initiation of Aspergillus nidulans conidiophore development.. Genes Dev. 9: 491-502 [Abstract]  
• Lee, B N, Adams, T H (1994). The Aspergillus nidulans fluG gene is required for production of an extracellular developmental signal and is related to prokaryotic glutamine synthetase I.. Genes Dev. 8: 641-651 [Abstract]  
• Miller, K Y, Wu, J, Miller, B L (1992). StuA is required for cell pattern formation in Aspergillus.. Genes Dev. 6: 1770-1782 [Abstract]  
• Stringer, M A, Dean, R A, Sewall, T C, Timberlake, W E (1991). Rodletless, a new Aspergillus developmental mutant induced by directed gene inactivation.. Genes Dev. 5: 1161-1171 [Abstract]  
• Mooney, J L, Yager, L N (1990). Light is required for conidiation in Aspergillus nidulans.. Genes Dev. 4: 1473-1482 [Abstract]  
• Timberlake, W., Marshall, M. (1989). Genetic engineering of filamentous fungi. Science 244: 1313-1317 [Abstract]