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Mol Cell Biol. 1987 September; 7(9): 3124-3130

Characterization of an ATP-dependent DNA strand transferase from human cells.

D Ganea, P Moore, L Chekuri and R Kucherlapati

Center for Genetics, University of Illinois College of Medicine, Chicago 60612.

ABSTRACT

We have characterized an enzymatic activity from human cell nuclei which is capable of catalyzing strand exchange between homologous DNA sequences. The strand exchange activity was Mg2+ dependent and required ATP hydrolysis. In addition, it was capable of promoting reannealing of homologous DNA sequences and could form nucleoprotein networks in a fashion reminiscent of purified bacterial RecA protein. Using an in vitro recombination assay, we also showed that the strand exchange activity was biologically important. The factor(s) responsible for the activity has been partially purified.

Mol Cell Biol. 1987 September; 7(9): 3124-3130




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