This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schechtman, M G Search for Related Content PubMed PubMed Citation Articles by Schechtman, M G

Isolation of telomere DNA from Neurospora crassa.

Department of Biology, Syracuse University, New York 13244.

ABSTRACT

The most distal known gene on Neurospora crassa linkage group VR, his-6, was cloned. A genomic walk resulted in isolation of the telomere at VR. It was obtained from a library in which the endmost nucleotides of the chromosome had not been removed by nuclease treatment before being cloned, and mapping indicates that the entire chromosome end has probably been cloned. Sequences homologous to the terminal 2.5 kilobases of DNA from VR from these Oak Ridge N. crassa strains are found at other sites in the genome. To characterize these sites, I crossed an Oak Ridge-derived his-6 strain with a wild-type strain of different genetic background (Mauriceville) and characterized the hybridization patterns seen in the progeny. It appears that the sequences homologous to the VR terminus are found at genetically different sites in the two parental strains, and no hybridization to the VR telomere from Mauriceville was detected. The other genomic copies identified in the Oak Ridge parent were not telomeres. I suggest that any repeating sequence blocks found immediately adjacent to the VR terminus in Oak Ridge strains must be small and that the repeating element identified in that background may be an N. crassa transposable element integrated near the the chromosome end at VR.

This article has been cited by other articles:

• Kelkar, H. S., Griffith, J., Case, M. E., Covert, S. F., Hall, R. D., Keith, C. H., Oliver, J. S., Orbach, M. J., Sachs, M. S., Wagner, J. R., Weise, M. J., Wunderlich, J. K., Arnold, J. (2001). The Neurospora crassa Genome: Cosmid Libraries Sorted by Chromosome. Genetics 157: 979-990 [Abstract] [Full Text]  
• Cambareri, E. B., Aisner, R., Carbon, J. (1998). Structure of the Chromosome VII Centromere Region in Neurospora crassa: Degenerate Transposons and Simple Repeats. Mol. Cell. Biol. 18: 5465-5477 [Abstract] [Full Text]  
• Liu, Z P, Tye, B K (1991). A yeast protein that binds to vertebrate telomeres and conserved yeast telomeric junctions.. Genes Dev. 5: 49-59 [Abstract]