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Mol Cell Biol. 1988 January; 8(1): 267-272

Identification of the human beta-actin enhancer and its binding factor.

T Kawamoto, K Makino, H Niwa, H Sugiyama, S Kimura, M Amemura, A Nakata and T Kakunaga

Department of Oncogene Research, Osaka University, Japan.

ABSTRACT

An enhancer of the human beta-actin gene and a factor that specifically interacts with it were detected. A mobility shift assay showed that the factor bound to the 25-base-pair sequence (between +759 and +783 downstream from the cap site) with high specificity. This finding correlated with those of DNase I protection and exonuclease III digestion assays. This binding region of the beta-actin enhancer contained a hyphenated dyad symmetry and an enhancer core-like sequence. In vitro competition experiments indicated that the factor did not bind to the simian virus 40 enhancer core region.


Mol Cell Biol. 1988 January; 8(1): 267-272




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