This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rudnicki, M A Articles by McBurney, M W Search for Related Content PubMed PubMed Citation Articles by Rudnicki, M A Articles by McBurney, M W

 Previous Article  |  Next Article 

Mol Cell Biol. 1988 January; 8(1): 406-417

Regulated expression of a transfected human cardiac actin gene during differentiation of multipotential murine embryonal carcinoma cells.

M A Rudnicki, M Ruben and M W McBurney

Department of Medicine, University of Ottawa, Ontario, Canada.

ABSTRACT

P19 embryonal carcinoma (EC) cells are multipotential stem cells which can be induced to differentiate in vitro into a variety of cell types, including cardiac muscle cells. A cloned human cardiac actin (CH-actin) gene was transfected into P19 cells, and stable transformants were isolated. Low levels of CH-actin mRNA were present in transformed EC cells, but a marked increase in the level of CH-actin mRNA was found as these cells differentiated into cardiac muscle. The accumulation of CH-actin mRNA paralleled that of the endogenous mouse cardiac actin mRNA. A chimeric gene, which consisted of the CH-actin promoter linked to the herpes simplex virus thymidine kinase coding region, was constructed and transfected into P19 cells. In these transformants, the thymidine kinase protein was located almost exclusively in cardiac muscle cells and was generally not detectable in EC or other nonmuscle cells. These results suggest that the transfected CH-actin promoter functions in the appropriate developmental and tissue-specific manner during the differentiation of multipotential EC cells in culture.

---

Mol Cell Biol. 1988 January; 8(1): 406-417

This article has been cited by other articles:

• van der Heyden, M. A.G., Defize, L. H.K. (2003). Twenty one years of P19 cells: what an embryonal carcinoma cell line taught us about cardiomyocyte differentiation. Cardiovasc Res 58: 292-302 [Abstract] [Full Text]  
• Zhang, A., Potvin, B., Zaiman, A., Chen, W., Kumar, R., Phillips, L., Stanley, P. (1999). The Gain-of-Function Chinese Hamster Ovary Mutant LEC11B Expresses One of Two Chinese Hamster FUT6 Genes Due to the Loss of a Negative Regulatory Factor. J. Biol. Chem. 274: 10439-10450 [Abstract] [Full Text]  
• Suzuki, T., Kim, H.-S., Kurabayashi, M., Hamada, H., Fujii, H., Aikawa, M., Watanabe, M., Watanabe, N., Sakomura, Y., Yazaki, Y., Nagai, R. (1996). Preferential Differentiation of P19 Mouse Embryonal Carcinoma Cells Into Smooth Muscle Cells : Use of Retinoic Acid and Antisense Against the Central Nervous System–Specific POU Transcription Factor Brn-2. Circ. Res. 78: 395-404 [Abstract] [Full Text]  
• Vidricaire, G, Jardine, K, McBurney, M. (1994). Expression of the Brachyury gene during mesoderm development in differentiating embryonal carcinoma cell cultures. Development 120: 115-122 [Abstract]