This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Prowse, K R Articles by Baumann, H Search for Related Content PubMed PubMed Citation Articles by Prowse, K R Articles by Baumann, H

Hepatocyte-stimulating factor, beta 2 interferon, and interleukin-1 enhance expression of the rat alpha 1-acid glycoprotein gene via a distal upstream regulatory region.

Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, Buffalo, New York 14263.

ABSTRACT

The rat alpha 1-acid glycoprotein (AGP) gene is transcriptionally regulated by dexamethasone, interleukin 1 (IL-1), hepatocyte-stimulating factor, and beta 2 interferon. The steroid and peptide hormones stimulate expression of the AGP gene synergistically as well as independently. The regulatory sequence responsible for dexamethasone-stimulated expression has been localized previously to a region that is 120 to 64 base pairs (bp) upstream of the transcription start site (H. Baumann and L. E. Maquat, Mol. Cell. Biol. 6:2551-2561, 1986). To identify the regulatory sequence that is responsive to the peptide hormones, different lengths of the AGP gene 5'-flanking DNA were linked to the chloramphenicol acetyltransferase gene and then assayed for hormone-inducible chloramphenicol acetyltransferase gene expression in transiently transfected HepG2 cells. We demonstrate that an enhancer region that is responsive to IL-1, hepatocyte-stimulating factor, and beta 2 interferon lies within a 142-bp sequence located 5,300 to 5,150 bp upstream of the transcription start site. This distal regulatory region can confer hormone inducibility to a heterologous promoter; exert its affect in either orientation; and function, to a lesser degree, in nonhepatic but IL-1-responsive cells.

This article has been cited by other articles:

• Isaksen, D. E., Baumann, H., Trobridge, P. A., Farr, A. G., Levin, S. D., Ziegler, S. F. (1999). Requirement for Stat5 in Thymic Stromal Lymphopoietin-Mediated Signal Transduction. J. Immunol. 163: 5971-5977 [Abstract] [Full Text]  
• Kim, H., Hawley, T. S., Hawley, R. G., Baumann, H. (1998). Protein Tyrosine Phosphatase 2 (SHP-2) Moderates Signaling by gp130 but Is Not Required for the Induction of Acute-Phase Plasma Protein Genes in Hepatic Cells. Mol. Cell. Biol. 18: 1525-1533 [Abstract] [Full Text]  
• Kuropatwinski, K. K., De Imus, C., Gearing, D., Baumann, H., Mosley, B. (1997). Influence of Subunit Combinations on Signaling by Receptors for Oncostatin M, Leukemia Inhibitory Factor, and Interleukin-6. J. Biol. Chem. 272: 15135-15144 [Abstract] [Full Text]  
• Kim, H., Baumann, H. (1997). The Carboxyl-terminal Region of STAT3 Controls Gene Induction by the Mouse Haptoglobin Promoter. J. Biol. Chem. 272: 14571-14579 [Abstract] [Full Text]  
• Kordula, T., Ripperger, J., Morella, K. K., Travis, J., Baumann, H. (1996). Two Separate Signal Transducer and Activator of Transcription Proteins Regulate Transcription of the Serine Proteinase Inhibitor-3 Gene in Hepatic Cells. J. Biol. Chem. 271: 6752-6757 [Abstract] [Full Text]  
• Morella, K. K., Lai, C.-f., Kumaki, S., Kumaki, N., Wang, Y., Bluman, E. M., Witthuhn, B. A., Ihle, J. N., Giri, J., Gearing, D. P., Cosman, D., Ziegler, S. F., Tweardy, D. J., Campos, S. P., Baumann, H. (1995). The Action of Interleukin-2 Receptor Subunits Defines a New Type of Signaling Mechanism for Hematopoietin Receptors in Hepatic Cells and Fibroblasts. J. Biol. Chem. 270: 8298-8310 [Abstract] [Full Text]