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Mol Cell Biol. 1988 November; 8(11): 4936-4948

Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases.

J S Robinson, D J Klionsky, L M Banta and S D Emr

Division of Biology, California Institute of Technology, Pasadena 91125.

ABSTRACT

Using a selection for spontaneous mutants that mislocalize a vacuolar carboxypeptidase Y (CPY)-invertase fusion protein to the cell surface, we identified vacuolar protein targeting (vpt) mutants in 25 new vpt complementation groups. Additional alleles in each of the eight previously identified vpt complementation groups (vpt1 through vpt8) were also obtained. Representative alleles from each of the 33 vpt complementation groups (vpt1 through vpt33) were shown to exhibit defects in the sorting and processing of several native vacuolar proteins, including the soluble hydrolases CPY, proteinase A, and proteinase B. Of the 33 complementation groups, 19 were found to contain mutant alleles that led to extreme defects. In these mutants, CPY accumulated in its Golgi complex-modified precursor form which was secreted by the mutant cells. Normal protein secretion appeared to be unaffected in the vpt mutants. The lack of significant leakage of cytosolic markers from the vpt mutant cells indicated that the vacuolar protein-sorting defects associated with these mutants do not result from cell lysis. In addition, the observation that the precursor rather than the mature forms of CPY, proteinase A, proteinase B were secreted from the vpt mutants was consistent with the fact that mislocalization occurred at a stage after Golgi complex-specific modification, but before final vacuolar sorting of these enzymes. Vacuolar membrane protein sorting appeared to be unaffected in the majority of the vpt mutants. However, a subset of the vpt mutants (vpt11, vpt16, vpt18, and vpt33) was found to exhibit defects in the sorting of a vacuolar membrane marker enzyme, alpha-mannosidase. Up to 50% of the alpha-mannosidase enzyme activity was found to be mislocalized to the cell surface in these vpt mutants. Seven of the vpt complementation groups (vpt3, vpt11, vpt15, vpt16, vpt18, vpt29, and vpt33) contained alleles that led to a conditional lethal phenotype; the mutants were temperature sensitive for vegetative cell growth. This temperature-sensitive phenotype has been shown to be recessive and to cosegregate with the vacuolar protein-sorting defect in each case. Tetrad analysis showed that vpt3 mapped to the right arm of chromosome XV and that vpt15 mapped to the right arm of chromosome II. Intercrosses with other mutants that exhibited defects in vacuolar protein sorting or function (vpl, sec, pep, and end mutants) revealed several overlaps among these different sets of genes. Together, these data indicate that more than 50 gene products are involved, directly or indirectly, in the process of vacuolar protein sorting.

Mol Cell Biol. 1988 November; 8(11): 4936-4948




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  • Hua, Z., Graham, T. R. (2003). Requirement for Neo1p in Retrograde Transport from the Golgi Complex to the Endoplasmic Reticulum. Mol. Biol. Cell 14: 4971-4983 [Abstract] [Full Text]  
  • Tucker, K. A., Reggiori, F., Dunn, W. A. Jr., Klionsky, D. J. (2003). Atg23 Is Essential for the Cytoplasm to Vacuole Targeting Pathway and Efficient Autophagy but Not Pexophagy. J. Biol. Chem. 278: 48445-48452 [Abstract] [Full Text]  
  • Uemura, S., Kihara, A., Inokuchi, J.-i., Igarashi, Y. (2003). Csg1p and Newly Identified Csh1p Function in Mannosylinositol Phosphorylceramide Synthesis by Interacting with Csg2p. J. Biol. Chem. 278: 45049-45055 [Abstract] [Full Text]  
  • Malinska, K., Malinsky, J., Opekarova, M., Tanner, W. (2003). Visualization of Protein Compartmentation within the Plasma Membrane of Living Yeast Cells. Mol. Biol. Cell 14: 4427-4436 [Abstract] [Full Text]  
  • Bieganowski, P., Pace, H. C., Brenner, C. (2003). Eukaryotic NAD+ Synthetase Qns1 Contains an Essential, Obligate Intramolecular Thiol Glutamine Amidotransferase Domain Related to Nitrilase. J. Biol. Chem. 278: 33049-33055 [Abstract] [Full Text]  
  • Yoko-o, T., Wiggins, C. A.R., Stolz, J., Peak-Chew, S. Y., Munro, S. (2003). An N-acetylglucosaminyltransferase of the Golgi apparatus of the yeast Saccharomyces cerevisiae that can modify N-linked glycans. Glycobiology 13: 581-589 [Abstract] [Full Text]  
  • Palmer, G. E., Cashmore, A., Sturtevant, J. (2003). Candida albicans VPS11 Is Required for Vacuole Biogenesis and Germ Tube Formation. Eukaryot Cell 2: 411-421 [Abstract] [Full Text]  
  • Davies, B. A., Topp, J. D., Sfeir, A. J., Katzmann, D. J., Carney, D. S., Tall, G. G., Friedberg, A. S., Deng, L., Chen, Z., Horazdovsky, B. F. (2003). Vps9p CUE Domain Ubiquitin Binding Is Required for Efficient Endocytic Protein Traffic. J. Biol. Chem. 278: 19826-19833 [Abstract] [Full Text]  
  • Odorizzi, G., Katzmann, D. J., Babst, M., Audhya, A., Emr, S. D. (2003). Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae. J. Cell Sci. 116: 1893-1903 [Abstract] [Full Text]  
  • Sriram, V., Krishnan, K.S., Mayor, S. (2003). deep-orange and carnation define distinct stages in late endosomal biogenesis in Drosophila melanogaster. JCB 161: 593-607 [Abstract] [Full Text]  


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