This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dijkwel, P A
Right arrow Articles by Hamlin, J L
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dijkwel, P A
Right arrow Articles by Hamlin, J L

 Previous Article  |  Next Article 

Mol Cell Biol. 1988 December; 8(12): 5398-5409

Matrix attachment regions are positioned near replication initiation sites, genes, and an interamplicon junction in the amplified dihydrofolate reductase domain of Chinese hamster ovary cells.

P A Dijkwel and J L Hamlin

Department of Biochemistry and Cell, University of Virginia School of Medicine, Charlottesville 22908.

ABSTRACT

Genomic DNA in higher eucaryotic cells is organized into a series of loops, each of which may be affixed at its base to the nuclear matrix via a specific matrix attachment region (MAR). In this report, we describe the distribution of MARs within the amplified dihydrofolate reductase (DHFR) domain (amplicon) in the methotrexate-resistant CHO cell line CHOC 400. In one experimental protocol, matrix-attached and loop DNA fractions were prepared from matrix-halo structures by restriction digestion and were analyzed for the distribution of amplicon sequences between the two fractions. A second, in vitro method involved the specific binding to the matrix of cloned DNA fragments from the amplicon. Both methods of analysis detected a MAR in the replication initiation locus that we have previously defined in the DHFR amplicon, as well as in the 5'-flanking region of the DHFR gene. The first of these methods also suggests the presence of a MAR in a region mapping approximately 120 kilobases upstream from the DHFR gene. Each of these MARs was detected regardless of whether the matrix-halo structures were prepared by the high-salt or the lithium 3,5-diiodosalicylate extraction protocols, arguing against their artifactual association with the proteinaceous scaffolding of the nucleus during isolation procedures. However, the in vitro binding assay did not detect the MAR located 120 kilobases upstream from the DHFR gene but did detect specific matrix attachment of a sequence near the junction between amplicons. The results of these experiments suggest that (i) MARs can occur next to different functional elements in the genome, with the result that a DNA loop formed between two MARs can be smaller than a replicon; and (ii) different methods of analysis detect a somewhat different spectrum of matrix-attached DNA fragments.

Mol Cell Biol. 1988 December; 8(12): 5398-5409




This article has been cited by other articles:

  • Wang, C.-Y., Sugden, B. (2008). Identifying a property of origins of DNA synthesis required to support plasmids stably in human cells. Proc. Natl. Acad. Sci. USA 105: 9639-9644 [Abstract] [Full Text]  
  • Lufino, M. M.P., Manservigi, R., Wade-Martins, R. (2007). An S/MAR-based infectious episomal genomic DNA expression vector provides long-term regulated functional complementation of LDLR deficiency. Nucleic Acids Res 0: gkm570v1-10 [Abstract] [Full Text]  
  • Miccoli, L., Frouin, I., Novac, O., Di Paola, D., Harper, F., Zannis-Hadjopoulos, M., Maga, G., Biard, D. S. F., Angulo, J. F. (2005). The Human Stress-Activated Protein kin17 Belongs to the Multiprotein DNA Replication Complex and Associates In Vivo with Mammalian Replication Origins. Mol. Cell. Biol. 25: 3814-3830 [Abstract] [Full Text]  
  • Naryzhny, S. N., Lee, H. (2004). The Post-translational Modifications of Proliferating Cell Nuclear Antigen: ACETYLATION, NOT PHOSPHORYLATION, PLAYS AN IMPORTANT ROLE IN THE REGULATION OF ITS FUNCTION. J. Biol. Chem. 279: 20194-20199 [Abstract] [Full Text]  
  • Feng, Z., Hu, W., Chasin, L. A., Tang, M.-s. (2003). Effects of genomic context and chromatin structure on transcription-coupled and global genomic repair in mammalian cells. Nucleic Acids Res 31: 5897-5906 [Abstract] [Full Text]  
  • Shimizu, N., Hashizume, T., Shingaki, K., Kawamoto, J.-k. (2003). Amplification of Plasmids Containing a Mammalian Replication Initiation Region Is Mediated by Controllable Conflict between Replication and Transcription. Cancer Res. 63: 5281-5290 [Abstract] [Full Text]  
  • Miccoli, L., Biard, D. S. F., Frouin, I., Harper, F., Maga, G., Angulo, J. F. (2003). Selective interactions of human kin17 and RPA proteins with chromatin and the nuclear matrix in a DNA damage- and cell cycle-regulated manner. Nucleic Acids Res 31: 4162-4175 [Abstract] [Full Text]  
  • Mollenbeck, M., Postberg, J., Paeschke, K., Rossbach, M., Jonsson, F., Lipps, H. J. (2003). The telomerase-associated protein p43 is involved in anchoring telomerase in the nucleus. J. Cell Sci. 116: 1757-1761 [Abstract] [Full Text]  
  • Mesner, L. D., Hamlin, J. L., Dijkwel, P. A. (2003). The matrix attachment region in the Chinese hamster dihydrofolate reductase origin of replication may be required for local chromatid separation. Proc. Natl. Acad. Sci. USA 100: 3281-3286 [Abstract] [Full Text]  
  • Mesner, L. D., Li, X., Dijkwel, P. A., Hamlin, J. L. (2003). The Dihydrofolate Reductase Origin of Replication Does Not Contain Any Nonredundant Genetic Elements Required for Origin Activity. Mol. Cell. Biol. 23: 804-814 [Abstract] [Full Text]  
  • Fujita, M., Ishimi, Y., Nakamura, H., Kiyono, T., Tsurumi, T. (2002). Nuclear Organization of DNA Replication Initiation Proteins in Mammalian Cells. J. Biol. Chem. 277: 10354-10361 [Abstract] [Full Text]  
  • Shimizu, N., Miura, Y., Sakamoto, Y., Tsutsui, K. (2001). Plasmids with a Mammalian Replication Origin and a Matrix Attachment Region Initiate the Event Similar to Gene Amplification. Cancer Res. 61: 6987-6990 [Abstract] [Full Text]  
  • Smilenov, L. B., Dhar, S., Pandita, T. K. (1999). Altered Telomere Nuclear Matrix Interactions and Nucleosomal Periodicity in Ataxia Telangiectasia Cells before and after Ionizing Radiation Treatment. Mol. Cell. Biol. 19: 6963-6971 [Abstract] [Full Text]  
  • Pandita, T. K., Westphal, C. H., Anger, M., Sawant, S. G., Geard, C. R., Pandita, R. K., Scherthan, H. (1999). Atm Inactivation Results in Aberrant Telomere Clustering during Meiotic Prophase. Mol. Cell. Biol. 19: 5096-5105 [Abstract] [Full Text]  
  • Ortega, J., DePamphilis, M. (1999). Nucleoskeleton and initiation of DNA replication in metazoan cells. J. Cell Sci. 111: 3663-3673 [Abstract]  
  • Sakai, E., Bottaro, A., Davidson, L., Sleckman, B. P., Alt, F. W. (1999). Recombination and transcription of the endogenous Ig heavy chain locus is effected by the Ig heavy chain intronic enhancer core region in the absence of the matrix attachment regions. Proc. Natl. Acad. Sci. USA 96: 1526-1531 [Abstract] [Full Text]  
  • Pemov, A., Bavykin, S., Hamlin, J. L. (1998). Attachment to the nuclear matrix mediates specific alterations in chromatin structure. Proc. Natl. Acad. Sci. USA 95: 14757-14762 [Abstract] [Full Text]  
  • de Belle, I., Cai, S., Kohwi-Shigematsu, T. (1998). The Genomic Sequences Bound to Special AT-rich Sequence-binding Protein 1 (SATB1) In Vivo in Jurkat T Cells Are Tightly Associated with the Nuclear Matrix at the Bases of the Chromatin Loops. JCB 141: 335-348 [Abstract] [Full Text]  
  • Wang, S., Dijkwel, P. A., Hamlin, J. L. (1998). Lagging-Strand, Early-Labelling, and Two-Dimensional Gel Assays Suggest Multiple Potential Initiation Sites in the Chinese Hamster Dihydrofolate Reductase Origin. Mol. Cell. Biol. 18: 39-50 [Abstract] [Full Text]  
  • Lee, H., Larner, J. M., Hamlin, J. L. (1997). A p53-independent damage-sensing mechanism that functions as a checkpoint at the G1/S transition in Chinese hamster ovary cells. Proc. Natl. Acad. Sci. USA 94: 526-531 [Abstract] [Full Text]  
  • Kramer, J. A., Krawetz, S. A. (1996). Nuclear Matrix Interactions within the Sperm Genome. J. Biol. Chem. 271: 11619-11622 [Abstract] [Full Text]  
  • Mah, D., Dijkwel, P., Todd, A, Klein, V, Price, G., Zannis-Hadjopoulos, M (1993). ors12, a mammalian autonomously replicating DNA sequence, associates with the nuclear matrix in a cell cycle-dependent manner. J. Cell Sci. 105: 807-818 [Abstract]  
  • Hamlin, J.L., Mosca, P.J., Dijkwel, P.A., Lin, H.-B. (1993). Initiation of Replication at a Mammalian Chromosomal Origin. Cold Spring Harb Symp Quant Biol 58: 467-474 [Abstract]  
  • Bode, J, Kohwi, Y, Dickinson, L, Joh, T, Klehr, D, Mielke, C, Kohwi-Shigematsu, T (1992). Biological significance of unwinding capability of nuclear matrix-associating DNAs. Science 255: 195-197 [Abstract]  
  • Schaack, J, Ho, W Y, Freimuth, P, Shenk, T (1990). Adenovirus terminal protein mediates both nuclear matrix association and efficient transcription of adenovirus DNA.. Genes Dev. 4: 1197-1208 [Abstract]  
  • Trask, B J, Hamlin, J L (1989). Early dihydrofolate reductase gene amplification events in CHO cells usually occur on the same chromosome arm as the original locus.. Genes Dev. 3: 1913-1925 [Abstract]  
  • Alexandrow, M. G., Ritzi, M., Pemov, A., Hamlin, J. L. (2002). A Potential Role for Mini-chromosome Maintenance (MCM) Proteins in Initiation at the Dihydrofolate Reductase Replication Origin. J. Biol. Chem. 277: 2702-2708 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»