This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Furdon, P J Articles by Kole, R Search for Related Content PubMed PubMed Citation Articles by Furdon, P J Articles by Kole, R

The length of the downstream exon and the substitution of specific sequences affect pre-mRNA splicing in vitro.

Lineberger Cancer Research Center, University of North Carolina, Chapel Hill 27514.

ABSTRACT

We have shown previously that truncation of the human beta-globin pre-mRNA in the second exon, 14 nucleotides downstream from the 3' splice site, leads to inhibition of splicing but not cleavage at the 5' splice site. We now show that several nonglobin sequences substituted at this site can restore splicing and that the efficiency of splicing depends on the length of the second (downstream) exon and not a specific sequence. Deletions in the first exon have no effect on the efficiency of in vitro splicing. Surprisingly, an intron fragment from the 5' region of the human or rabbit beta-globin intron 2, when placed 14 nucleotides downstream from the 3' splice site, inhibited all the steps in splicing beginning with cleavage at the 5' splice site. This result suggests that the intron 2 fragment carries a "poison" sequence that can inhibit the splicing of an upstream intron.

This article has been cited by other articles:

• Fairbrother, W. G., Chasin, L. A. (2000). Human Genomic Sequences That Inhibit Splicing. Mol. Cell. Biol. 20: 6816-6825 [Abstract] [Full Text]  
• De Backer, O., Arden, K. C., Boretti, M., Vantomme, V., De Smet, C., Czekay, S., Viars, C. S., De Plaen, E., Brasseur, F., Chomez, P., Van den Eynde, B., Boon, T., van der Bruggen, P. (1999). Characterization of the GAGE Genes That Are Expressed in Various Human Cancers and in Normal Testis. Cancer Res. 59: 3157-3165 [Abstract] [Full Text]  
• Zheng, Z. M., He, P.-j., Baker, C. C. (1999). Function of a Bovine Papillomavirus Type 1 Exonic Splicing Suppressor Requires a Suboptimal Upstream 3' Splice Site. J. Virol. 73: 29-36 [Abstract] [Full Text]  
• Schaal, T. D., Maniatis, T. (1999). Multiple Distinct Splicing Enhancers in the Protein-Coding Sequences of a Constitutively Spliced Pre-mRNA. Mol. Cell. Biol. 19: 261-273 [Abstract] [Full Text]  
• Kosaki, A., Nelson, J., Webster, N. J. G. (1998). Identification of Intron and Exon Sequences Involved in Alternative Splicing of Insulin Receptor Pre-mRNA. J. Biol. Chem. 273: 10331-10337 [Abstract] [Full Text]  
• Kohrman, D. C., Harris, J. B., Meisler, M. H. (1996). Mutation Detection in the med and medJ Alleles of the Sodium Channel Scn8a. UNUSUAL SPLICING DUE TO A MINOR CLASS AT-AC INTRON. J. Biol. Chem. 271: 17576-17581 [Abstract] [Full Text]  
• Watakabe, A, Tanaka, K, Shimura, Y (1993). The role of exon sequences in splice site selection.. Genes Dev. 7: 407-418 [Abstract]  
• Goguel, V, Liao, X L, Rymond, B C, Rosbash, M (1991). U1 snRNP can influence 3'-splice site selection as well as 5'-splice site selection.. Genes Dev. 5: 1430-1438 [Abstract]  
• Helfman, D M, Ricci, W M, Finn, L A (1988). Alternative splicing of tropomyosin pre-mRNAs in vitro and in vivo.. Genes Dev. 2: 1627-1638 [Abstract]  
• Kedes, D H, Steitz, J A (1988). Correct in vivo splicing of the mouse immunoglobulin kappa light-chain pre-mRNA is dependent on 5' splice-site position even in the absence of transcription.. Genes Dev. 2: 1448-1459 [Abstract]