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Mol Cell Biol. 1988 June; 8(6): 2494-2503

Differential protein phosphorylation in induction of thyroid cell proliferation by thyrotropin, epidermal growth factor, or phorbol ester.

Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, Belgium.

ABSTRACT

Protein phosphorylation was studied in primary cultures of thyroid epithelial cells after the addition of different mitogens: thyrotropin (TSH) acting through cyclic AMP, epidermal growth factor (EGF), or 12-O-tetradecanoylphorbol-13-acetate (TPA). EGF or TPA increased the phosphorylation of five common polypeptides. Among these, two 42-kilodalton proteins contained phosphotyrosine and phosphoserine with or without phosphothreonine. Their characteristics suggested that they are similar to the two 42-kilodalton target proteins for tyrosine protein phosphorylation demonstrated in fibroblasts in response to mitogens. No common phosphorylated proteins were detected in TSH-treated cells and in EGF- or TPA-treated cells. The differences in the protein phosphorylation patterns in response to TSH, EGF, and TPA suggested that the newly emerging cyclic AMP-mediated mitogenic pathway is distinct from the better known growth factor- and tumor promoter-induced pathways.

This article has been cited by other articles:

• Burikhanov, R., Coulonval, K., Pirson, I., Lamy, F., Dumont, J. E., Roger, P. P. (1996). Thyrotropin via Cyclic AMP Induces Insulin Receptor Expression and Insulin Co-stimulation of Growth and Amplifies Insulin and Insulin-like Growth Factor Signaling Pathways in Dog Thyroid Epithelial Cells. J. Biol. Chem. 271: 29400-29406 [Abstract] [Full Text]  
• Baptist, M, Dumont, J., Roger, P. (1993). Demonstration of cell cycle kinetics in thyroid primary culture by immunostaining of proliferating cell nuclear antigen: differences in cyclic AMP-dependent and -independent mitogenic stimulations. J. Cell Sci. 105: 69-80 [Abstract]