This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Camonis, J H Articles by Jacquet, M Search for Related Content PubMed PubMed Citation Articles by Camonis, J H Articles by Jacquet, M

A new RAS mutation that suppresses the CDC25 gene requirement for growth of Saccharomyces cerevisiae.

Groupe Information Génétique et Développement, Université de Paris-Sud, Orsay, France.

ABSTRACT

In the yeast Saccharomyces cerevisiae, the activation of adenylate cyclase requires the products of the RAS genes and of CDC25. We isolated several dominant extragenic suppressors of the yeast cdc25 mutation. They did not suppress a thermosensitive allele of the adenylate cyclase gene (CDC35). One of these suppressors was a mutated RAS2 gene in which the transition C/G----T/A at position 455 resulted in replacement of threonine 152 by isoleucine in the protein. The same mutation in a v-Ha-ras gene reduces the affinity of p21 for guanine nucleotides (L.A. Feig, B. Pan, T.M. Roberts, and G.M. Cooper, Proc. Natl. Acad. Sci. USA 83:4607-4611, 1986). These results support a model in which the CDC25 gene product is the GDP-GTP exchange factor regulating the activity of the RAS gene product.

This article has been cited by other articles:

• COLOMBO, S., PERI, F., TISI, R., NICOTRA, F., MARTEGANI, E. (2004). Design and Characterization of a New Class of Inhibitors of Ras Activation. Ann. N. Y. Acad. Sci. 1030: 52-61 [Abstract] [Full Text]  
• Wang, L., Renault, G., Garreau, H., Jacquet, M. (2004). Stress induces depletion of Cdc25p and decreases the cAMP producing capability in Saccharomyces cerevisiae. Microbiology 150: 3383-3391 [Abstract] [Full Text]  
• Ogg, S. C., Barz, W. P., Walter, P. (1998). A Functional GTPase Domain, but not its Transmembrane Domain, is Required for Function of the SRP Receptor beta -subunit. J. Cell Biol. 142: 341-354 [Abstract] [Full Text]  
• Kaplon, T., Jacquet, M. (1995). The Cellular Content of Cdc25p, the Ras Exchange Factor in Saccharomyces cerevisiae, Is Regulated by Destabilization Through a Cyclin Destruction Box. J. Biol. Chem. 270: 20742-20747 [Abstract] [Full Text]  
• Chardin, P, Camonis, J., Gale, N., van Aelst, L, Schlessinger, J, Wigler, M., Bar-Sagi, D (1993). Human Sos1: a guanine nucleotide exchange factor for Ras that binds to GRB2. Science 260: 1338-1343 [Abstract]