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Mol Cell Biol. 1989 October; 9(10): 4576-4580

Evidence for glycosyl-phosphatidylinositol anchoring of Toxoplasma gondii major surface antigens.

S Tomavo, R T Schwarz and J F Dubremetz

Unite' 42, Institut National de la Recheche Médicale, Villeneuve d'Ascq, France.

ABSTRACT

The four major surface antigens of Toxoplasma gondii tachyzoites (P43, P35, P30, and P22) were made water soluble by phosphatidylinositol-specific phospholipase C (PI-PLC). These antigens were biosynthetically labeled with 3H-fatty acids, [3H]ethanolamine, and [3H]carbohydrates. Treatment of 3H-fatty-acid-labeled parasite lysates with PI-PLC removed the radioactive label from these antigens. A cross-reacting determinant was exposed on these antigens after PI-PLC treatment.

Mol Cell Biol. 1989 October; 9(10): 4576-4580




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