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Mol Cell Biol. 1989 November; 9(11): 4605-4612

Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway.

Imperial Cancer Research Fund, London, United Kingdom.

ABSTRACT

We have selected mutations in genes encoding components of the signaling pathway for alpha interferon (IFN-alpha) by using a specially constructed cell line. The upstream region of the IFN-regulated human gene 6-16 was fused to the Escherichia coli guanine phosphoribosyltransferase (gpt) gene and transfected into hypoxanthine-guanine phosphoribosyltransferase-negative human cells. These cells express gpt only in the presence of IFN-alpha. They grow in medium containing hypoxanthine, aminopterin, and thymidine plus IFN and are killed by 6-thioguanine plus IFN. Two different types of mutants were obtained after treating the cells with mutagens. A recessive mutant, selected in 6-thioguanine plus IFN, was completely resistant to IFN-alpha but responded normally to IFN-gamma and, unexpectedly, partially to IFN-beta. A constitutive mutant, selected in hypoxanthine-aminopterin-thymidine alone, was abnormal in expressing endogenous genes in the absence of IFN. Both types revert infrequently, allowing selection for complementation of the defects by transfection.

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• Rani, M. R. S., Gauzzi, C., Pellegrini, S., Fish, E. N., Wei, T., Ransohoff, R. M. (1999). Induction of beta -R1/I-TAC by Interferon-beta Requires Catalytically Active TYK2. J. Biol. Chem. 274: 1891-1897 [Abstract] [Full Text]  
• Piskurich, J. F., Linhoff, M. W., Wang, Y., Ting, J. P.-Y. (1999). Two Distinct Gamma Interferon-Inducible Promoters of the Major Histocompatibility Complex Class II Transactivator Gene Are Differentially Regulated by STAT1, Interferon Regulatory Factor 1, and Transforming Growth Factor beta. Mol. Cell. Biol. 19: 431-440 [Abstract] [Full Text]  
• Richter, M. F., Dumenil, G., Uze, G., Fellous, M., Pellegrini, S. (1998). Specific Contribution of Tyk2 JH Regions to the Binding and the Expression of the Interferon alpha /beta Receptor Component IFNAR1. J. Biol. Chem. 273: 24723-24729 [Abstract] [Full Text]  
• Delude, R. L., Yoshimura, A., Ingalls, R. R., Golenbock, D. T. (1998). Construction of a Lipopolysaccharide Reporter Cell Line and Its Use in Identifying Mutants Defective in Endotoxin, But Not TNF-{alpha}, Signal Transduction. J. Immunol. 161: 3001-3009 [Abstract] [Full Text]  
• Leaman, D. W., Salvekar, A., Patel, R., Sen, G. C., Stark, G. R. (1998). A mutant cell line defective in response to double-stranded RNA and in regulating basal expression of interferon-stimulated genes. Proc. Natl. Acad. Sci. USA 95: 9442-9447 [Abstract] [Full Text]  
• Stancato, L. F., Yu, C.-R., Petricoin III, E. F., Larner, A. C. (1998). Activation of Raf-1 by Interferon gamma  and Oncostatin M Requires Expression of the Stat1 Transcription Factor. J. Biol. Chem. 273: 18701-18704 [Abstract] [Full Text]  
• Runkel, L., Pfeffer, L., Lewerenz, M., Monneron, D., Yang, C. H., Murti, A., Pellegrini, S., Goelz, S., Uze, G., Mogensen, K. (1998). Differences in Activity between alpha  and beta  Type I Interferons Explored by Mutational Analysis. J. Biol. Chem. 273: 8003-8008 [Abstract] [Full Text]  
• Wood, S. M., Wiesener, M. S., Yeates, K. M., Okada, N., Pugh, C. W., Maxwell, P. H., Ratcliffe, P. J. (1998). Selection and Analysis of a Mutant Cell Line Defective in the Hypoxia-inducible Factor-1 alpha -Subunit (HIF-1alpha ). CHARACTERIZATION OF HIF-1alpha -DEPENDENT AND -INDEPENDENT HYPOXIA-INDUCIBLE GENE EXPRESSION. J. Biol. Chem. 273: 8360-8368 [Abstract] [Full Text]  
• Domanski, P., Nadeau, O. W., Platanias, L. C., Fish, E., Kellum, M., Pitha, P., Colamonici, O. R. (1998). Differential Use of the beta L Subunit of the Type I Interferon (IFN) Receptor Determines Signaling Specificity for IFNalpha 2 and IFNbeta. J. Biol. Chem. 273: 3144-3147 [Abstract] [Full Text]  
• Piskurich, J. F., Wang, Y., Linhoff, M. W., White, L. C., Ting, J. P.-Y. (1998). Identification of Distinct Regions of 5' Flanking DNA That Mediate Constitutive, IFN-{gamma}, STAT1, and TGF-{beta}-Regulated Expression of the Class II Transactivator Gene. J. Immunol. 160: 233-240 [Abstract] [Full Text]  
• Karpusas, M., Nolte, M., Benton, C. B., Meier, W., Lipscomb, W. N., Goelz, S. (1997). The crystal structure of human interferon beta  at 2.2-A resolution. Proc. Natl. Acad. Sci. USA 94: 11813-11818 [Abstract] [Full Text]  
• Gauzzi, M. C., Barbieri, G., Richter, M. F., Uze, G., Ling, L., Fellous, M., Pellegrini, S. (1997). The amino-terminal region of Tyk2 sustains the level of interferon alpha  receptor 1, a component of the interferon alpha /beta  receptor. Proc. Natl. Acad. Sci. USA 94: 11839-11844 [Abstract] [Full Text]  
• Khabar, K. S.A., Al-Zoghaibi, F., Al-Ahdal, M. N., Murayama, T., Dhalla, M., Mukaida, N., Taha, M., Al-Sedairy, S. T., Siddiqui, Y., Kessie, G., Matsushima, K. (1997). The alpha  Chemokine, Interleukin 8, Inhibits the Antiviral Action of Interferon alpha  . J. Exp. Med. 186: 1077-1085 [Abstract] [Full Text]  
• Burfoot, M. S., Rogers, N. C., Watling, D., Smith, J. M., Pons, S., Paonessaw, G., Pellegrini, S., White, M. F., Kerr, I. M. (1997). Janus Kinase-dependent Activation of Insulin Receptor Substrate 1 in Response to Interleukin-4, Oncostatin M, and the Interferons. J. Biol. Chem. 272: 24183-24190 [Abstract] [Full Text]  
• Wadler, S., Schwartz, E. L. (1997). New Advances in Interferon Therapy of Cancer. The Oncologist 2: 254-267 [Abstract] [Full Text]  
• Shimoda, K., Feng, J., Murakami, H., Nagata, S., Watling, D., Rogers, N. C., Stark, G. R., Kerr, I. M., Ihle, J. N. (1997). Jak1 Plays an Essential Role for Receptor Phosphorylation and Stat Activation in Response to Granulocyte Colony-Stimulating Factor. Blood 90: 597-604 [Abstract] [Full Text]  
• Bluyssen, HansA.R., Levy, DavidE. (1997). Stat2 Is a Transcriptional Activator That Requires Sequence-specific Contacts Provided by Stat1 and p48 for Stable Interaction with DNA. J. Biol. Chem. 272: 4600-4605 [Abstract] [Full Text]  
• Croze, E., Russell-Harde, D., Wagner, T. C., Pu, H., Pfeffer, L. M., Perez, H. D. (1996). The Human Type I Interferon Receptor. IDENTIFICATION OF THE INTERFERON beta -SPECIFIC RECEPTOR-ASSOCIATED PHOSPHOPROTEIN. J. Biol. Chem. 271: 33165-33168 [Abstract] [Full Text]  
• Gibbs, V. C., Takahashi, M., Aguet, M., Chuntharapai, A. (1996). A Negative Regulatory Region in the Intracellular Domain of the Human Interferon-alpha Receptor. J. Biol. Chem. 271: 28710-28716 [Abstract] [Full Text]  
• Rani, M.R. S., Foster, G. R., Leung, S., Leaman, D., Stark, G. R., Ransohoff, R. M. (1996). Characterization of beta -R1, a Gene That Is Selectively Induced by Interferon beta (IFN-beta ) Compared with IFN-alpha. J. Biol. Chem. 271: 22878-22884 [Abstract] [Full Text]  
• Gauzzi, M. C., Velazquez, L., McKendry, R., Mogensen, K. E., Fellous, M., Pellegrini, S. (1996). Interferon-alpha -dependent Activation of Tyk2 Requires Phosphorylation of Positive Regulatory Tyrosines by Another Kinase. J. Biol. Chem. 271: 20494-20500 [Abstract] [Full Text]  
• Li, X., Leung, S., Qureshi, S., Darnell, J. E. Jr., Stark, G. R. (1996). Formation of STAT1-STAT2 Heterodimers and Their Role in the Activation of IRF-1 Gene Transcription by Interferon-alpha. J. Biol. Chem. 271: 5790-5794 [Abstract] [Full Text]  
• Han, Y., Leaman, D. W., Watling, D., Rogers, N. C., Groner, B., Kerr, I. M., Wood, W. I., Stark, G. R. (1996). Participation of JAK and STAT Proteins in Growth Hormone-induced Signaling. J. Biol. Chem. 271: 5947-5952 [Abstract] [Full Text]  
• Horvath, C M, Wen, Z, Darnell, J E (1995). A STAT protein domain that determines DNA sequence recognition suggests a novel DNA-binding domain.. Genes Dev. 9: 984-994 [Abstract]  
• Velazquez, L., Mogensen, K. E., Barbieri, G., Fellous, M., Uzé, G., Pellegrini, S. (1995). Distinct Domains of the Protein Tyrosine Kinase tyk2 Required for Binding of Interferon-alpha/beta and for Signal Transduction. J. Biol. Chem. 270: 3327-3334 [Abstract] [Full Text]  
• Tamai, K., Li, K., Silos, S., Rudnicka, L., Hashimoto, T., Nishikawa, T., Uitto, J. (1995). Interferon-[IMAGE]-mediated Inactivation of Transcription of the 230-kDa Bullous Pemphigoid Antigen Gene (BPAG1) Provides Novel Insight into Keratinocyte Differentiation. J. Biol. Chem. 270: 392-396 [Abstract] [Full Text]  
• Muller, U, Steinhoff, U, Reis, L., Hemmi, S, Pavlovic, J, Zinkernagel, R., Aguet, M (1994). Functional role of type I and type II interferons in antiviral defense. Science 264: 1918-1921 [Abstract]  
• Darnell, J. Jr, Kerr, I., Stark, G. (1994). Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science 264: 1415-1421 [Abstract]  
• Sadowski, H., Shuai, K, Darnell, J. Jr, Gilman, M. (1993). A common nuclear signal transduction pathway activated by growth factor and cytokine receptors. Science 261: 1739-1744 [Abstract]  
• Shuai, K, Stark, G., Kerr, I., Darnell, J. Jr (1993). A single phosphotyrosine residue of Stat91 required for gene activation by interferon-gamma. Science 261: 1744-1746 [Abstract]  
• Shuai, K, Schindler, C, Prezioso, V., Darnell, J. Jr (1992). Activation of transcription by IFN-gamma: tyrosine phosphorylation of a 91-kD DNA binding protein. Science 258: 1808-1812 [Abstract]  
• Russell-Harde, D., Wagner, T. C., Rani, M. R. S., Vogel, D., Colamonici, O., Ransohoff, R. M., Majchrzak, B., Fish, E., Perez, H. D., Croze, E. (2000). Role of the Intracellular Domain of the Human Type I Interferon Receptor 2 Chain (IFNAR2c) in Interferon Signaling. EXPRESSION OF IFNAR2c TRUNCATION MUTANTS IN U5A CELLS. J. Biol. Chem. 275: 23981-23985 [Abstract] [Full Text]  
• Wagner, T. C., Velichko, S., Vogel, D., Rani, M. R. S., Leung, S., Ransohoff, R. M., Stark, G. R., Perez, H. D., Croze, E. (2002). Interferon Signaling Is Dependent on Specific Tyrosines Located within the Intracellular Domain of IFNAR2c. EXPRESSION OF IFNAR2c TYROSINE MUTANTS IN U5A CELLS. J. Biol. Chem. 277: 1493-1499 [Abstract] [Full Text]  
• Ouchi, T., Lee, S. W., Ouchi, M., Aaronson, S. A., Horvath, C. M. (2000). Collaboration of signal transducer and activator of transcription 1 (STAT1) and BRCA1 in differential regulation of IFN-gamma target genes. Proc. Natl. Acad. Sci. USA 97: 5208-5213 [Abstract] [Full Text]  
• Peters, K. L., Smith, H. L., Stark, G. R., Sen, G. C. (2002). IRF-3-dependent, NFkappa B- and JNK-independent activation of the 561 and IFN-beta genes in response to double-stranded RNA. Proc. Natl. Acad. Sci. USA 99: 6322-6327 [Abstract] [Full Text]  
• Yeh, T. C., Dondi, E., Uze, G., Pellegrini, S. (2000). A dual role for the kinase-like domain of the tyrosine kinase Tyk2 in interferon-alpha signaling. Proc. Natl. Acad. Sci. USA 97: 8991-8996 [Abstract] [Full Text]